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Fibroblast growth factor 23 contributes to diminished bone mineral density in childhood inflammatory bowel disease

BMC Gastroenterology volume 12, Article number: 44 (2012) Cite this article

Abstract

Background

Diminished bone mineral density (BMD) is of significant concern in pediatric inflammatory bowel disease (IBD). Exact etiology is debatable. The recognition of fibroblast growth factor 23 (FGF23), a phosphaturic hormone related to tumor necrosis factor alpha (TNF-α) makes it plausible to hypothesize its possible relation to this pathology.

Methods

In this follow up case control study, BMD as well as serum levels of FGF23, calcium, phosphorus, alkaline phosphatase, creatinine, parathyroid hormone, 25 hydroxy vitamin D3 and 1, 25 dihydroxy vitamin D3 were measured in 47 children with IBD during flare and reassessed in the next remission.

Results

Low BMD was frequent during IBD flare (87.2%) with significant improvement after remission (44.7%). During disease flare, only 21.3% of patients had vitamin D deficiency, which was severe in 12.8%. During remission, all patients had normal vitamin D except for two patients with Crohn’s disease (CD) who remained vitamin D deficient. Mean value of serum FGF23 was significantly higher among patients with IBD during flare compared to controls. It showed significant improvement during remission but not to the control values. 1, 25 dihydroxy vitamin D3, FGF23, serum calcium and urinary phosphorus were significant determinants of BMD in IBD patients.

Conclusions

We can conclude that diminished BMD in childhood IBD is a common multifactorial problem. Elevated FGF23 would be a novel addition to the list of factors affecting bone mineral density in this context. Further molecular studies are warranted to display the exact interplay of these factors.

Peer Review reports

Background

Children with inflammatory bowel disease demonstrate decreased bone mineral density [1]. Risk factors include the use of steroids [2], defective nutritional status [3] and the inflammatory process itself [4]. While some studies recommend the use of oral vitamin D and calcium for prevention of bone loss in patients with IBD [5], no sufficient evidence exists of vitamin D deficiency in these patients [6–8] or of their benefit from vitamin D supplementation [9].

Fibroblast growth factor 23 (FGF23) belongs to the group of phosphatonins, which enhance renal phosphate excretion and inhibit renal 25-hydroxy-vitamin D3 1α hydroxylase [10]. Increased FGF 23 secretion was proved to be the mechanism of bone disease in many diseases including tumor induced osteomalacia [11] and X-linked hypophopshatemic rickets [12]. Decreased degradation of FGF23 was found to be the mechanism of bone disease in autosomal dominant hypophosphatemic rickets [13]. Impaired renal excretion of FGF23 was proved to be the mechanism of renal osteodystrophy and post-transplant hypophosphatemia [14].

The PHEX (phosphate-regulating with homologies to endopeptidases on the X chromosome) gene encodes a Zinc-metalloendopeptidase expressed primarily in osteoblasts and odontoblasts. Studies seemed to confirm the hypothesis of proteolytic degradation of FGF-23 by PHEX [15]. The down-regulation of the PHEX gene results in decreased degradation of FGF-23 and a subsequent increase in its circulating levels [16].

TNF-α is the most recognized cytokine in the pathogenesis of IBD [17]. Uno et al. demonstrated that in experimental animals, TNF-alpha decreases Phex mRNA and protein expression via a transcriptional mechanism and this is at least in part responsible for inhibition of osteoblast mineralization [18].

Hypothesis

We hypothesize that FGF23 is increased in patients with IBD and, being a phosphaturic hormone, may contribute to the diminished BMD noticed in this patient population. So the aim was to assess relation of serum FGF-23 to BMD among children with IBD.

Subjects and methods

This follow up case control clinical study included 47 children with IBD (27 with ulcerative colitis "UC" and 20 with Crohn’s disease "CD") recruited from amongst IBD patients followed up at the Pediatric Gastroenterology Unit, Ain Shams University Faculty of Medicine over the period from December 2008 to December 2010. They were 32 males (14 UC and 18 CD) and 15 females (13 UC and 2 CD), with a mean age of 11.6 ± 3.5 years. Patients were studied during disease activity, either at initial diagnosis (6 UC and 5 CD) or in a disease relapse (21 UC and 15 CD). Patients underwent clinical and laboratory reassessment 3 months after achieving remission depending on feasibility of follow up visits and availability of required tests as DXA scan. The duration between the initial evaluation "flare" and reassessment "remission" ranged between 4 and 9 months (mean of 7.12 +/− 2.8 months). Fifty healthy, age and sex matched children (12 males and 9 females with an age range of 4–16 years and a mean age of 12.8 ± 3.77 years) were included as a control group.

Inclusion criteria

1- Diagnosis of IBD based on the Porto criteria [19].

2- Disease flare that was assessed using Pediatric Ulcerative Colitis Activity Index (PUCAI) for UC [20] and modified Pediatric Crohn’s disease Activity Index (PCDAI) for CD [21].

3- No steroid therapy for at least three months prior to enrollment in this study.

Exclusion criteria

1- Critically ill patients who cannot be transferred for DXA procedure.

2- Concomitant endocrinal, renal or genetic bone diseases.

