Low bone mass in microscopic colitis
© Lőrinczy et al; licensee BioMed Central Ltd. 2011
Received: 29 June 2010
Accepted: 19 May 2011
Published: 19 May 2011
Microscopic colitis presents with similar symptoms to classic inflammatory bowel diseases. Osteoporosis is a common complication of Crohn's disease but there are no data concerning bone metabolism in microscopic colitis.
The aim of the present study was to evaluate bone density and metabolism in patients with microscopic colitis.
Fourteen patients microscopic colitis were included in the study, and 28 healthy persons and 28 age and gender matched Crohn's disease patients were enrolled as controls. Bone mineral density was measured using dual x-ray absorptiometry at the lumbar spine, femoral neck and the radius. Serum bone formation and bone resorption markers (osteocalcin and beta-crosslaps, respectively) were measured using immunoassays.
Low bone mass was measured in 57.14% patients with microscopic colitis. Bone mineral density at the femoral neck in patients suffering from microscopic colitis and Crohn's disease was lower than in healthy controls (0.852 ± 0.165 and 0.807 ± 0.136 vs. 1.056 ± 0.126 g/cm2; p < 0.01). Bone mineral density at the non-dominant radius was decreased in microscopic colitis patients (0.565 ± 0.093 vs. 0.667 ± 0.072 g/cm2; p < 0.05) but unaffected in Crohn's disease patients (0.672 ± 0.056 g/cm2). Mean beta-crosslaps concentration was higher in microscopic colitis and Crohn's disease patients than controls (417.714 ± 250.37 and 466.071 ± 249.96 vs. 264.75 ± 138.65 pg/ml; p < 0.05). A negative correlation between beta-crosslaps concentration and the femoral and radius t-scores was evident in microscopic colitis patients.
Low bone mass is frequent in microscopic colitis, and alterations to bone metabolism are similar to those present in Crohn's disease. Therefore, microscopic colitis-associated osteopenia could be a significant problem in such patients.
Keywordsbone microscopic colitis Crohn's disease osteoporosis
Microscopic colitis (MC) is defined by chronic, watery diarrhoea, abdominal pain and weight loss. However, macroscopically normal colonic mucosa is evident on radiological and endoscopic examination, and microscopic examination is required for the detection of diagnostic histopathological features [1, 2]. MC normally occurs in middle-aged patients, with a peak incidence in individuals aged approximately 65 years. The annual incidence of MC is between four and six per 100,000 individuals. However, the significance of these morphologically distinct diseases is underestimated in daily clinical practice and the pathogenesis underlying MC has yet to be elucidated. Abnormal collagen metabolism , bacterial toxins  and drugs  could be responsible for mucosal injury in MC, although the colonic mucosa appears normal on colonoscopy examination and diagnosis is established using histology.
Two types of MC were initially described more than 30 years ago [6, 7]. Collagenous colitis (CC) is defined by a sub-epithelial collagen layer wider than 10 μm . Diagnostic criteria for lymphocytic colitis (LC) are more than 10  or 20  intraepithelial lymphocytes (IEL)/100 epithelial cells of the colonic mucosa. Microscopic colitis is thought to be a multifactorial disease but the exact cause is unknown. A high incidence in families suggests a degree of genetic susceptibility [11, 12]. There is a high incidence of spontaneous resolution of symptoms, and budesonide is the drug of choice for first-line treatment in patients with severe symptoms .
A relationship between MC and members of the classic inflammatory bowel disease (IBD) group including Crohn's disease (CD) and ulcerative colitis (UC) is based on epidemiological, pathological and clinical associations. Several case reports demonstrate that MC can progress to IBD [14–16]. However, a large retrospective analysis demonstrated no association between the presence of MC and progression to IBD . Olesen et al. demonstrated that 12% of patients with LC reported a family history of other bowel disorders including IBD, celiac disease and CC .
Decreased bone mineral density (BMD) is a common complication of IBD and is present in 30-77% of cases [18, 19], predominantly in patients suffering from CD . Compared to healthy controls, individuals suffering from CD have a relative risk of vertebral and hip fracture of 1.59 and 1.72, respectively . Recent data indicate that low bone mass associated with CD correlates with the basic pathology of CD rather than malabsorption or complications of steroid treatment [22, 23].
There are currently no studies concerned with possible alterations in bone metabolism in MC patients. The aim of this study was to evaluate the bone density and bone metabolism in patients with MC. The secondary aim was to compare the alterations in bone metabolism of MC patients with bone metabolism in CD patients and healthy controls.
