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Reproductive factors and oesophageal cancer in Chinese women: a case-control study
© Chen et al; licensee BioMed Central Ltd. 2011
Received: 30 November 2010
Accepted: 9 May 2011
Published: 9 May 2011
Previous studies showed that sex hormone might play a role in the development of oesophageal cancer in Western countries. However, evidence from Chinese populations is still lacking.
We performed a hospital-based case-control study in Guangzhou, China. From June 2006 to May 2009, face-to-face interviews were conducted on 73 cases and 157 controls. Cases were Chinese females with newly diagnosed primary oesophageal cancer. Controls were hospitalized individuals without cancer and frequency matched by age groups. The interviews included questions about childbearing and menarche history, together with potential confounders. Unconditional logistic regression was used to estimate the risk of factors.
Women who had given birth before were not at increased risk compared to childless women (adjusted OR = 1.17, 95% CI: 0.48 ~ 2.85). The risk of oesophageal cancer increased with age at first birth: the adjusted OR for women first giving birth at age 25 or later was 2.02 (95% CI: 1.01 ~ 4.04) compared with those reporting their first birth before age 22. History of spontaneous abortion was not significantly associated with increased risk (adjusted OR = 1.37, 95% CI: 0.49 ~ 3.83). No significant association was observed between menstrual variables (age at menarche, age at menopause, and years of menstruation) and risk of oesophageal cancer.
Giving birth at later age may increase the risk of oesophageal cancer in women. Further studies in Chinese populations with larger sample sizes are still needed.
Worldwide, oesophageal cancer is the eighth most common type of cancer and the sixth most common cause of death from cancer . In China overall, oesophageal cancer ranks second in incidence and it is estimated that almost half of all oesophageal cancer cases in the world are contributed by China . Oesophageal cancer remains an important public health problem in China.
Oesophageal cancer occurs mainly in two histological types, squamous cell carcinoma and adenocarcinoma. Squamous cell carcinoma has always been the dominant histological type of oesophageal cancer in China. However, the incidence of adenocarcinoma has increased rapidly in some developed countries during the past decades. Meanwhile, the incidence of squamous cell carcinoma has been more stable over time in these countries . Such inconsistent secular trends of different histological types of oesophageal carcer in these countries suggested the differences in etiology by histological type, which were also supported by many analytical epidemiological studies [4–6].
One of the most intriguing observations in the incidence of oesophageal cancer is the male predominance, which has not been well explained. In Western countries, the incidence of oesophageal cancer has a male to female ratio of up to 8:1 [7, 8]. This strong male predominance in Western countries is also age-related [9, 10], differs by histological type , but seems unexplained by the differences in the prevalence of some known risk factors between males and females . The male to female ratio of oesophageal cancer incidence rates is much lower in Asian countries. In 2008, the male to female ratio of oesophageal cancer incidence rates was about 2 in China . It is possible that differences in extrinsic exposures, such as cigarette smoking and alcohol drinking, may explain part of the difference in esophageal cancer incidence rates in men and women in China. It was also hypothesized that some intrinsic exposures, such as sex hormone may play a role in the development of oesophageal cancer. This hypothesis has been tested further in Western countries. In a case-control study in England and Scotland , breastfeeding was associated with a 60% reduction in oesophageal adenocarcinoma risk among women. A pooled analysis based on various case-control studies conducted in Italy found a positive trend toward an increase risk of oesophageal cancer with increasing parity . However, in a Swedish case-control study, no association between childbearing and oesophageal adenocarcinoma was found . A recent pooled analysis also suggested that the endogenous reproductive factors such as parity, menstruation history of pregnancy were not statistically significantly associated with oesophageal adenocarcinoma . Findings of previous studies remain inconsistent.
Although incidence of oesophageal cancer in China is among the highest in the world, no evidence is available on the effect of sex hormone on oesophageal cancer risk among Chinese populations. In addition, squamous cell oesophageal cancer represents the majority of oesophageal cancer cases in China. Thus, previous evidences from Western countries mostly concerning the effect of reproductive factors on the risk of oesophageal adenocarcinoma may not apply to Chinese populations. Therefore, we conducted a case-control study in Guangzhou, China, to investigate the long-term effects of reproductive factors on oesophageal cancer risk in women in a Chinese population.