Plan of work

After approval of the study by the local ethics committee, informed consents were taken from the guardians of all patients, as well as assents from older children.

A full medical history and general examination, including anthropometric assessment of height for age with its Z score as well as body mass index (BMI), were done [22].

Laboratory assessment included complete blood count [Coulter 1660], C-reactive protein [AVITEX- CRP latex test [23]], ESR [Westergren method [24]], serum calcium [colorimetric method [25]], serum alkaline phosphatase [autoanalyzer], serum phosphorus [direct UV method without reduction [26]] and urinary phosphorus [colorimetric methods using an autoanalyzer (Multistat III Plus, Instrumentation Laboratory, Inc, Lexington, MA)]. Colonoscopy and esophago-gastrodudenoscopy [Pentax videosopes, PENYAX GmbH CO. Juulius Vosseler Strasse 104, 22527 Hamburg, Germany] with histopathologic assessment of multiple biopsies were done for all patients. Serum creatinine was measured using Synchron cx7 (Beckman Inc.).

Measurement of 25 (OH) D3 was done using radioiodine (125I)-based RIA kits (DiaSorin, Stillwater, MN) [27] with unit of measurement being ng/ml. Values below 15 ng/ml were considered indicative of vitamin D deficiency [28] and values below 8 ng/ml were considered indicative of severe vitamin D deficiency [29]. Measurement of 1, 25 (OH)2 D3 was done using Human 1, 25-Dihydroxy-Vitamin D RIA Kit (IBL International GmbH) with unit of measurement being pg/ml [30]. PTH level was measured by the Immulite 2000 Intact PTH assay.

Serum FGF23 was measured using a C-terminal human FGF23 ELISA (Immunotopics, San Clemente, CA) [31]. The plasma was stored at –20 C till assessment at end of study. It was expressed as a concentration of pg/ml with a sensitivity of 4 pg/ml.

Bone densitometry

BMD was determined by DXA Lunar scan [DPX-MD 2001, GE medical systems, USA]. Device was calibrated daily, and the technical error calculated to be less than 1%. Z-scores were calculated from BMD values using the reference data for bone density for age and sex [32] and corrected to bone age which was assessed from X Rays of the left hand [33]. The values of total body BMD were used for analysis.Values between −1.0 and −2.5 denoted a mild decrease in BMD and < −2.5 were diagnostic of severe decrease [34].

Treatment

For induction of remission, all patients received oral prednisone (1–2 mg/kg/day) for 3–4 weeks. Parenteral antibiotics and other supportive measures were individually adjusted. After induction of remission, patients were then maintained on 5 amino salicylic acid [35]. Six CD patients subsequently received infliximab as add-on therapy [36]. One patient with UC underwent total proctocolectomy with ileo-anal anastomosis [37]. Remission was defined as PUCAI < 10 points for UC [20] and PCDAI < 15 points for CD [21].

Nutritional support after enrollment in the flare state included calcium (500 – 1000 mg daily) and oral vitamin D3 supplementation as 1000 IU daily for non-deficient and 10000 IU daily for deficient children.

Regarding the physical activity, patients, prior to flare, were not bed ridden but were ambulant and most of them were attending their full-day school activities. The hospital admission ranged between 3–4 weeks. The remaining time till reassessment was spent with their normal range of activity with the exception of strenuous exercises. An exception was one patient with CD who had multiple vertebral fractures with marked decrease of BMD. However we did not quantify physical activities to consider them in analysis.

Statistical methods

The computer program SPSS for Windows, release 10.0 (SPSS, inc, Chicago, IL, USA) was used for data entry and analysis. All quantitative data were analyzed as mean and standard deviation (SD). Student’s t test was used to compare quantitative data between different groups. Chi square test was used to compare qualitative variables between different groups. Multiple regression analysis was performed.

Results

The duration of illness from definitive diagnosis of IBD ranged from 0 to 48 months, while the duration from the first complaint till inclusion in the study ranged from 6 to 60 months.

Patients of the UC group were significantly older than the CD group (12.77 +/−1.71 and 10.49 +/− 3.34 years respectively). Both were comparable to the control group (11.86 +/−3.39 years; p value 0.228 and 0.202 respectively). The male to female ratio was 18:2 in the CD group, and 14:13 in the UC group (p = 0.006).

Bleeding per rectum and joint involvement (arthralgia or arthritis) were more frequently encountered among patients with UC (100%, 48.15% and 48.15% respectively) than CD (35%, 10% and 15% respectively). One of the included patients had pathological fractures of the last 3 lumbar spines.

The mean values of Z score of height-for-age was significantly lower in children with disease activity compared to controls (p < 0.001). Both groups showed a non-significant improvement after remission which remain significantly lower than controls (p < 0.001).

BMI was significantly lower in UC and CD patients during flare (17.26 +/− 2.34 and 17.13 +/− 2.46 respectively) and remission (19.27 +/− 2.07 and 20.27 +/− 3.18 respectively) than in the control group (25.43 +/−2.65) (p < 0.001). The difference between flare and remission was significant for both UC (p = 0.002) and CD (p = 0.001).