Fourteen MC patients (12 women and two men with a mean age of 49.79 ± 13.06 years) were included in the study. Ten were diagnosed with LC and four with CC. Enrolment criteria included asymptomatic patients who had not taken medication for six weeks prior to commencement of the study. Those patients who had been subjected to treatment with budesonide for longer than eight weeks, or within six weeks prior to enrolment in the study, were excluded from participating. The short plasma half-life of budesonide (1.5-3.5 hours) , the 24 hours duration of effects and the first pass effects of the drug were taken into consideration to define the wash-out period. Remission during MC was defined as two or less bowel movements per day without taking medication. Remission had been achieved using budesonide during the history of these patients; none of the enrolled patients had been previously treated with systemic steroids. The presence of celiac disease was excluded using serology (tissue transglutaminase and endomysial antibody) and duodenal histology. Lymphocytic colitis was defined as more than 10 IELs/100 epithelial cells situated in the mucosa, and the diagnostic criterion for CC was a subepithelial collagen layer wider than 10 μm.
Twenty-eight healthy persons (HC) and 28 CD patients matched for age, gender and postmenopausal state were enrolled as controls. CD patients were in remission as defined by the Crohn's disease activity index (CDAI < 150) , and had been steroid free for one year. Azathioprine up to 2.5 mg/body weight kg and mesalazine up to 4 g daily was permitted as a maintenance therapy for CD patients. None of the participants had the stricturing or penetrating (Vienna Classification B2 or B3) types of the disease . The members of the HC group were matched for age, gender and menopausal status to the MC patients. Subjects provided written informed consent, and the study protocol was approved by the regional and institutional committee of science and research ethics (SE-106/2007).
Demographical data, significant medical history (localization and duration of disease, surgical history and drug use), risk factors for osteoporosis (body mass index, menopausal state, smoking status, family history for osteoporosis and previous low trauma bone fracture) were recorded. Patients taking medications that affected vitamin K metabolism were not eligible to participate in the study.
Bone mineral density measurements
Bone mineral density (BMD) measurements were performed using dual-energy X-ray absorptiometry of the lumbar spine (L2-L4), the left femoral neck and the non-dominant radius using a Hologic QDR 4500C instrument (Hologic, Waltham, MA). For analysis, v. 9.03D software was used. Z-scores were calculated according to the manufacturer's reference curves (the number of standard deviations (SD) from age- and sex-matched healthy controls). The third National Health and Nutrition Examination Surveys (NHANES III) normative data were used as a reference database for femoral bone density measurements. World Health Organization (WHO) criteria for low BMD were applied for this analysis . Low bone mass and osteoporosis were defined as a BMD t-score below -1 and below -2.5, at the lumbar spine or the femoral neck, respectively. Quality control was maintained by carrying out daily scanning of an anthropometric spine phantom. The coefficient for the variation of BMD measurements on the spine phantom over a period of four years was 0.35%.
Parameters of bone metabolism
Serum calcium (normal: 2.25-2.61 mmol/l), parathyroid hormone (normal: 10-65 pg/ml) and thyroid stimulating hormone (normal: 0.3-3.3 mU/L) levels were determined before the study commenced to exclude the presence of other types of metabolic bone diseases.
Fasting blood samples for evaluating markers of bone metabolism were taken between 7 and 8 a.m. Osteocalcin (OC) is a bone-specific calcium binding protein produced by osteoblasts during bone synthesis, and serves as a good marker for bone formation. After release, it accumulates in the bone matrix and proportional amounts leak into the blood stream . More than 90% of the organic bone matrix consists of type I collagen. During certain physiological and pathological processes, a degradation product of mature type I collagen, beta-crosslaps (bCL), is released into the bloodstream. Accordingly, bCL is a useful marker for monitoring the process of bone resorption .
Serum OC and bCL levels were measured using an electrochemiluminescence immunoassay (Elecsys N-MID Osteocalcin and Elecsys b-CrossLaps, Roche, Nutley, NJ). The OC immunoassay detects a stable 43 amino acid fragment of the N-terminal end (N-MID fragment). The immunoassay was based on monoclonal antibodies against epitopes located on the stable fragment. The normal concentrations in the serum were 0-320 pg/ml for bCL and 20-48 ng/ml for OC.
Calculations were performed using SPSS statistics 15.0 software. Paired and independent sample Student's t-tests, Pearson correlations and chi-square tests were applied. The results were presented as mean ± SD. Results were considered significant when p < 0.05.
