This article has Open Peer Review reports available.
Poly(ADP-ribose) polymerase inhibition suppresses inflammation and promotes recovery from adrenal injury in a rat model of acute necrotizing pancreatitis
- Jia Yu†1, 2,
- Teng Zuo†1, 2,
- Wenhong Deng1, 2,
- Qiao Shi1, 2,
- Peng Ma1,
- Chen Chen1, 2,
- Liang Zhao1,
- Kailiang Zhao1 and
- Weixing Wang1, 2Email author
© The Author(s). 2016
Received: 26 October 2015
Accepted: 14 July 2016
Published: 27 July 2016
Poly(ADP-ribose) polymerase (PARP) participates in multi-organ failure in various inflammatory diseases including acute necrotizing pancreatitis (ANP). Since pancreatitis-associated adrenal insufficiency is partly caused by inflammatory damage to the adrenal cortex, we examined whether PARP antagonism could alleviate adrenal insufficiency in a rat model of ANP.
ANP was induced by retrograde infusion of sodium taurocholate into the bile-pancreatic duct. At 30 min prior to taurocholate infusion, rats were pretreated with the PARP inhibitor 3-Aminobenzamide (3-AB, 20 mg/kg) or vehicle. Pancreatic pathological injury, adrenal histology, neutrophil infiltration, cell apoptosis, and serum corticosterone level were assessed at various times points. Activities of poly(ADP-ribosyl)ated protein (PAR), nuclear factor-kappaB (NF-kB), tumor necrosis factor-α (TNF-α), intercellular adhesion molecule-1 (ICAM-1) and inducible nitric oxide synthase (iNOS) in the adrenal were also examined.
PARP overactivation in ANP rats is associated with reduced serum corticosterone level and marked cellular alterations in adrenocortical tissue. Inflammatory stress caused by ANP reduced adrenal corticosterone release. 3-AB reduced the activation of PARP and inflammatory markers, decreased myeloperoxidase activity, attenuated adrenal morphologic lesions and cells apoptosis, simultaneously improved the impaired adrenal function.
Our data demonstrate the involvement of PARP overactivation in the pathogenesis of adrenal dysfunction after ANP. PARP inhibition may suppress inflammation and promote functional recovery from adrenal injury.
Relative adrenal insufficiency (RAI), defined as inadequate adrenal corticosteroid production, is common in critically ill patients due to a variety of conditions, including severe acute pancreatitis (SAP) [1–4]. In SAP patients, RAI is associated with increased morbidity and mortality . Dysfunction of the hypothalamic-pituitary-adrenal (HPA) axis is a major contributing factor to adrenal insufficiency under such conditions . Another key factor is organic damage to the adrenal gland. In animal models of acute necrotizing pancreatitis (ANP), adrenal insufficiency could be largely attributed to pathologically verifiable damage to the adrenal gland [6, 7], caused by a variety of pathological processes, including ischemia, hemorrhage, inflammation, and apoptosis [1, 8, 9].
Poly (ADP-ribose) polymerase (PARP) is a key enzyme in DNA repair machinery, and is present in almost all cells [10, 11]. Excessive activation of PARP, however, produces extended chains of ADP-ribose on a variety of nuclear proteins including PARP itself and results in a substantial depletion of intracellular nicotinamide adenine dinucleotide (NAD+) and adenosine triphosphate (ATP), leading to cellular dysfunction and death . PARP-1 activity accounts for approximately 90 % of the total poly(ADP-ribose) (PAR) formation . Poly(ADP-ribosyl)ation performs a multitude of biological functions, including regulation of gene expression and gene amplification, cellular differentiation, cellular division and DNA replication, as well as modulating cell apoptosis .
PARP participates in inflammatory responses by regulating NF-kB-dependent gene expression, such as TNF-α, Interleukin (IL)-1β, IL-6, ICAM-1 and iNOS [14, 15]. These signaling could affect HPA axis function at the levels of corticotrophin-releasing hormone and adrenocorticotropic hormone activity [16, 17], which in turn affects the immune reciprocally, and eventually the survival of SAP patients. Despite of the pathophysiologic links between stress signals delivered to HPA axis and hormonal regulation in the adrenal gland, the underlying intracellular signaling events causing inadequate adrenal function in ANP are poorly understood.