Bone mineral density and Z score of corrected BMD to bone age and sex were both significantly lower during disease activity compared to values at remission (Table 1). Severe affection (Z score < −2.5) was present in 88.9% and 75% of patients with active UC and CD respectively. After remission, this decreased to 37% and 10% of patients with UC and CD, respectively (Table 2). The rate of change of Z score of corrected BMD to bone age showed no significant difference between the UC and CD groups (Table 3).

Table 1 Comparisons of biochemical laboratory bone markers among patients and controls
Full size table
Table 2 Frequency of osteopenia and osteoporosis in flare and remission of UC and CD patients
Full size table
Table 3 Comparison of rate of changes of Z score of BMD between remission and flare among patients with UC and CD
Full size table

Serum calcium was significantly lower in UC and CD patients during both disease activity (9.45 +/− 0.45 and 9.26 +/− 0.34 mg/dl respectively) and after disease remission (9.55 +/− 0.43 and 9.500 +/− 0.67 mg/dl respectively) compared to the control group (9.78 +/− 0.26 mg/dl) (p < 0.001). Although mean serum calcium increased after remission, it was not statistically different from that during remission (p > 0.05 in both groups).

Serum phosphorus was significantly lower during disease activity in UC and CD patients (3.40 +/− 1.14 and 3.57 +/− 0.93 mg/dl respectively) compared to remission values (5.16 +/− 0.54 and 5.10 +/− 0.48 mg/dl respectively) and the control group (5.22 +/− 0.48 mg/dl) (p < 0.001). Values during remission were not different from those of controls (p > 0.05).

Serum alkaline phosphatase was significantly higher in UC and CD patients during flare compared to values during remission and controls with p values of <0.0001 for all. However, remission values of both groups were not different from control group.

Similarly, urinary phosphorus was significantly higher during flare in both UC and CD patients (2.01 +/− 0.77 and 2.06+/− 0.69 g/24 hours urinary output respectively) compared to remission (0.66 +/− 0.26 and 0.61+/− 0.18 g/24 hours urinary output respectively) and controls (0.74 +/− 0.19 g/24 hours urinary output) (p < 0.001). Values during disease remission were not different from control ones.

Serum creatinine was not different between cases (flare or remission) and controls.

The 25 hydroxy vitamin D3 values were significantly lower during disease flare compared to control values. Remission values were not significantly different from values during disease activity, or from control values (Table 1). On the other hand, 1, 25 (OH)2 vitamin D3 values were significantly higher during flare of UC and CD compared to controls (Table 1).

FGF23 serum levels were significantly higher during disease flare in UC and CD patients compared to controls. Although values significantly decreased during remission, they remained higher than controls (Table 1).

Serum parathyroid hormone was significantly higher in UC flare and remission as well as CD flare and remission than controls. Flare of UC showed significant higher values than their remission values (p = 0.019). Flare and remission values in CD were not different. Comparison of UC versus CD showed no significant differences.

Regression analysis in the ulcerative colitis group during flare showed the only significant determining factors were FGF23 followed by serum calcium. Regression analysis of BMD in CD group during flare (adjusted R2 = 0.971) showed many significant determining factors affecting BMD. On top comes 1, 21 (OH)2 VD, followed by urinary phosphorus and FGF23 (P <0.0001 for all).

Although the number of CD patients treated with infliximab is very small; the analysis of their BMD and FGF23 showed interesting results. Z score for BMD showed dramatic improvement to normal values in the 6 patients (0.89 +/− 0.67) compared to their flare (−3.32 +/− 2.31) with p value <0.0001. They showed simultaneous decrease of FGF23 to completely normal values in remission (15.38 +/− (3.11)) compared to flare (69.23 +/− (19.34)) with a p value <0.0001.

Discussion

BMD

The diminished BMD during flare with significant improvement in remission agrees with previous similar results in IBD patients using either total body or lumbar spine bone mineral density [38–40]. Herzog et al. [41] and Ahmed et al. [42] reported significant values of 26% and 22% respectively. Schmidt et al. [40] showed no difference between UC and CD regarding the frequency of low BMD.

This degree of improvement was achieved in a period of 7.12 +/− 2.8 months between the flare and remission assessment. Improvement of BMD in such a relatively short period is consistent with Viapiana et al. [43] who found that a 3 months period of intensive nutritional therapy in anorexia nervosa was sufficient to show the significant improvement in BMD. Similarly, Bhambri et al. [44] found that BMD measured by DXA was significantly improved but not to base line after therapy for 2.8+/−1.4 months.

The residual diminished BMD in a significant proportion of IBD children, during remission, suggests that nutritional therapy, IBD specific therapy and/or their duration are not sufficient to restore BMD to complete normality.