This study was conducted in one large teaching hospital in Guangzhou, China. Guangzhou is a city with moderate incidence rates for oesophageal cancer in China. In 2006, there were totally 257 male and 56 female cases diagnosed with oesophageal cancer in Guangzhou with crude incidence rates of 13.04/100.000 and 2.97/100,000, respectively, showing a male/female incidence ratio of 4.4 . All Chinese female cases with newly diagnosed primary oesophageal cancer between June 2004 and May 2010 in the hospital were invited for face-to-face interviews within two months after diagnosis. All cases recruited in this study were examined endoscopically and histologically confirmed. Among a total of 81 eligible cases, 73 were interviewed with a participation rate of 90%. Controls were randomly selected from people who requested general health examinations in the same hospital during the same period. Controls were required to be without any history of any type of cancer and frequency matched by five-year age groups, with a control-to-case ratio of two, whenever possible. Among a total of 178 eligible controls, 157 were successfully interviewed with a participation rate of 88%.
Trained interviewers conducted face-to-face interviews using a structured questionnaire to collect information on childbearing and menarche history, together with sociodemographic characteristics and potential confounding factors. Collected potential confounders mainly included tobacco smoking, alcohol use, gastroesophageal reflux, anthropometric measures (height and weight), dietary habits, and regular medication. Similar to previous studies [4, 13], history of gastroesophageal reflux was estimated by asking subjects about recurrent heartburn and regurgitation, which are the cardinal symptoms of gastroesophageal reflux, occurring at least once weekly at least 5 years before the diagnosis (for cases) or the interview (for controls). To avoid reverse causality, subjects were asked to report their anthropometric measures five years before the diagnosis (for cases) or the interview (for controls). Approval to conduct this study was granted by the Ethics Committee of Southern Medical University (Code No. EC2005.097) and the Ethics Committee for Clinical Research of the First Affiliated Hospital of Jinan University (Code No. CRE 2006.019). I Informed consent was obtained before each interview.
All statistical analyses were performed using the Statistical Package for the Social Sciences (SPSS) software 13.0 for windows. Chi square or t tests were used to test differences of sociodemographic factors and potential confounders between the cases and controls. Unconditional logistic regression was used to estimate the odds ratios (ORs) and corresponding 95% confidence intervals (CIs) for each exposure variable. The associations between exposure variables and risk of oesophageal cancer were further examined after adjusting for potential confounders using multivariate logistic regression models. Potential confounders entered into the final model included: age, reflux, smoking, alcohol use, education, employment, body mass index, and intake of fresh vegetables and fruits. We also considered further adjustment for aspirin or nonsteroidal anti-inflammatory drugs (NSAID) use but the estimated ORs did not substantially changed. Also because only a very small proportion of the subjects (lower than 10% for both cases and controls) had regular use of such medicines, aspirin or NSAID use was not included in the final models. The cut-off points for number of children born and age at menarche were predefined based on biological considerations. Age at first birth, age at menopause, and years of menstruation were grouped into quartiles using cut-off points among the controls. To see whether the risk estimates differ by histological type, we also re-analyzed the data after restricting cases to those with diagnosis of squamous cell carcinoma.