In the current study, we conducted a comprehensive analysis of adrenal function in a rat model of ANP and examined the potential effects of PARP inhibition in adrenal gland.
Male Wistar rats (200–250 g) were purchased from the Experimental Animal Center of Hubei Academy of Medical Sciences (Wuhan, China). 3-Aminobenzamid (3-AB), a PARP inhibitor, was obtained from Sigma (#A0788, St. Louis, MO, USA). Other materials, including sodium taurocholate, were obtained from Sigma-Aldrich, except as otherwise noted.
Adult male rats received a standard diet and water ad libitum, and were cared for in adherence with the National Institutes of Health guidelines on the use of laboratory animals. The study was approved by the Ethics Committee of Wuhan University. Rats received retrograde infusion of 5 % TCA (1 mL/kg) into the biliary-pancreatic duct under chloral hydrate (3 ml/kg) anesthesia . 30 min prior to the infusion, rats randomly received either an intravenous bolus of 3-AB (20 mg/kg, dissolved in 5 % DMSO diluted in saline) or vehicle (5 % DMSO). Upon completion of surgical procedures, rats were randomly allocated into the following three groups: (i) TCA + vehicle group (ANP group, n = 48); (ii) TCA + 3-AB group (3-AB group, n = 48). Rats of ANP and 3-AB groups were sacrificed at 3, 6 (n = 8) and 12, 24 h (n = 20) after TCA infusion to examine the responses during the acute phase, and at 12 h to examine adrenal injury. A group of 10 rats receiving sham surgery and vehicle treatment was also included as an additional control (CON group).
Treatment timing and dosing of 3-AB used were based on the previous study by us to provide beneficial actions against hepatic and renal dysfunction in rats suffering ANP and by others to exert protective effects in experimental models of acute inflammatory response [19, 20].
Surviving rats were sacrificed via intracardiac puncture to obtain pancreatic tissues and adrenal glands. Tissue samples were immersed in 4 % phosphate-buffered formaldehyde for histological examination and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay (n = 5). Some adrenal specimen were processed for electron microscopy (n = 3), or snap-frozen in liquid nitrogen and stored at −80 °C for subsequent analysis (6 for Western blot, 5 for myeloperoxidase assay).
The serum was obtained by centrifugation (3000 rpm × 10 mins) of the blood samples. Activities of amylase and lipase were determined by an automatic dry biochemical analyzer (Vitros-350, Johnson & Johnson Co., Calif, USA). Serum corticosterone concentration in rats (cortisol in humans) was measured using an ELISA kit (Cayman Chem. Co., Ann Arbor, MI, USA).
Histopathologic and TUNEL analysis
Adrenal gland was cut into 2 pieces, one for ultrastructural morphologic analysis using electron microscopy and the other for conventional histopathologic analysis with hematoxylin-eosin staining under a light microscope. Pancreatic histological grading of edema, inflammation, vacuolization and necrosis was quantified by morphometry as previously described . Assessment was conducted by two independent observers.
Adrenocortical hemorrhagic necrosis was examined as described earlier . For each experimental condition, ten magnified fields were analyzed per adrenal section from five different rats. A score from 1 to 4 was given for each adrenocortical profile involving an intersection: grade 1, <25 % necrosis; grade 2, 25–50 % necrosis; grade 3, 50–75 % necrosis; and grade 4, >75 % necrosis. A total score was derived by summation of all 50 scores, with a maximum score of 200, according to the modified method proposed by Chatterjee PK .
Cell apoptosis in adrenal cortex was examined using TUNEL staining with an In Situ Cell Death Detection Kit (Roche, Mannheim, Germany). Briefly, 10-μm sections were prepared from paraffin-embedded tissues and deparaffinized. Sections were treated with 20 μg/ml proteinase K for 15 min, blocked in 3 % H2O2 in methanol for 10 min, permeabilized for 2 min in 0.1 % Triton X-100/sodium citrate at 4 °C, and treated with TUNEL reaction mixture. Sections were exposed to diaminobenzidine for 1 min and dehydrated. Normal nuclei were identified using 1 % methyl green nuclear contrast coloration. Sections were photographed using an Olympus BH-2 microscope. For each section, five fields, chosen at random, were counted.
