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The earliest trough concentration predicts the dose of tacrolimus required for remission induction therapy in ulcerative colitis patients
https://doi.org/10.1186/s12876-015-0285-3
© Hiraoka et al.; licensee BioMed Central. 2015
Received: 28 December 2014
Accepted: 20 April 2015
Published: 29 April 2015
Abstract
Background
Oral tacrolimus therapy is effective for refractory ulcerative colitis (UC), but dose adjustment according to the trough concentrations which varies largely among individuals, is required. This study aimed to identify factors to predict the tacrolimus dose required for achieving the target trough level for remission induction of UC.
Methods
Forty-seven consecutive UC patients who were treated with tacrolimus were retrospectively analyzed. Tacrolimus doses were adjusted every 2 or 3 days to achieve trough concentrations of 10–15 ng/mL. The dose required for reaching the target trough level was analyzed based on disease characteristics, course of trough concentrations, and gene polymorphism related to tacrolimus metabolism.
Results
Median daily dose of tacrolimus required for achieving the target trough level was 0.19 (0.07-0.42) mg/kg, and patients were divided into high or low dose group (< 0.2 mg/kg or > 0.2 mg/kg). The value of initial trough concentration/starting dose was higher in the low dose group than in the high dose group (1.35 ng/mL/mg vs. 0.78 ng/mL/mg, p < 0.0001). Although presence of CYP3A5 *1 was more frequently observed in the high dose group, initial trough concentration was the only significant factor for determining requirement of high dose of tacrolimus (OR = 28.0, 95% confidence interval 3.20 – 631).
Conclusions
The most practical predictor of the dose required for achieving the target trough concentration was the trough concentration measured 2 or 3 days after starting tacrolimus therapy. Our findings would make tarcolimus administration for UC safer, easier and more effective.
Keywords
Background
Ulcerative colitis (UC) is an idiopathic chronic inflammatory disorder characterized by manifestations such as diarrhea, rectal bleeding, abdominal pain, fever, anemia, and body weight loss [1]. 5-aminosalicylates (5-ASA) are the basic therapeutic drug, and most patients with mild to moderate UC respond to them. Corticosteroid treatment is considered in patients with more severe symptoms when 5-ASA are not effective. However, intravenous steroids are not effective in 20-30% of patients, and these patients ultimately are likely to require colectomy [2].
Calcineurin inhibitors such as cyclosporine A and tacrolimus have been shown to be effective for treatment of steroid-dependent or steroid-refractory UC patients with moderate to severe activity. Both of the medications were effective for approximately 80% of refractory UC patients [3-7]. To maximize the efficacy, however, these drugs require adjustment of the doses for administration by routinely measuring blood concentrations. To avoid substantial fluctuation in the blood concentrations of cyclosporine, it has to be administered by continuous intravenous infusion. On the other hand, tacrolimus is usually taken orally with sufficient efficacy by monitoring trough blood concentrations of the drug. However, a large variation in the dose required for reaching sufficient blood trough levels among individuals has made the administration of tacrolimus difficult.
Tacrolimus is metabolized primarily by cytochrome P450 3A4 (CYP3A4) and CYP3A5, and converted to a substrate of ATP-binding cassette sub-family B member 1 (ABCB1) [8,9]. It has been reported that single-nucleotide polymorphisms (SNPs) of CYP3A4, CYP3A5, and ABCB1 are correlated with altered pharmacokinetics of tacrolimus in patients with organ transplantation [10,11]. Subjects having the CYP3A5 *1 allele express large amounts of CYP3A5 protein which promote metabolism of tacrolimus [11]. In the treatment of UC, Hirai et al. recently reported that a SNP of CYP3A5 was correlated with not only the period and sufficient dose required for reaching the target trough concentration, but also efficacy of tacrolimus therapy [12]. In clinical settings, however, it is not practical to examine SNPs before tacrolimus administration because of the cost and clinical unavailability. Moreover, whether patients should be treated with tacrolimus is determined regardless of presence of the SNPs because the treatment options for refractory UC are limited. In this context, easy and practical methods to predict optimal dose of tacrolimus for achieving sufficient trough levels are eagerly anticipated.
In this study, therefore, we meticulously examined the courses of trough concentrations of tacrolimus in UC patients who were administered tacrolimus as remission induction therapy, and aimed to identify predictive factors for the dose of tacrolimus required to achieve sufficient trough levels which could be easily used in actual clinical settings.