Clinical characteristics of patient groups.
(10 LC and 2 CC)
duration of disease (years)
4.33 ± 1.66
5.03 ± 3.53
actual symptoms (number of liquid stools/day)
1.41 ± 0.66
1.37 ± 0.49
budesonide in the past
p < 0.01
systemic steroid ever
Bone density parameters
Major objective bone density parameters in microscopic colitis, Crohn's disease and healthy controls.
(MC vs. CD)
Femoral BMD (g/cm2)
0.852 ± 0.165**
0.807 ± 0.136**
1.056 ± 0.126
Lumbar BMD (g/cm2)
0.928 ± 0.156
p < 0.05
0.847 ± 0.112*
0.949 ± 0.112
Non-dominant radius BMD (g/cm2)
0.565 ± 0.093*
0.672 ± 0.056*
0.667 ± 0.072
-0.638 ± 1.437*
-0.607 ± 1.09
-1.203 ± 1.42
-1.390 ± 1.124
-1.328 ± 1.041
-1.37 ± 1.135*
-1.090 ± 1.236*
-0.882 ± 1.106
Incidence of osteopenia and osteoporosis in microscopic colitis, Crohn's disease and healthy controls.
(MC vs. CD)
incidence of low bone mass
Bone metabolism parameters
Risk factors for low bone mass
The serum calcium concentrations of the MC, CD and HC groups (2.36 ± 0.08 mg/ml, 2.42 ± 0.11 mg/ml and 2.39 ± 0.09 mg/ml) remained unchanged, and the parathyroid hormone level was in the normal range in patients with MC (36.64 pg/ml).
There are no available data regarding alterations in bone metabolism as a complication of MC. The results presented herein suggest decreased bone density in a cohort of MC patients with no clinical signs of malabsorption.
Bone mass was decreased to the same extent in the MC group and the CD group that had been matched for age, gender and menopausal status. Considering the similarly decreased BMD ratios in the MC and CD groups (57% vs. 46% respectively), and taking into consideration that the incidence of MC is underestimated in daily clinical practice, it is proposed that low bone mass is a common occurrence in patients with undiagnosed MC who suffer from chronic diarrhoea.
Bone density parameters of patients with MC were independent from coexisting risk factors including previous short term budesonide therapy. Based on these data, a common pathogenic factor is proposed for MC and the associated bone loss.
The pathophysiology of low bone mass in gastrointestinal diseases is different from senile or postmenopausal osteoporosis. Accelerated bone resorption - so-called uncoupling - is responsible for low bone mass in CD . It has been demonstrated that serum markers for bone formation and bone resorption are higher in CD than HC . The ratio of bone resorption and bone formation markers was similar in MC and CD patients. Serum concentrations of bCL were doubled in MC and CD patients compared with the HC group. Compensatory elevation of the bone formation marker OC was more modest in MC and CD patients than the HC group, without exceeding the normal range. There was a negative correlation between the bCL concentration and femoral or radius t-score values in MC patients, demonstrating a pathogenic relationship between DEXA and the laboratory measurements.
Bone mineral content was significantly lower in parts of the skeleton with a high cortical versus trabecular bone ratio in MC patients; BMD was more profoundly decreased in the femoral neck and non-dominant radius than in the lumbar spine. The mineral content of these regions was similar in MC and CD patients in the present study; however, a lower BMD was evident at the lumbar spine of CD patients.
Thyroid and parathyroid hormones can affect the two compartments of bone differently: the loss of cortical bone is characteristic of hyperthyroidism and hyperparathyroidism. Normal levels of thyroid stimulating hormone, free thyroid hormones, serum calcium and parathyroid hormones exclude a role for these factors in cortical bone loss of the patients in this study.
No data are available to describe the various regulation of bone homeostasis in the cortical and trabecular bones of IBD patients. However, it is known that factors including sex hormones  and their receptors  have different impacts on cortical and trabecular bones. Hypogonadism is a complication of inflammatory bowel disease, but a pathogenic role for gonad dysfunction has not been proven in regard to the altered bone metabolism in IBD [36, 37].
A major limitation of this study is the low number of MC patients; there was no possibility of performing subgroup analysis in this small cohort. Data concerning LC and CC patients were analyzed together as histology is the only relevant difference between these two types of MC. Furthermore, the mean age of patients with MC is similar to the perimenopausal age and to the first postmenopausal decade; therefore, low BMD in patients with chronic diarrhoea is not a specific indicator of definitive colonic disease. It is suggested that patients with chronic diarrhoea undergo a biopsy during colonoscopy; a specific request to the pathologist should be made to consider the diagnosis of MC. Densitometry should only be performed on patients with histologically confirmed MC.