Electron microscopy analysis of ultrastructure
Adrenal samples were processed for ultrastructural morphologic study under transmission electron microscope (TEM) as previously described . Specimens were fixed in 2 % glutaraldehyde, post-fixed in 1 % osmium tetroxide, dehydrated in a graded acetone series, and embedded with Epon-812. Ultra-thin sections were stained with uranyl acetate and lead citrate using a standard protocol, and then examined under EM.
Western blot analysis
PARP activation was examined by analyzing PAR with Western blot as described previously . Nuclear extract of adrenal tissue was prepared by using a Nuclear-Cytosol Extraction Kit (Applygen, Beijing, China) to analyze nuclear NF-kB proteins. Samples were separated on 8 % or 10 % SDS-PAGE, transferred to a nitrocellulose membrane, and probed overnight at 4 °C with one of the following primary antibodies: rabbit monoclonal anti-poly(ADP-ribose) (1:5000, Alexis Biochemicals, Nottingham, UK), NF-kB p65 (1:2000, Cell Signaling, Beverly, USA), goat polyclonal anti-TNF-α, and goat polyclonal anti-ICAM-1 (Santa Cruz, California, USA). Membranes were washed extensively prior to incubation with a HRP-conjugated secondary goat anti-rabbit antibody (1:5000, Pierce, Rockford, IL, USA). Protein bands were visualized using enhanced chemiluminescence, and analyzed with densitometry (Quantity One 4.5.0 software, Bio-Rad Laboratories, Richmond, CA). Sample protein concentration was determined using a BCA method.
Adrenal MPO activity was examined as described previously . Samples were weighed and homogenized in 1:19 (w/v) in ice-cold homogenization buffer. MPO activity was analyzed with a commercial assay kit (Jiancheng Co., Nanjing, China), and presented as units/g of wet tissue.
All values are expressed as mean ± SD for n observations. In the in vivo studies, n represents the number of animal subjects. In the experiments involving histology or immunohistochemistry, the figures shown are representative of at least 3 experiments performed on different days. Statistical analysis was performed using one-way ANOVA, followed by Bonferroni post-hoc test for multiple comparisons. The Kaplan and Meier method was used for analyzing survival rates. Statistically significance was defined as P < 0.05.
PARP was activated in the adrenal glands after ANP
PARP inhibition reduced pancreatic enzymes and histology
PARP inhibition alleviated adrenal insufficiency, histology and neutrophil infiltration
TAC retrograde infusion caused severe destruction of sinusoids, hemorrhagic necrosis and various types of degeneration (vacuolar and granular degenerations, steatosis) in adrenal glands (Fig. 4e). 3-AB pretreatment apparently reduced histological damages (Fig. 4f and c).
PARP inhibition reduced adrenocortical cell apoptosis and ultrastructure changes
PARP inhibition reduced adrenal PARP, NF-kB and cytokines activation
PARP inhibition improved the survival rate of ANP rats
The present study demonstrated that PARP inhibition with 3-AB could suppress inflammation and promotes recovery from adrenal injury in a rat model of ANP. The TCA retrograde infusion elevated serum corticosterone at the early stage. However, serum corticosterone then rapidly declined to a level below the baseline. Such a profile parallels adrenal insufficiency in many human patients with ANP. These findings suggest a critical role of PARP activation in the pathogenesis of acute adrenal injury.
Interestingly, PARP activation was time-dependent, with a prolonged time-course. Pathophysiological processes such as acute pancreatitis, generate free radicals and oxidant species lead to DNA injury and the activation of PARP [26, 27]. This delayed pattern of PARP activation may be related to the continuing presence of free radical and oxidant production in the injured adrenal (which would, therefore, maintain a continuing trigger for DNA single-strand breakage). Once an extensive amount of PAR polymer is formed, it remains significantly elevated for a prolonged period after a variety of toxic insults . This would also explain the observation that the degree of pancreatitis-associated adrenal injury gradually increased with time.