Methods
Patients and procedures
Data on all patients with moderate to severe UC who were treated with tacrolimus at Okayama University Hospital and Wakayama Medical University Hospital from September 2006 to October 2012, were analyzed. A diagnosis of UC was established by the presence of accepted clinical, endoscopic, and histologic criteria. No patients had any renal function disorders, and if patients received tacrolimus therapy twice or more, only the initial administration episode was included in the analysis. This study was approved by the institutional review board of both institutions. Ethics Committee of Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences and Wakayama Medical University approved the study protocol including DNA analysis (IRB No.218), and written informed consent was received from each patient. There were no conflicts of interest or sponsors of this study.
Medical charts provided clinical information including demographic data, such as age, gender, and duration of the disease, as well as disease status, such as severity of disease, extent of disease, and medications. According to Truelove and Witts’ criteria [13], all patients had either moderate or severe disease, and all were steroid-dependent or steroid-refractory. Steroid-dependency was defined as the inability to completely withdraw systemic steroids because of disease relapse or the occurrence of at least two flare-ups requiring systemic steroid therapy in a six-month period. Steroid-refractoriness indicated little or no improvement after two weeks of systemic steroid therapy (at least 30 mg prednisolone daily).
As candidates of predictive parameters, concomitant medications (5-ASA, corticosteroids, immunomodulator (azathioprine or 6-mercaptopurine) use), bowel movement (frequency/day), diet (fasting or not), and laboratory data including leukocyte count, platelet count, hemoglobin, and C-reactive protein (CRP) at the start of tacrolimus therapy were specifically examined. Tacrolimus dose was analyzed with adjustment by body weight.
Administration and monitoring of tacrolimus
For patients who were treated with oral tacrolimus therapy, capsules (Prograf; Astellas Pharma Inc., Tokyo, Japan) containing 0.5 mg or 1 mg of tacrolimus were used. The initial oral dose of tacrolimus was 0.05-0.15 mg/kg/day twice daily. Whole blood trough concentration of tacrolimus was measured at each hospital once every 2–3 days by the affinity column mediated immunoassay. The dose of tacrolimus was adjusted in the evening according to the trough concentration measured in the morning of the same day. The target whole blood trough concentration was 10–15 ng/mL and dose adjustment was done to achieve the target concentration as soon as possible within 2–3 weeks [6,14,15]. For most of cases, adjustment was made to reach the target within one week. The equations for dose adjustment were as follows: previous dose × (10 ng/mL/blood trough concentration) until 3 days after starting tacrolimus, and previous dose × (12.5 ng/mL/blood trough concentration) more than 3 days after starting tacrolimus.
All responding patients were followed by tapered blood trough levels of 5–10 ng/mL for about 3–6 months.
Evaluation of efficacy of tacrolimus
The efficacy of tacrolimus was evaluated by criteria based on changes in the clinical activity index (CAI) scores proposed by Lichtiger et al [3]. Clinical courses were classified as remission, response, and no response. Remission was defined as a CAI score of 3 or lower within 4 weeks of starting tacrolimus therapy. Response was defined as a CAI score of 4 or higher, but equal to or lower than half the score at the start of tacrolimus therapy. ‘Effective’ case was defined as a patient either in remission or with response. ‘No response’ was defined as a clinical course that was not identified as in remission or with response. All of these definitions were determined regardless of steroid administration status, because relatively short-term effect was evaluated in this study. Adverse events of tacrolimus were counted when reduction of the dose or discontinuation of tacrolimus was needed.
Analysis of SNPs of genes associated with tacrolimus pharmacokinetics
Genetic analysis of CYP3A5 and ABCB1 polymorphisms was performed according to previous reports [16]. In brief, genomic DNA was extracted from peripheral blood using the QiaAmp DNA Mini kit (Qiagen, Valencia, CA, USA). We analyzed the polymorphisms of CYP3A5 (rs776746) and ABCB1 exon 26 (rs1045642) genes using TaqMan® SNP Genotyping Assay (Applied Biosystems, Foster City, CA, USA) with a LightCycler 480 system, as recommended by the manufacturer (Roche Diagnostics, Basel, Switzerland).