Vitamin D

The mean serum level of 25 (OH) vitamin D3, as a measure of vitamin D status [45], showed significantly lower values during flare of UC and CD compared to controls. Deficiency (< 15 ng/ml) or severe deficiency (< 8 ng/ml) were uncommon among patients with IBD during flare and markedly improved after remission. The vitamin D nutritional state returned to normal after achieving disease remission, although the intake of vitamin D and calcium had not changed. This may indicate that the state of hypovitaminosis D is a reflection of disordered bioavailability with disease activity rather than isolated nutritional deficiency. The normalization of vitamin D nutritional status with residual low bone mineral density in IBD patients implies that VD is not the sole player in the development of IBD osteopathy. Sentongo et al. [28] found that there was no association between hypovitaminosis D and bone mineral density (P = 0.10) and Hessov et al. [6] found reduction in trabecular bone mass in the presence of normal mean serum levels of the three vitamin D metabolites (25-hydroxyvitamin D3, 24,25-dihydroxyvitamin D3, and 1,25-dihydroxyvitamin D3) after ileal resection in CD. Higher values of 1,25- dihydroxyvitamin D3 during flare of UC and CD may be due to inflammation-induced intestinal over-expression of activating enzymes. Abreu et al. [8] also demonstrated high levels of 1,25- dihydroxyvitamin D3 in IBD patients, more so in CD patients. They explained this by demonstrating an increased expression of intestinal 1 alpha hydroxylase in CD patients which would contribute to increased activation of vitamin D. This may be reinforced by our finding that levels of active form were dramatically reduced to control values or even less after achieving disease remission.

FGF23

FGF23 was significantly higher during disease flare in both UC and CD groups, and while significantly decreasing during remission, did not return back to normal control values. Although phosphatonins including FGF23 inhibit 1 α hydroxylase enzyme in the kidney [10] yet, the high levels of active vitamin D in this study minimizes this role in our context

This finding was not reported in previous clinical studies on IBD patients. It would be related to the high values of TNF alpha reported in such patients [17]. Uno et al. [18] demonstrated that in experimental animals, TNF-alpha decreases Phex mRNA and protein expression via a transcriptional mechanism and this is at least in part responsible for inhibition of osteoblast mineralization. The Phex mRNA and protein is known to be involved in degradation of FGF23 [46]. So we can propose that higher values of FGF23 with its phosphaturic action [10] and the resultant hypophosphatemia may share, among other factors, in the process of diminished BMD in these particular patients. The high levels of FGF23 cannot be attributed to impaired renal functions as serum creatinine was within normal for all patients. 1,25-dihydroxyvitamin D3 may exert its largest effect on FGF23 expression/production when exposed to high levels of extracellular Pi in osteoblasts/osteocytes [47].

PTH

Parathyroid hormone was found to be significantly higher during disease flare than in remission. Moreover, remission values are still significantly higher than in controls. The relation of PTH to bone metabolism is established. This establishes the role of altered PTH in the cascade of osteopathy seen in these cases. The FGF23 polypeptide also suppresses the expression of vitamin D 1-hydroxylase and PTH, resulting in a reduction in serum 1,25-dihydroxyvitamin D3[48] and PTH [49] levels. So our results, showing high 1, 25(OH)2 VD3 and PTH, suggest that FGF23 elevation is a secondary event rather than a primary incident. The TNF alpha role may augment this elevation through decrease of FGF23 degradation.

The predictors of decreased BMD

The etiology of IBD related bone disease seems to be multi-factorial with inflammation itself being the most important factor [4]. In our present work, bone mineral density was low during disease flare despite absence of steroid therapy for at least 3 months prior to enrolment. It dramatically increased after remission even with the use of steroids in all patients. This may suggest a lesser role of steroids on the bone mineral density in IBD patients. Walther et al. [50] found a similar proportion of osteoporosis in steroid-naive (12%) and steroid-treated (11%) pediatric IBD patients. Paganelli et al. [4] reported that the correlation between the lifetime cumulative dose of steroids or duration of treatment and BMD was not statistically significant.

Regression analysis for different clinical and laboratory variables in the ulcerative colitis showed that the only significant determining factors were FGF23 followed by serum calcium. On the other hand in CD, it showed many significant determining factors affecting BMD. On top comes 1,25-dihydroxyvitamin D3, followed by urinary phosphorus and FGF23. The significant role of vitamin D in CD rather than in UC group may reflect a problem related to inflammation or absorption in the former group.

Previous studies have found that BMI correlated with BMD in IBD both in children [39]. But our analysis did not show an impact of BMI on the BMD in view of other variables.

The marked improvement noted in our CD group might be related to use of infliximab, a TNF inhibitor, in some of them. Franchimont et al. [51] and Ryan et al. [52] found that Infliximab rapidly improves bone formation in adults with IBD.

Controversy exists about the relation between decreased BMD and fracture risk among children with IBD [53]. However, low BMD during childhood is associated with increased fracture risk later in life [54]. Bone metabolism in children is characterized by predominant bone modeling with simultaneous activity of both osteoblasts and osteoclasts on different parts of the bone. Therefore, the principles of bone loss observed in adult patients with chronic inflammation are not directly applicable to children [55]. Understanding the specific underlying mechanisms for decreased BMD among children with IBD may help to implement more effective therapies for this complication.

Conclusion

We can conclude that BMD is significantly low in IBD during disease flare with significant improvement after remission. This osteopathy is multifactorial with a proposed new place for the elevated FGF23 levels in such a complex interplay.

Abbreviations

IBD:

Inflammatory bowel disease

BMD:

Bone mineral density

FGF23:

Fibroblast growth factor 23

CD:

Crohn’s disease

TNF-α:

Tumor necrosis factor alpha

PHEX:

Phosphate-regulating with homologies to endopeptidases on the X chromosome

UC:

Ulcerative colitis

PUCAI:

Pediatric ulcerative colitis activity index

PCDAI:

Pediatric Crohn’s disease activity index

BMI:

Body mass index.