Our study also demonstrated that the impairment of adrenal function and structure (indication of adrenocortical cell death) in ANP rats were significantly improved after PARP inhibition. Cell death appears to play a role in adrenocortical remodeling during embryonic and postnatal development of primates . Recent publications suggest a role of PARP in necrosis. For example, necrosis caused by H2O2 in intestinal epithelial cells or fibroblasts is inhibited by PARP antagonists or by PARP-1 deficiency . PARP-1 knockout mice are protected from endotoxin-induce lethality and hemorrhagic shock . These data showed that protection from cell death was achieved by prevention of ATP depletion, which was caused by PARP activation. Apoptosis might represent a regulatory mechanism that allows adrenal gland to cope with stresses as well as functional demands . In our study, apoptotic nuclei were observed in adrenal cortex of pancreatitis rats by TUNEL. In addition, our ultrastructural analysis of adrenocortical cells indicates that PARP inhibition attenuated adrenocortical cell apoptosis and improved ultrastructral changes. Steroidogenesis takes place in mitochondria and the smooth endoplasmic reticulum in the steroid-producing cells, depression of serum corticosterone was related to the direct adrenal injury which was characterized by a sequence of degeneration, hemorrhage, necrosis, and apoptosis in the adrenal cortex [1, 7, 33]. Neutrophils infiltration into inflamed tissue play a crucial role in the breakdown and remodeling of injured tissue . We have demonstrated that rats respond to ANP with a significantly higher degree of tissue neutrophils infiltration in the adrenal gland. In our experiments, 3-AB significantly reduced adrenal MPO response to ANP protocol. It is important to note that in experimental AP, the glucocorticoid agonists attenuated the MPO activity. A recent publication underlines the importance of glucocorticoid action in the control of inflammatory mediator-induced remote organ damage during AP . These results underlines that an appropriate adrenal glucocorticoid response to inflammatory stimuli is critical for the organism to cope with disease.
PARP activation contributes importantly to the pathophysiology of the inflammatory response. Soriano et al. reported significantly lower levels of TNF-α and IL-6 together with a reduced degree of cellular necrosis and organ inflammation in PARP-1 deficient mice with peritonitis . A previous study in a secretagogue-induced murine model of acute pancreatitis  demonstrated that PARP-1 antagonism (or deficiency) could reduce damage to the pancreas and the lungs [26, 37]. Conceivably, the protective effects of PARP-1 antagonism in organs other than the pancreas could be secondary to alleviation of pancreatitis. However, we do believe the contribution of direct action of PARP-1. For example, suppression of PARP-1 expression by pharmacological inhibitor or the siRNA protects against oxidative stress-related injuries in cultured human hepatocyte WRL-68 cells .
Our findings indicated that PARP inhibition reduced the activation and the subsequent expression of pro-inflammatory genes (e.g., TNF-α and ICAM-1). PARP influences inflammation by interacting with the transcription factor NF-kB, in the absence of PAR formation. PARP inhibition protected animals from inflammatory injury, which is associated with the impaired transcription of many NF-kB-regulated pro-inflammatory factors . Evidence suggests that these mediators can influence the synthesis of glucocorticoid by interfering the corticotropin-releasing hormone and adrenocorticotropic hormone activity . Our study also demonstrates a significant impairment in adrenal glucocorticoid release in 12 h after pancreatitis. A prospective trial using low-dose glucocorticoid replacement for the treatment of sepsis showed a reduction in mortality with no increase in adverse events . Increasing use of steroid replacement for treatment of sepsis in the intensive care unit has led critical care physicians to use steroids in other disease processes, such as trauma and hemorrhagic shock . In severe pancreatitis, at least in the early phase, several animal experiments have shown improved survival after steroid treatment . Last but not least, the mortality rate of rats in ANP was significantly reduced after PARP inhibition in our experiments, indicating clinical relevance. One limitation with this study is that, according to recent data, 3-AB is not a potent antioxidant . However, we cannot exclude that some additional pharmacological effects of 3-AB, although unlike at used dosage, may have effect on observed results. The various classes of recently emerging potent, specific PARP inhibitors will help to further clarify this question.
In conclusion, PARP overactivation may participate in a cascade of cellular and molecular events associated with of transcription factors activation in the adrenal after injury in ANP. Here, we demonstrate that PARP inhibition could protect against adrenal injury by blocking both adrenocortical cell necrosis/apoptosis and inflammatory factors, thereby collectively improves the impaired adrenal function. These findings encourage future studies of PARP as a site of intervention in the treatment of acute pancreatitis.