Statistical analysis
We used the chi-square test or Fisher’s exact test for univariate analysis and the logistic regression model for multivariate analysis in order to identify factors contributing to the dose of tacrolimus required. Results are shown with calculations for odds ratios (ORs) and 95% confidence intervals (CIs). These analyses were performed using the JMP program (version 8, SAS Institute, Cary, NC, USA). To estimate appropriate cutoff values for age, leukocyte count, platelet count, hemoglobin, and CRP, receiver operating characteristic (ROC) curve analysis was performed. All p values were two-sided and considered significant when less than 0.05.
Results
Clinical characteristics of patients
Characteristics of study population
Total | 47 |
|---|---|
Age, years, median (range) | |
at onset | 30 (11–67) |
at initiation of tacrolimus | 40 (12–70) |
Disease duration, months, median (range) | 43.6 (0.74-336) |
Gender | |
Male | 24 (51%) |
Female | 23 (49%) |
Disease severity | |
Moderate | 34 (72%) |
Severe | 13 (28%) |
Extent of disease | |
Pancolitis | 34 (72%) |
Left-side colitis | 13 (28%) |
Concomitant therapy | |
5-ASA | 42 (89%) |
Corticosteroid use | 42 (89%) |
Immunomodulator use | 13 (28%) |
SNPs analysis | |
CYP3A5 (*1*1/*1*3/*3*3) | 1/12/24 |
ABCB1 exon26 (CC/CT/TT) | 15/14/8 |
The dose of tacrolimus required for the target trough level
The distribution of the dose required for reaching the target trough concentration of tacrolimus/body weight. The median dose required for reaching the target trough concentration of each patient was 0.19 mg/kg, and the peak of the distribution was 0.15 mg/kg – 0.20 mg/kg.
The differences in clinical background between patients of the low dose group and the high dose group
Low dosea < 0.2 mg/kg | High dosea ≥ 0.2 mg/kg | Univariate analysis | |
|---|---|---|---|
n = 26 | n = 21 | p | |
Median(range) age at onset, years | 36 (12–67) | 31 (11–64) | NS |
Median(range) age at starting tacrolimus, years | 42.5 (12–70) | 36 (14–67) | NS |
Median(range) duration of disease, months | 28.7 (0.74-336) | 62.2 (0.97-323) | NS |
Gender | |||
Male/Female | 10/16 | 14/7 | 0.08 |
Disease severity | |||
Moderate/Severe | 20/6 | 14/7 | NS |
Extent of disease | |||
Pancolitis/Left-side colitis | 19/7 | 15/ 6 | NS |
Medications | |||
5-ASA | 25 | 17 | 0.16 |
Corticosteroid use | 24 | 28 | NS |
Dose of corticosteroid at initiation of tacrolimus (PSL) | 25 (0–40) | 20 (0–30) | 0.12 |
Immunomodulator use | 8 | 5 | NS |
Bowel movement at initiation of tacrolimus (times/day) | 8 (4–20) | 7.5 (4–15) | NS |
Diet at initiation of tacrolimus | |||
Fasting | 6 | 6 | NS |
Laboratory data at initiation of tacrolimus | |||
White blood cell count (×103/μL) | 7.52 (4.71-15.0) | 7.50 (5.46-15.2) | NS |
Hemoglobin (mg/dL) | 11.2 (7.50-13.6) | 10.4 (7.90-14.4) | NS |
Platelet count (×104/μL) | 39.6 (9.70-65.6) | 38.1 (12.0-75.2) | NS |
CRP (mg/dL) | 0.93 (0.02-7.73) | 2.20 (0.04-14.7) | 0.053 |
SNPs analysisb | |||
CYP3A5 (*1*1/*1*3/*3*3) | 0/2/19 | 1/10/5 | 0.001 |
ABCB1 exon26 (CC/CT/TT) | 9/8/4 | 6/6/4 | NS |
Clinical courses of patients in the low dose group vs. high dose group
The courses of trough concentrations of tacrolimus in patients belonging to the high-dose group (A) and to the low dose group (B). While the trough concentrations of patients in the high dose group increased gradually without outliers, some of the patients in the low dose group showed excessive trough concentrations early after starting tacrolimus.