References

  1. Sylvester FA, Wyzga N, Hyams JS, Davis PM, Lerer T, Vance K: Natural history of bone metabolism and bone mineral density in children with inflammatory bowel disease. Inflamm Bowel Dis. 2007, 13 (1): 42-50. 10.1002/ibd.20006.

    Article  PubMed  Google Scholar 

  2. Cowan FJ, Warner JT, Dunstan FD, Evans WD, Gregory JW, Jenkins HR: Inflammatory bowel disease and predisposition to osteopenia. Arch Dis Child. 1997, 76 (4): 325-329. 10.1136/adc.76.4.325.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Ezzat Y, Hamdy K: The frequency of low bone mineral density and its associated risk factors in patients with inflammatory bowel diseases. Int J Rheum Dis. 2010, 13 (3): 259-265. 10.1111/j.1756-185X.2010.01542.x.

    Article  PubMed  Google Scholar 

  4. Paganelli M, Albanese C, Borrelli O, Civitelli F, Canitano N, Viola F, Passariello R: Cucchiara S Inflammation is the main determinant of low bone mineral density in pediatric inflammatory bowel disease. Inflamm Bowel Dis. 2007, 13 (4): 416-423. 10.1002/ibd.20039.

    Article  PubMed  Google Scholar 

  5. Siffledeen JS, Fedorak RN, Siminoski K, Jen H, Vaudan E, Abraham N, Steinhart H, Greenberg G: Randomized trial of etidronate plus calcium and vitamin D for treatment of low bone mineral density in Crohn's disease. Clin Gastroenterol Hepatol. 2005, 3 (2): 122-132. 10.1016/S1542-3565(04)00663-9.

    Article  CAS  PubMed  Google Scholar 

  6. Hessov I, Mosekilde L, Melsen F, Fasth S, Hultén L, Lund B, Lund B, Sørensen OH: Osteopenia with normal vitamin D metabolites after small-bowel resection for Crohn's disease. Scand J Gastroenterol. 1984, 19 (5): 691-696.

    CAS  PubMed  Google Scholar 

  7. Bernstein CN, Bector S, Leslie WD: Lack of relationship of calcium and vitamin D intake to bone mineral density in premenopausal women with inflammatory bowel disease. Am J Gastroenterol. 2003, 98 (11): 2468-2473. 10.1111/j.1572-0241.2003.07676.x.

    Article  CAS  PubMed  Google Scholar 

  8. Abreu MT, Kantorovich V, Vasiliauskas EA, Gruntmanis U, Matuk R, Daigle K: Measurement of vitamin D levels in inflammatory bowel disease patients reveals a subset of Crohn's disease patients with elevated 1,25-dihydroxyvitamin D and low bone mineral density. Gut. 2004, 53 (8): 1129-1136. 10.1136/gut.2003.036657.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Bernstein CN, Seeger LL, Anton PA, Artinian L, Geffrey S, Goodman W, Belin TR, Shanahan F: A randomized, placebo-controlled trial of calcium supplementation for decreased bone density in corticosteroid-using patients with inflammatory bowel disease: a pilot study. Aliment Pharmacol Ther. 1996, 10 (5): 777-786. 10.1046/j.1365-2036.1996.63205000.x.

    Article  CAS  PubMed  Google Scholar 

  10. Shimada T, Mizutani S, Muto T, Yoneya T, Hino R, Takeda S, Takeuchi Y, Fujita T, Fukumoto S, Yamashita T: Cloning and characterization of FGF23 as a causative factor of tumor-induced osteomalacia. Proc Natl Acad Sci U S A. 2001, 98 (11): 6500-6505. 10.1073/pnas.101545198.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Takeuchi Y, Suzuki H, Ogura S, Imai R, Yamazaki Y, Yamashita T, Miyamoto Y, Okazaki H, Nakamura K, Nakahara K, Fukumoto S, Fujita T: Venous sampling for fibroblast growth factor-23 confirms preoperative diagnosis of tumor-induced osteomalacia. J Clin Endocrinol Metab. 2004, 89 (8): 3979-3982. 10.1210/jc.2004-0406.

    Article  CAS  PubMed  Google Scholar 

  12. Jonsson KB, Zahradnik R, Larsson T, White KE, Sugimoto T, Imanishi Y, Yamamoto T, Hampson G, Koshiyama H, Ljunggren O, Oba K, Yang IM, Miyauchi A, Econs MJ, Lavigne J, Juppner H: Fibroblast growth factor 23 in oncogenic osteomalacia and Xlinked hypophosphatemia. N Engl J Med. 2003, 348: 1656-1663. 10.1056/NEJMoa020881.

    Article  CAS  PubMed  Google Scholar 

  13. Shimada T, Muto T, Urakawa I, Yoneya T, Yamazaki Y, Okawa K, Takeuchi Y, Fujita T, Fukumoto S, Yamashita T: Mutant FGF-23 responsible for autosomal dominant hypophosphatemic rickets is resistant to proteolytic cleavage and causes hypophosphatemia in vivo. Endocrinology. 2002, 143 (8): 3179-3182. 10.1210/en.143.8.3179.