3-AB, 3-Aminobenzamide; ANP, acute necrotizing pancreatitis; ATP, adenosine triphosphate; NAD+, nicotinamide adenine dinucleotide; NF-kB, nuclear factor-kappaB; PAR, poly(ADP-ribosyl)ated protein; PARP, poly(ADP-ribose) polymerase; RAI, relative adrenal insufficiency; TCA, sodium taurocholate
We thank Mr. Hong Xia from Key Laboratory of Hubei Province for Digestive System Disease and Central Laboratory for the technical assistance. This work was partially supported by the National Science Foundation of China (NSFC) under Grant Nos. 81070368, 81300356.
Availability of data and materials
The availability of the data and material section concerning the manuscript is related to all the experimental results that have been archived and can be shared if desired.
JY and TZ wrote this paper; JY, TZ and WW designed the research and performed the imaging procedures; QS, CC, LZ, and KZ carried out the animal model and the measurement of studies; QS and PM performed the statistical analysis. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Ethics approval and consent to participate
The ethical approval for the study has been received by the Ethics Committee of Wuhan University concerning the publication of this manuscript.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Yu J, Xu S, Wang WX, Deng WH, Jin H, Chen XY, Chen C, Sun HT. Changes of inflammation and apoptosis in adrenal gland after experimental injury in rats with acute necrotizing pancreatitis. Inflammation. 2012;35:11–22.View ArticlePubMedGoogle Scholar
- De Waele J, Hoste E, Baert D, Hendrickx K, Rijckaert D, Thibo P, Van Biervliet P, Blot S, Colardyn F. Relative adrenal insufficiency in patients with severe acute pancreatitis. Intensive Care Med. 2007;33:1754–60.View ArticlePubMedGoogle Scholar
- Rushing G, Britt R, Britt L. Effects of hemorrhagic shock on adrenal response in a rat model. Ann Surg. 2006;243:652–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Marik PE, Zaloga GP. Adrenal insufficiency during septic shock. Crit Care Med. 2003;31:141–5.View ArticlePubMedGoogle Scholar
- Marik PE. Critical illness-related corticosteroid insufficiency. Chest. 2009;135:181–93.View ArticlePubMedGoogle Scholar
- Muller CA, McArthur N, Belyaev O, Burr W, Werner J, Meiser A, Weyhe D, Buchler MW, Uhl W. The effect of synacthen on acute necrotizing pancreatitis in rats. Pancreas. 2008;37:316–20.View ArticlePubMedGoogle Scholar
- Xu S, Chen C, Wang WX, Huang SR, Yu J, Chen XY. Crucial role of group IIA phospholipase A2 in pancreatitis-associated adrenal injury in acute necrotizing pancreatitis. Pathol Res Pract. 2010;206:73–82.View ArticlePubMedGoogle Scholar
- JÄÄTTELÄ M, ILVESMÄKI V, VOUTILAINEN R, U-H STENMAN, SAKSELA E. Tumor necrosis factor as a potent inhibitor of adrenocorticotropin-induced cortisol production and steroidogenic P450 enzyme gene expression in cultured human fetal adrenal cells. Endocrinology. 1991;128:623–9.View ArticlePubMedGoogle Scholar
- Didenko VV, Wang X, Yang L, Hornsby PJ. Expression of p21(WAF1/CIP1/SDI1) and p53 in apoptotic cells in the adrenal cortex and induction by ischemia/reperfusion injury. J Clin Invest. 1996;97:1723–31.View ArticlePubMedPubMed CentralGoogle Scholar
- Ikai K, Ueda K. Immunohistochemical demonstration of poly (adenosine diphosphate-ribose) synthetase in bovine tissues. J Histochem Cytochem. 1983;31:1261–4.View ArticlePubMedGoogle Scholar
- Cuzzocrea S. Shock, inflammation and PARP. Pharm Res. 2005;52:72–82.View ArticleGoogle Scholar