Differences in the courses of tacrolimus therapy between patients of the high dose group and low dose group
Low dosea | High dosea | p | |
|---|---|---|---|
n = 26 | n = 21 | ||
Period required for reaching the target trough level (>10 ng/mL) of tacrolimus (day) | 5 (2–13) | 7 (3–17) b | 0.011b |
Patients who attained the target trough level (>10 ng/mL) within 7 days | 23 (88%) | 13 (62%) | 0.043 |
The initial trough concentration/starting dose of tacrolimus (ng/mL/mg) | 1.35 (0.50-3.60) | 0.78 (0.30-1.30) | <0.0001 |
Efficacy | |||
Remission | 18 (70%) | 12(57%) | NS |
Response | 4 (15%) | 7 (33%) | |
No response | 4 (15%) | 2 (10%) | |
Period required for reaching remission (day) | 14 (5–26) | 17.5 (13–27) | 0.0055 |
Adverse events | |||
Required for discontinuation and reduce | 6 (23%) | 2 (10%) | 0.27 |
Predictors of the dose required for achieving the target trough level
As shown in Figure 2, the trough levels measured early from the start of tacrolimus therapy may differentiate between the low dose and high dose groups. In addition, Table 3 indicates that the value of initial trough concentration/starting dose was significantly higher in patients of the low dose group than in those of the high dose group (1.35 (0.50-3.60) vs. 0.78 (0.30-1.30), p < 0.0001). Therefore, the early trough concentration, which can be represented as the value of initial trough concentration/starting dose of tacrolimus, is considered to be one of the predictors of the dose required for achieving the target trough level.
The relationship between initial trough concentrations and doses required for sufficient trough levels. The value of initial trough concentration/starting dose of tacrolimus (ng/mL/mg) and the dose of tacrolimus / body weight (mg/kg) required for reaching the target trough level were inversely correlated (R2 = 0.41, p < 0.001).
Meanwhile, as shown in Table 2 and Figure 3, the patients with CYP3A5 *1 allele (red dot) were more likely to belong to the high dose group, while the patients without this allele (blue dot) were more likely to be classified into the low dose group (presence/absence of CYP3A5 *1 allele: low dose group, 11/5 vs. high dose group, 2/19; p = 0.0003). Therefore, the SNP of CYP3A5 is another candidate predictor of the tacrolimus dose required for achieving the target trough concentration.
Predictive values for patients requiring high tacrolimus doses: the initial trough concentration vs. gene polymorphism
Multivariate analysis of factors predictive for requiring high tacrolimus doses (> 0.2 mg/kg)
Multivariate analysis | ||
|---|---|---|
p | ||
CYP3A5 (*1*1 or *1*3) | 6.14 (0.61 - 66.8) | 0.12 |
The initial trough concentration/starting dose > 1 ng/mL/mga | 28.0 (3.20 - 631) | <0.0001 |
Discussion
Both of the calcineurin inhibitors, cyclosporine and tacrolimus, have been shown to be effective for refractory UC [3-7,12,14]. Whereas cyclosporine has to be administered intravenously due to the variable nature of blood concentration, tacrolimus can be used by peroral administration, usually twice a day, with sufficient efficacy. It has been reported that the optimal trough concentration of 10–15 ng/mL should be attained ‘quickly and safely’ for successful treatment. However, the doses required for reaching the target trough level differ largely among patients, and therefore, management of the trough concentrations is often difficult. Delay in reaching the target trough level would also delay treatment response, and may decrease the efficacy of the drug, whereas too rapid increase in blood concentration could cause adverse events. In this study, to ease the administration of tacrolimus, we looked for markers to predict the dose required for achieving the target blood concentration among materials already available in actual clinical practice.
The algorithm based on the earliest trough concentration. If a patient with body weight of 60 kg started tacrolimus therapy with 6 mg/day, and the trough concentration measured 2 or 3 days after initiation was < 6 ng/mL, the dose should be increased to 12 mg/day. On the other hand, if the trough concentration measured 2 or 3 days after initiation was > 6 ng/mL, the increase of the dose should be minimized
Patients with the CYP3A5 *1 allele are rich in the CYP3A5 protein and can metabolize tacrolimus more efficiently. Therefore, the results regarding the requirement of a higher dose to reach the target trough concentration in patients with CYP3A5 *1 allele appeared to be consistent and corroborative with those of the previous report by Hirai et al [12]. In contrast to the previous report, however, the remission rate within 4 weeks did not differ significantly between the two groups (13/24 (54%) vs. 10/13 (77%), p = 0.29). The discrepancy could be attributable to the following reasons.