    Article  CAS  PubMed  Google Scholar 

  14. Pande S, Ritter CS, Rothstein M, Wiesen K, Vassiliadis J, Kumar R, Schiavi SC, Slatapolsky E, Brown AJ: FGF-23 and sFRP-4 in chronic kidney disease and post-renal transplantation. Nephron Physiol. 2006, 104 (1): p23-p32. 10.1159/000093277.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Bowe AE, Finnegan R, de Beur SM Jan, Cho J, Levine MA, Kumar R, Schiavi SC: FGF-23 inhibits renal tubular phosphate transport and is a PHEX substrate. Biochem Biophys Res Commun. 2001, 284: 977-981. 10.1006/bbrc.2001.5084.

    Article  CAS  PubMed  Google Scholar 

  16. Xia W, Meng X, Jiang Y, Li M, Xing X, Pang L, et al: Three novel mutations of the PHEX gene in three Chinese families with X-linked dominant hypophosphatemic rickets. Calcif Tissue Int. 2007, 81 (6): 415-420. 10.1007/s00223-007-9067-4. Epub 2007 Nov 29

    Article  CAS  PubMed  Google Scholar 

  17. Noguchi M, Hiwatashi N, Liu Z, Toyota T: Secretion imbalance between tumour necrosis factor and its inhibitor in inflammatory bowel disease. Gut. 1998, 43: 203-209. 10.1136/gut.43.2.203.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Uno JK, Kolek OI, Hines ER, Xu H, Timmermann BN, Kiela PR, Ghishan FK: The role of tumor necrosis factor alpha in down-regulation of osteoblast Phex gene expression in experimental murine colitis. Gastroenterology. 2006, 131 (2): 497-509. 10.1053/j.gastro.2006.05.020.

    Article  CAS  PubMed  Google Scholar 

  19. Working IBD: Group of the European Society for Paediatric Gastroenterology, Hepatology and Nutrition.: Inflammatory bowel disease in children and adolescents: recommendations for diagnosis–the Porto criteria. J Pediatr Gastroenterol Nutr. 2005, 41 (1): 1-7. 10.1097/01.MPG.0000163736.30261.82.

    Article  Google Scholar 

  20. Turner D, Otley AR, Mack D, Hyams J, de Bruijne J, Uusoue K, Walters TD, Zachos M, Mamula P, Beaton DE, Steinhart AH, Griffiths AMl: Development, validation, and evaluation of a pediatric ulcerative colitis activity index: a prospective multicenter study. Gastroenterology. 2007, 133 (2): 423-432. 10.1053/j.gastro.2007.05.029. Epub 2007 May 21

    Article  PubMed  Google Scholar 

  21. Shepanski MA, Markowitz JE, Mamula P, Hurd LB, Baldassano RN: Is an abbreviated Pediatric Crohn’s disease activity index better than the original?. J Pediatr Gastroenterol Nutr. 2004, 39: 68-72. 10.1097/00005176-200407000-00014.

    Article  PubMed  Google Scholar 

  22. WHO: a: Child growth standards: length/height-for-age, weight-for-age, weight-for-length, weight-for-height and body mass index-for-age: methods and development. 2006, WHO, Geneva

    Google Scholar 

  23. Wadsworth C, Wadsworth E: Efficacy of latex agglutination and quantification methods for determination of C-reactive protein (CRP) in pediatric sera. Clin Chim Acta. 1984, 138 (3): 309-318. 10.1016/0009-8981(84)90138-4.

    Article  CAS  PubMed  Google Scholar 

  24. Ray AK: A safe and inexpensive method for setting up the Westergren ESR. J Clin Pathol. 1972, 25 (4): 366-10.1136/jcp.25.4.366.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Chapoteau E, Czech BP, Zazulak W, Kumar A: New reagent for colorimetric assay of calcium in serum. Clin Chem. 1993, 39 (9): 1820-1824.

    CAS  PubMed  Google Scholar 

  26. Rubino L, Catapano V, Guerra G: Determination of inorganic phosphorus in serum: Evaluation of three methods applied to the Technicon RA-1000 analyzer. J Automat Chem. 1989, 11 (4): 164-167. 10.1155/S1463924689000350.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Hollis BW, Kamerud JQ, Selvaag SR, Lorenz JD, Napoli JL: Determination of vitamin D status by radioimmunoassay with an125I-labeled tracer. Clin Chem. 1993, 39: 529-533.

    CAS  PubMed  Google Scholar 

  28. Sentongo TA, Semaeo EJ, Stettler N, Piccoli DA, Stallings VA, Zemel BS: Vitamin D status in children, adolescents, and young adults with Crohn’s disease. Am J Clin Nutr. 2002, 76: 1077-1081.

    CAS  PubMed  Google Scholar 

  29. Gordon CM, DePeter KC, Feldman HA, Grace E, Emans SJ: Prevalence of vitamin D deficiency among healthy adolescents. Arch Pediatr Adolesc Med. 2004, 158: 531-537. 10.1001/archpedi.158.6.531.