Selected characteristics of cases and controls
Cases (N = 73)
Controls (N = 157)
Age, years, mean ± SD
63.4 ± 10.3
62.7 ± 11.4
Education less than middle school, n (%) *
Never employed, n (%) *
Smoking, n (%)
High alcohol intake, n (%)
Reflux, n (%) *
Overweight or obesity, n (%)
Low consumption of fresh vegetables, n (%)
Low consumption of fresh fruits, n (%)
Reproductive factors and risk of oesophageal cancer
Reproductive factors and risk of oesophageal cancer among female participants
Controls n (%)
All types of oesophageal cancer
Squamous cell oesophageal cancer
Cases n (%)
Crude OR (95% CI)
Adjusted OR* (95% CI)
Cases n (%)
Crude OR (95% CI)
Adjusted OR* (95% CI)
1.22 (0.53, 2.78)
1.17 (0.48, 2.85)
1.29 (0.54, 3.04)
1.20 (0.47, 3.36)
Number of children born
1 or 2
1.26 (0.71, 2.22)
1.31 (0.72, 2.35)
1.40 (0.58, 3.39)
1.34 (0.67, 2.68)
3 or more
1.02 (0.51, 2.03)
1.03 (0.49, 2.14)
1.06 (0.40, 2.82)
1.02 (0.38, 2.74)
Age at first birth
1.30 (0.61, 2.78)
1.27 (0.54, 2.98)
1.40 (0.64, 3.08)
1.31 (0.50, 3.43)
2.19 (1.05, 4.58)
2.02 (1.01, 4.04)
2.39 (1.11, 5.12)
2.12 (1.02, 4.41)
1.51 (0.77, 2.94)
1.37 (0.49, 3.83)
1.54 (0.77, 3.04)
1.39 (0.50, 3.86)
Age at menarche
0.63 (0.28, 1.43)
0.81 (0.38, 1.86)
0.65 (0.28, 1.51)
0.80 (0.37, 1.72)
2.59 (0.87, 7.71)
2.15 (0.77, 6.00)
2.66 (0.87, 8.11)
2.19 (0.79, 6.07)
Age at menopause
1.31 (0.65, 2.61)
1.12 (0.40, 3.14)
1.29 (0.63, 2.61)
1.10 (0.35, 2.86)
0.92 (0.45, 1.89)
0.96 (0.33, 2.79)
0.89 (0.42, 1.85)
0.94 (0.31, 2.85)
Years of menstruation
0.96 (0.48, 1.92)
0.94 (0.41, 2.15)
0.90 (0.44, 1.84)
0.91 (0.39, 2.12)
0.92 (0.45, 1.86)
0.93 (0.38, 2.27)
0.92 (0.45, 1.90)
0.92 (0.35, 2.42)
The present study was the first one that has been performed in a Chinese population to investigate the effects of reproductive factors on oesophageal cancer risk. This study suggested that giving birth at later age might increase the risk of oesophageal cancer in women. Such findings were in line with those of a previous study in Italy that the OR was 2.5 for women first giving birth at age 30 or later compared with those whose first birth occurred before age 25 . We did not find significant association of oesophageal cancer risk with parity or menarche history. However, a previous analysis based on three case-control studies in Italy and Switzerland showed that the OR was higher for parous than for nulliparous women and age at menopause was inversely associated with squamous cell oesophageal cancer risk . The inconsistency of these studies may be explained by differences in population background, study design, sample size, and also by chance. Further confirmation of existing findings is still needed in future studies.
This study has several major strengths. First, an extensive effort was made to collect information on major risk factors for oesophageal cancer, which was further considered and adjusted throughout the analysis. Second, all cases in this study were histologically confirmed, which minimized misclassification. In addition, in many hospital-based studies, controls were taken from hospitalized individuals with higher chance than the general population to share a common exposure with cases, which may be a threat to the validity of the results. In this study controls were selected from those who came to hospitals for routine health examination, probably making the controls more representative of the general population. However, since it was not a random sample of the general population, there was still a certain risk of selection bias if people seeking routine health examination had any difference in terms of the studied exposures.
Because of the rarity of oesophageal cancer in women, we only had limited number of cases. Actually, other similar studies also suffered from this problem with low statistical power . Increasing the number of controls to a control-case ratio of 4 can, at least to some extent, increase the study power, which needs consideration in future studies. Furthermore, existing evidences showed that the two histological types of oesophageal cancer, squamous cell carcinoma and adenocarcinoma, differ substantially in etiology [4–6]. However, sensitivity analysis restricted to the dominant histological type, squamous cell carcinoma, revealed no strong difference, but the statistical power was even lower. Obviously, studies with larger sample size are still needed.
As the first study to investigate the effects of reproductive factors on oesophageal cancer risk in a Chinese population, this study found suggestive evidence that giving birth at later age might increase the risk of oesophageal cancer in women though this needed confirmation. Further studies in Chinese populations with larger sample sizes are still warranted.