First, the patients in the report by Hirai et al. had more severe disease than our cohort; all of their patients were hospitalized, and the average CRP appeared to be more than 5 mg/dL. In contrast, 14/47 (30%) of our patients were outpatients, and the median CRP of our patients was 1.4 mg/dL. Perhaps due to the difference in severity, the total remission rate was higher in our study than in the previous study (30/47 (64%) vs.14/45 (31%)). The higher remission rate of our study was probably one of the contributing factors for lack of statistical significance between patients with and without the SNP. Second, the frequency of adjustment of tacrolimus doses may be different. We measured trough concentrations of tacrolimus once every 2–3 days, while Hirai et al. appeared to measure the trough concentration only twice, on days 2–5 and 7–10, during the early period of therapy. The frequent adjustment of the dose of tacrolimus may have increased the remission rate of our patients. Whether or not our easy method for predicting the dose requirement of tacrolimus is valid in more severe UC patients should be verified in future studies.
It is expected that our results would make the handling of tacrolimus easier and would expand the use of the drug in the clinical situation. Because the treatment tools for refractory UC are still limited, tacrolimus could be a user-friendly option that would impact greatly on the clinical practice for refractory UC patients in several manners. First, while another calcineurin inhibitor, cyclosporine, requires continuous intravenous infusion, tacrolimus can be taken orally, indicating that the drug can be prescribed for outpatients. Our results would make the trough management of tacrolimus therapy for outpatients easier and reduce the frequency of clinic visits, possibly resulting in an increase in the success rate of the treatment. Second, in current clinical situations, anti-tumor necrosis factor alpha antibodies such as infliximab and adalimumab are usually used for refractory UC outpatients. Although such agents have also been shown to be effective for refractory UC patients [17,18], those are injected agents, thus, patients have to visit a clinic or hospital periodically for infusions or perform self-injection. In contrast, tacrolimus has an advantage that it is a peroral drug and easily acceptable for every type of patients. Third, appropriate management of the blood concentration of tacrolimus would reduce adverse events due to the drug. The relatively frequent adverse events of tacrolimus are nausea, headache, tremor, and renal dysfunction. Those events usually occur when blood concentrations of the drug are too high. In particular, nausea and headache are likely to occur when the blood concentration abruptly increases. Reduction of adverse events by appropriate monitoring of the blood concentration as per our results would also lead to increased success of the tacrolimus treatment.
The starting dose of tacrolimus for UC used in the previous phase III study was 0.05 mg/kg/day [6,14]. In this study, the average initial dose of tacrolimus was 0.10 mg/kg/day, although there was variation among patients due to the retrospective cohort. As shown in Figure 1, the dose required for the target trough level was more than 0.10 mg/kg/day in 91% of patients, thus, the initial dose of 0.10 mg/kg/day would be reasonable. In this situation, however, patients of the low dose group more frequently developed adverse events (23%) mostly due to excessive trough concentrations, although most of these events were mild. Therefore, our findings regarding the prediction of requirement of low or high dose within 2 or 3 days after starting tacrolimus would be helpful in view of avoidance of adverse events.
There are limitations to the present study. Because this was a retrospective clinical study, the intervals of trough measurement differed among patients. The initial trough concentration was not measured at 2 or 3 days after start of tacrolimus in several patients, and those patients were not included in the corresponding analyses. Trough management based on our results should be validated in prospective studies in the future. The SNP analysis also could not be performed in a few patients who did not give approval for the analysis.
Conclusions
We found that the initial trough concentration could be a useful predictor of the dose of tacrolimus required for remission induction therapy. The drug monitoring based on our findings can be utilized in any situation of clinical practice and does not need specific examinations such as analysis of gene polymorphism. Our findings would make tacrolimus therapy for refractory UC patients more patient- and physician-friendly.
Abbreviations
- UC:
-
Ulcerative colitis
- 5-ASA:
-
5-aminosalicylates
- CYP3A4:
-
Cytochrome P450 3A4
- ABCB1:
-
ATP-binding cassette sub-family B member 1
- SNPs:
-
Single-nucleotide polymorphisms
- CRP:
-
C-reactive protein
- CAI:
-
Clinical activity index
- ORs:
-
Odds ratios
- CIs:
-
Confidence intervals
- ROC:
-
Receiver operating characteristic
- AZA:
-
Azathioprine
- 6MP:
-
6-mercaptopurine
Declarations
Acknowledgements
The authors would like to acknowledge Ms. Mori for conducting the experimental procedures.
Authors’ Affiliations
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