    Article  PubMed  Google Scholar 

  30. Clive DR, Sudhaker D, Giacherio D, Gupta M, Schreiber MJ, Sackrison JL, MacFarlane GD: Analytical and clinical validation of a radioimmunoassay for the measurement of 1,25 dihydroxy vitamin D. Clin Biochem. 2002, 35 (7): 517-521. 10.1016/S0009-9120(02)00378-8.

    Article  CAS  PubMed  Google Scholar 

  31. Imel EA, Peacock M, Pitukcheewanont P, Heller HJ, Ward LM, Shulman D: Sensitivity of fibroblast growth factor 23 measurements in tumor-induced osteomalacia. J Clin Endocrinol Metab. 2006, 91 (6): 2055-2061. 10.1210/jc.2005-2105. Epub 2006 Mar 21

    Article  CAS  PubMed  Google Scholar 

  32. van der Sluis IM, de Ridder MA, Boot AM, Krenning EP, de Muinck Keizer-Schrama SM: Reference data for bone density and body composition measured with dual energy x ray absorptiometry in white children and young adults. Arch Dis Child. 2002, 87: 341-347. 10.1136/adc.87.4.341.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Greulich WW, Pyle SI: Radiographic Atlas of Skeletal Development of Hand and Wrist. 1959, Stanford University Press, Stanford

    Google Scholar 

  34. Barnes C, Wong P, Egan B, Cameron F, Jones G, Ekert H, Monagle P: Reduced bone density among children with severe hemophilia. Pediatrics. 2004, 114: 177-181. 10.1542/peds.114.1.177.

    Article  Google Scholar 

  35. Buller H, Chin S, Kirschner B, Kohn J, Markowitz J, Moore D, Murch S, Taminiau J: Inflammatory Bowel Disease in Children and Adolescents: Working Group Report of the First World Congress of Pediatric Gastroenterology, Hepatology, and Nutrition. J Pediatr Gastroenterol Nutr. 2002, 35: S151-S158.

    Article  PubMed  Google Scholar 

  36. D'haens G, Van Deventer S, Van Hogezand R, Chalmers D, Kothe C, Baert F: Endoscopic and histological healing with infliximab anti-tumor necrosis factor antibodies in Crohn’s disease: a European multicenter trial. Gastroenterology. 1999, 116: 1029-1034. 10.1016/S0016-5085(99)70005-3.

    Article  PubMed  Google Scholar 

  37. Alexander F, Sarigol S, DiFiore J: Fate of the pouch in 151 pediatric patients after ileal pouch anal anastomosis. J Pediatr Surg. 2003, 38 (1): 78-82. 10.1053/jpsu.2003.50015.

    Article  PubMed  Google Scholar 

  38. Semeao EJ, Jawad AF, Zemel BS, Neiswender KM, Piccoli DA, Stallings VA: Bone mineral density in children and young adults with Crohn’s disease. Inflamm Bowel Dis. 1999, 5: 161-166.

    Article  CAS  PubMed  Google Scholar 

  39. Levine A, Mishna L, Ballin A, Givoni S, Dinari G, Hartman C: Use of Quantitative Ultrasound to Assess Osteopenia in Children With Crohn’s Disease. J Pediatr Gastroenterol Nutr. 2002, 35: 169-172. 10.1097/00005176-200208000-00012.

    Article  PubMed  Google Scholar 

  40. Schmidt S, Mellström D, Norjavaara E, Sundh SV, Saalman R: Low bone mineral density in children and adolescents with inflammatory bowel disease: A population-based study from Western Sweden. Inflamm Bowel Dis. 2009, 15 (12): 1844-1850. 10.1002/ibd.20962.

    Article  PubMed  Google Scholar 

  41. Herzog D, Bishop N, Glorieux F, Seidman EG: Interpretation of bone mineral density values in pediatric Crohn's disease. Inflamm Bowel Dis. 1998, 4: 261-267. 10.1002/ibd.3780040402.

    CAS  PubMed  Google Scholar 

  42. Ahmed SF, Horrocks IA, Patterson T, Zaidi S, Ling SC, McGrogan P: Bone mineral assessment by dual energy X-ray absorptiometry in children with inflammatory bowel disease: evaluation by age or bone area. J Pediatr Gastroenterol Nutr. 2004, 38: 276-281. 10.1097/00005176-200403000-00008.

    Article  CAS  PubMed  Google Scholar 

  43. Viapiana O, Gatti D, Dalle Grave R, Todesco T, Rossini M, Braga V, et al: Marked increases in bone mineral density and biochemical markers of bone turnover in patients with anorexia nervosa gaining weight. Bone. 2007, 40 (4): 1073-1077. 10.1016/j.bone.2006.11.015. Epub 2007 Jan 19

    Article  CAS  PubMed  Google Scholar 

  44. Bhambri R, Naik V, Malhotra N, Taneja S, Rastogi S, Ravishanker U, et al: Changes in bone mineral density following treatment of osteomalacia. J Clin Densitom. 2006, 9 (1): 120-127. 10.1016/j.jocd.2005.11.001. Epub 2006 Mar 27

    Article  PubMed  Google Scholar 

  45. Zerwekh JE: Blood biomarkers of vitamin D status. Am J Clin Nutr. 2008, 87 (4): 1087S-1091S.

    CAS  PubMed  Google Scholar 

  46. Fukumoto S: Post-translational modification of Fibroblast Growth Factor 23. Ther Apher Dial. 2005, 9 (4): 319-322. 10.1111/j.1744-9987.2005.00289.x.