- Chiarugi A. Poly(ADP-ribose) polymerase: killer or conspirator? The ‘suicide hypothesis’ revisited. Trends Pharmacol Sci. 2002;23:122–9.View ArticlePubMedGoogle Scholar
- Burkle A, Brabeck C, Diefenbach J, Beneke S. The emerging role of poly(ADP-ribose) polymerase-1 in longevity. Int J Biochem Cell Biol. 2005;37:1043–53.View ArticlePubMedGoogle Scholar
- Nakajima H, Nagaso H, Kakui N, Ishikawa M, Hiranuma T, Hoshiko S. Critical role of the automodification of poly(ADP-ribose) polymerase-1 in nuclear factor-kappaB-dependent gene expression in primary cultured mouse glial cells. J Biol Chem. 2004;279:42774–86.View ArticlePubMedGoogle Scholar
- Hassa P, Hottiger M. The functional role of poly (ADP-ribose) polymerase 1 as novel coactivator of NF-kappaB in inflammatory disorders. Cell Mol Life Sci. 2002;59:1534–53.View ArticlePubMedGoogle Scholar
- Burchard K. A review of the adrenal cortex and severe inflammation: quest of the “eucorticoid” state. J Trauma. 2001;51:800–14.View ArticlePubMedGoogle Scholar
- Bornstein SR, Zacharowski P, Schumann RR, Barthel A, Tran N, Papewalis C, Rettori V, McCann SM, Schulze-Osthoff K, Scherbaum WA, et al. Impaired adrenal stress response in Toll-like receptor 2-deficient mice. Proc Natl Acad Sci U S A. 2004;101:16695–700.View ArticlePubMedPubMed CentralGoogle Scholar
- Aho HJ, Koskensalo SM, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholate-induced acute haemorrhagic pancreatitis. Scand J Gastroenterol. 1980;15:411–6.View ArticlePubMedGoogle Scholar
- Watts JA, Grattan RM, Whitlow BS, Kline JA. Activation of poly(ADP-ribose) polymerase in severe hemorrhagic shock and resuscitation. Am J Physiol Gastrointest Liver Physiol. 2001;281:G498–506.PubMedGoogle Scholar
- Mota R, Sánchez-Bueno F, Saenz L, Hernández-Espinosa D, Jimeno J, Tornel P, Martínez-Torrano A, Ramírez P, Parrilla P, Yélamos J. Inhibition of poly (ADP-ribose) polymerase attenuates the severity of acute pancreatitis and associated lung injury. Lab Invest. 2005;85:1250–62.View ArticlePubMedGoogle Scholar
- Schmidt J, Rattner D, Lewandrowski K, Compton C, Mandavilli U, Knoefel W, Warshaw A. A better model of acute pancreatitis for evaluating therapy. Ann Surg. 1992;215:44–56.View ArticlePubMedPubMed CentralGoogle Scholar
- Chatterjee PK, Patel NS, Sivarajah A, Kvale EO, Dugo L, Cuzzocrea S, Brown PA, Stewart KN, Mota-Filipe H, Britti D, et al. GW274150, a potent and highly selective inhibitor of iNOS, reduces experimental renal ischemia-reperfusion injury. Kidney Int. 2003;63:853–65.View ArticlePubMedGoogle Scholar
- de Almeida H, Magalhães MC, Magalhães MM. Age-related changes in the inner zone of the adrenal cortex of the rat—a morphologic and biochemical study. Mech Ageing Dev. 1998;105:1–18.View ArticlePubMedGoogle Scholar
- Drel VR, Pacher P, Stevens MJ, Obrosova IG. Aldose reductase inhibition counteracts nitrosative stress and poly (ADP-ribose) polymerase activation in diabetic rat kidney and high-glucose-exposed human mesangial cells. Free Radic Biol Med. 2006;40:1454–65.View ArticlePubMedPubMed CentralGoogle Scholar
- Chen C, Xu S, Wang W, Ding Y, Yu K, Wang B, Chen X. Rosiglitazone attenuates the severity of sodium taurocholate-induced acute pancreatitis and pancreatitis-associated lung injury. Arch Med Res. 2009;40:79–88.View ArticlePubMedGoogle Scholar
- Mota R, Sanchez-Bueno F, Berenguer-Pina JJ, Hernandez-Espinosa D, Parrilla P, Yelamos J. Therapeutic treatment with poly(ADP-ribose) polymerase inhibitors attenuates the severity of acute pancreatitis and associated liver and lung injury. Br J Pharmacol. 2007;151:998–1005.View ArticlePubMedPubMed CentralGoogle Scholar