- International Agency for Research on Cancer: Oesophageal Cancer Incidence and Mortality Worldwide in 2008 Summary, GLOBOCAN. 2008, [http://globocan.iarc.fr/factsheets/cancers/oesophagus.asp]Google Scholar
- Parkin DM, Pisani P, Ferlay J: Estimates of worldwide incidence of 25 major cancers in 1990. Int J Cancer. 1999, 80: 827-841. 10.1002/(SICI)1097-0215(19990315)80:6<827::AID-IJC6>3.0.CO;2-P.View ArticlePubMedGoogle Scholar
- Vizcaino AP, Moreno V, Lambert R, Parkin DM: Time trends incidence of both major histological types of esophageal carcinomas in selected countries. Int J Cancer. 2001, 99: 860-868.View ArticleGoogle Scholar
- Lagergren J, Bergstrom R, Lindgren A, Nyren O: Symptomatic gastroesophageal reflux as a risk factor for esophageal adenocarcinoma. N Engl J Med. 1999, 340: 825-831. 10.1056/NEJM199903183401101.View ArticlePubMedGoogle Scholar
- Lindblad M, Luis A, Rodriguez G, Lagergren J: Body mass, tobacco and alcohol and risk of esophageal, gastric cardia, and gastric non-cardia adenocarcinoma among men and women in a nested case-control study. Cancer Causes Control. 2005, 16: 285-294. 10.1007/s10552-004-3485-7.View ArticlePubMedGoogle Scholar
- Holmes RS, Vaughan TL: Epidemiology and pathogenesis of esophageal cancer. Semin Radiat Oncol. 2007, 17: 2-9. 10.1016/j.semradonc.2006.09.003.View ArticlePubMedGoogle Scholar
- Chandanos E, Lagergren J: The mystery of male dominance in oesophageal cancer and the potential protective role of oestrogen. Eur J Cancer. 2009, 45: 3149-3155. 10.1016/j.ejca.2009.09.001.View ArticlePubMedGoogle Scholar
- Corley DA, Buffler PA: Oesophageal and gastric cardia adenocarcinomas: analysis of regional variation using the Cancer Incidence in Five Continents database. Int J Epidemiol. 2001, 30: 1415-1425. 10.1093/ije/30.6.1415.View ArticlePubMedGoogle Scholar
- Derakhshan MH, Liptrot S, Paul J, Brown IL, Morrison D, McColl KEL: Oesophageal and gastric intestinal-type adenocarcinomas show the same male predominance due to a 17 year delayed development in females. Gut. 2009, 58: 16-23. 10.1136/gut.2008.161331.View ArticlePubMedGoogle Scholar
- Ruegard M, Shore R, Lu YX, Lagergren P, Linblad M: Sex differences in the incidence of gastrointestinal adenocarcinoma in Sweden 1970-2006. Eur J Cancer. 2010, 46: 1093-1100. 10.1016/j.ejca.2010.01.029.View ArticleGoogle Scholar
- Cheng KK, Sharp L, McKinney PA, Logan RF, Chilvers CE, Cook-Mozaffari P, Ahamed A, Day NE: A case-control study of oesophageal adenocarcinoma in women: a preventable disease. Br J Cancer. 2000, 83: 127-132.View ArticlePubMedPubMed CentralGoogle Scholar
- La Vecchia C, Negri E, Franceschi S, Parazzini F: Long-term impact of reproductive factors on cancer risk. Int J Cancer. 1993, 53: 215-219. 10.1002/ijc.2910530207.View ArticlePubMedGoogle Scholar
- Lagergren J, Jansson C: Sex hormones and oesophageal adenocarcinoma: influence of childbearing?. Br J Cancer. 2005, 93: 859-861. 10.1038/sj.bjc.6602810.View ArticlePubMedPubMed CentralGoogle Scholar
- Cronin-Fenton DP, Murray LJ, Whiteman DC, Cardwell C, Webb PM, Jordan SJ, Corley DA, Sharp L, Lagergren J: Reproductive and sex hormonal factors and oesophageal and gastric junction adenocarcinoma: a pooled analysis. Eur J Cancer. 2010, 46: 2067-2076. 10.1016/j.ejca.2010.03.032.View ArticlePubMedGoogle Scholar
- Zhao P, Chen WQ: Chinese cancer registry annual report 2009: cancer incidence and mortality in Chinese cancer registration areas in 2006. 2010, Beijing: Military Medical Sciences Press, 110-313.Google Scholar
- Gallus S, Bosetti C, Franceschi S, Levi F, Simonato L, Negri E, La Vecchia C: Oesophageal cancer in women: tobacco, alcohol, nutritional and hormanal factors. Br J Cancer. 2001, 85: 341-345. 10.1054/bjoc.2001.1898.View ArticlePubMedPubMed CentralGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-230X/11/49/prepub
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