    Article  CAS  PubMed  Google Scholar 

  47. Yamamoto R, Minamizaki T, Yoshiko Y, Yoshioka H, Tanne K, Aubin JE, et al: 1 alpha,25-dihydroxyvitamin D3 acts predominately in mature osteoblasts under conditions of high extracellular phosphate to increase fibroblast growth factor 23 production in vitro. J Endocrinol. 2010, 206 (3): 279-286. 10.1677/JOE-10-0058. Epub 2010 Jun 8

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  48. Shimada T, Hasegawa H, Yamazaki Y, Muto T, Hino R, Takeuchi Y, et al: FGF-23 is a potent regulator of vitamin D metabolism and phosphate homeostasis. J Bone Miner Res. 2004, 19: 429-435.

    Article  CAS  PubMed  Google Scholar 

  49. Ben-Dov IZ, Galitzer H, Lavi-Moshayoff V, Goetz R, Kuro-o M, Mohammadi M, et al: The parathyroid is a target organ for FGF23 in rats. J Clin Invest. 2007, 117: 4003-4008.

    CAS  PubMed  PubMed Central  Google Scholar 

  50. Walther F, Fusch C, Radke M, Beckert S, Findeisen A: Osteoporosis in pediatric patients suffering from chronic inflammatory bowel disease with and without steroid treatment. J Pediatr Gastroenterol Nutr. 2006, 43 (1): 42-51. 10.1097/01.mpg.0000228105.91240.80.

    Article  PubMed  Google Scholar 

  51. Franchimont N, Putzeys V, Collette J, Vermeire S, Rutgeerts P, De Vos M, et al: Rapid improvement of bone metabolism after infliximab treatment in Crohn’s disease. Aliment Pharmacol Ther. 2004, 20 (6): 607-614. 10.1111/j.1365-2036.2004.02152.x.

    Article  CAS  PubMed  Google Scholar 

  52. Ryan BM, Russel MG, Schurgers L, Wichers M, Sijbrandij J, Stockbrugger RW, et al: Effect of antitumour necrosis factor-alpha therapy on bone turnover in patients with active Crohn’s disease: a prospective study. Aliment Pharmacol Ther. 2004, 20 (8): 851-857. 10.1111/j.1365-2036.2004.02097.x.

    Article  CAS  PubMed  Google Scholar 

  53. Ghishan FK, Kiela PR: Advances in the understanding of mineral and bone metabolism in inflammatory bowel diseases. Am J Physiol Gastrointest Liver Physiol. 2011, 300: G191-G201. 10.1152/ajpgi.00496.2010.

    Article  CAS  PubMed  Google Scholar 

  54. Kanis JA, Melton LJ, Christiansen C, Johnston CC, Khaltaev N: The diagnosis of osteoporosis. J Bone Miner Res. 1994, 9: 1137-1141.

    Article  CAS  PubMed  Google Scholar 

  55. Sylvester FA: IBD and skeletal health: children are not small adults!. Inflamm Bowel Dis. 2005, 11: 1020-1023.

    Article  PubMed  Google Scholar 

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Acknowledgement

Authors would like to thank professors Hoda Tomoum and Ola Al-Masry for their kind revision of the final manuscript.

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Authors and Affiliations

  1. Department Pediatrics, Faculty of Medicine, Ain Shams University, Cairo, Egypt

    Mostafa Abdel-Aziz El-Hodhod & Ahmad Mohamed Hamdy

  2. Department Clinical & Chemical Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt

    Amal Ahmed Abbas

  3. Department Radiodiagnosis, Faculty of Medicine, Ain Shams University, Cairo, Egypt

    Sherine George Moftah

  4. Pediatrician, Ministry of Health, Cairo, Egypt

    Alhag Ahmed Mohamed Ramadan

Authors
  1. Mostafa Abdel-Aziz El-Hodhod
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  2. Ahmad Mohamed Hamdy
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  3. Amal Ahmed Abbas
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  4. Sherine George Moftah
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  5. Alhag Ahmed Mohamed Ramadan
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Correspondence to Mostafa Abdel-Aziz El-Hodhod.

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The authors declare that they have no competing interests.

Authors’ contributions

MAE idea of the work, some endoscopies, discussion of results and revision of the manuscript. AMH some endoscopies, recruitment of patients, clinical assessment and therapy, statistical analysis and writing the manuscript. AAA the laboratory workup. SGM the DXA scan. AAMR literature review and assisted in recruitment of patients. All authors read and approved the final manuscript.

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El-Hodhod, M.AA., Hamdy, A.M., Abbas, A.A. et al. Fibroblast growth factor 23 contributes to diminished bone mineral density in childhood inflammatory bowel disease. BMC Gastroenterol 12, 44 (2012). https://doi.org/10.1186/1471-230X-12-44

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Keywords

  • IBD
  • Children
  • FGF23
  • Bone mineral density

BMC Gastroenterology

ISSN: 1471-230X

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