- Yu J, Deng W, Wang W, Ding Y, Jin H, Chen C, Chen X, Xiong X, Xu S. Inhibition of poly(ADP-ribose) polymerase attenuates acute kidney injury in sodium taurocholate-induced acute pancreatitis in rats. Pancreas. 2012;41:1299–305.View ArticlePubMedGoogle Scholar
- Andrabi S, Kim N, Yu S, Wang H, Koh D, Sasaki M, Klaus J, Otsuka T, Zhang Z, Koehler R. Poly (ADP-ribose)(PAR) polymer is a death signal. Proc Natl Acad Sci. 2006;103:18308–13.View ArticlePubMedPubMed CentralGoogle Scholar
- Carsia R, Macdonald G, Gibney J, Tilly K, Tilly J. Apoptotic cell death in the rat adrenal gland: an in vivo and in vitro investigation. Cell Tissue Res. 1996;283:247–54.View ArticlePubMedGoogle Scholar
- Gukovskaya A, Pandol S. Cell death pathways in pancreatitis and pancreatic cancer. Pancreatology. 2004;4:567–86.View ArticlePubMedGoogle Scholar
- Liaudet L, Soriano FG, Szabo E, Virag L, Mabley JG, Salzman AL, Szabo C. Protection against hemorrhagic shock in mice genetically deficient in poly(ADP-ribose)polymerase. Proc Natl Acad Sci U S A. 2000;97:10203–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Bozzo A, Soñez C, Mugnaini M, Pastorino I, Rolando A, Romanini M, Gauna H. Chronic stress effects on the apoptotic index of the adrenal cortex of pregnant rats. Biocell (Mendoza). 2006;30:439–45.Google Scholar
- Porter J, Muscato K, Patrick J. Adrenal hemorrhage: a comparison of traumatic and nontraumatic deaths. J Natl Med Assoc. 1995;87:569–71.PubMedPubMed CentralGoogle Scholar
- Cuzzocrea S, Di Paola R, Mazzon E, Genovese T, Muia C, Centorrino T, Caputi AP. Role of endogenous and exogenous ligands for the peroxisome proliferators activated receptors alpha (PPAR-alpha) in the development of inflammatory bowel disease in mice. Lab Invest. 2004;84:1643–54.View ArticlePubMedGoogle Scholar
- Paszt A, Takacs T, Rakonczay Z, Kaszaki J, Wolfard A, Tiszlavicz L, Lazar G, Duda E, Szentpali K, Czako L, et al. The role of the glucocorticoid-dependent mechanism in the progression of sodium taurocholate-induced acute pancreatitis in the rat. Pancreas. 2004;29:75–82.View ArticlePubMedGoogle Scholar
- Soriano FG, Liaudet L, Szabo E, Virág L, Mabley JG, Pacher P, Szabo C. Resistance to acute septic peritonitis in poly (ADP-ribose) polymerase-1-deficient mice. Shock. 2002;17:286–92.View ArticlePubMedGoogle Scholar
- Bowes J, Thiemermann C. Effects of inhibitors of the activity of poly (ADP-ribose) synthetase on the liver injury caused by ischaemia-reperfusion: a comparison with radical scavengers. Br J Pharmacol. 1998;124:1254–60.View ArticlePubMedPubMed CentralGoogle Scholar
- Tapodi A, Debreceni B, Hanto K, Bognar Z, Wittmann I, Gallyas Jr F, Varbiro G, Sumegi B. Pivotal role of Akt activation in mitochondrial protection and cell survival by poly (ADP-ribose) polymerase-1 inhibition in oxidative stress. J Biol Chem. 2005;280:35767–75.View ArticlePubMedGoogle Scholar
- Annane D, Sebille V, Charpentier C, Bollaert P, Francois B, Korach J, Capellier G, Cohen Y, Azoulay E, Troche G. Effect of treatment with low doses of hydrocortisone and fludrocortisone on mortality in patients with septic shock. JAMA. 2002;288:862.View ArticlePubMedGoogle Scholar
- Czapski GA, Cakala M, Kopczuk D, Strosznajder JB. Effect of poly(ADP-ribose) polymerase inhibitors on oxidative stress evoked hydroxyl radical level and macromolecules oxidation in cell free system of rat brain cortex. Neurosci Lett. 2004;356:45–8.View ArticlePubMedGoogle Scholar