The present study demonstrates that bone loss can be an important problem in MC. A similarly decreased BMD was observed in patients with MC and CD. Low bone mass was detected in the femur and radius, and these bones contain more cortical than trabecular bone. Uncoupled bone remodelling was demonstrated in MC, with bone resorption demonstrated to exceed compensatory bone formation. The current findings are similar to the changes observed in bone homeostasis in CD.
We thank R. Szmola MD for providing critical professional revision of the manuscript.
- Tysk C, Bohr J, Nyhlin N, Wickbom A, Eriksson S: Diagnosis and management of microscopic colitis. World J Gastroenterol. 2008, 14: 7280-7288. 10.3748/wjg.14.7280.View ArticlePubMedPubMed CentralGoogle Scholar
- Tangri V, Chande N: Microscopic colitis: an update. J Clin Gastroenterol. 2009, 43: 293-296. 10.1097/MCG.0b013e31818f50ce.View ArticlePubMedGoogle Scholar
- Aigner T, Neureiter D, Muller S, Kuspert G, Belke J, Kirchner T: Extracellular matrix composition and gene expression in collagenous colitis. Gastroenterology. 1997, 113: 136-143. 10.1016/S0016-5085(97)70088-X.View ArticlePubMedGoogle Scholar
- Bohr J, Nordfelth R, Jarnerot G, Tysk C: Yersinia species in collagenous colitis: a serologic study. Scand J Gastroenterol. 2002, 37: 711-714. 10.1080/00365520212509.View ArticlePubMedGoogle Scholar
- Giardiello FM, Hansen FC, Lazenby AJ, Hellman DB, Milligan FD, Bayless TM, Yardley JH: Collagenous colitis in setting of nonsteroidal antiinflammatory drugs and antibiotics. Dig Dis Sci. 1990, 35: 257-260. 10.1007/BF01536772.View ArticlePubMedGoogle Scholar
- Lindstrom CG: 'Collagenous colitis' with watery diarrhoea--a new entity?. Pathol Eur. 1976, 11: 87-89.PubMedGoogle Scholar
- Lazenby AJ, Yardley JH, Giardiello FM, Jessurun J, Bayless TM: Lymphocytic ("microscopic") colitis: a comparative histopathologic study with particular reference to collagenous colitis. Hum Pathol. 1989, 20: 18-28. 10.1016/0046-8177(89)90198-6.View ArticlePubMedGoogle Scholar
- Lee E, Schiller LR, Vendrell D, Santa Ana CA, Fordtran JS: Subepithelial collagen table thickness in colon specimens from patients with microscopic colitis and collagenous colitis. Gastroenterology. 1992, 103: 1790-1796.View ArticlePubMedGoogle Scholar
- Kao KT, Pedraza BA, McClune AC, Rios DA, Mao YQ, Zuch RH, Kanter MH, Wirio S, Conteas CN: Microscopic colitis: a large retrospective analysis from a health maintenance organization experience. World J Gastroenterol. 2009, 15: 3122-3127. 10.3748/wjg.15.3122.View ArticlePubMedPubMed CentralGoogle Scholar
- Chatelain D, Mokrani N, Flejou JF: [Microscopic colitis: collagenous colitis and lymphocytic colitis]. Ann Pathol. 2007, 27: 448-458. 10.1016/S0242-6498(07)71417-7.View ArticlePubMedGoogle Scholar
- van Tilburg AJ, Lam HG, Seldenrijk CA, Stel HV, Blok P, Dekker W, Meuwissen SG: Familial occurrence of collagenous colitis. A report of two families. J Clin Gastroenterol. 1990, 12: 279-285. 10.1097/00004836-199006000-00009.View ArticlePubMedGoogle Scholar
- Jarnerot G, Hertervig E, Granno C, Thorhallsson E, Eriksson S, Tysk C, Hansson I, Bjorknas H, Bohr J, Olesen M, et al: Familial occurrence of microscopic colitis: a report on five families. Scand J Gastroenterol. 2001, 36: 959-962.View ArticlePubMedGoogle Scholar
- Chande N, McDonald JW, Macdonald JK: Interventions for treating lymphocytic colitis. Cochrane Database Syst Rev. 2007, CD006096-Google Scholar
- O'Beirne JP, Ireland A: Progression of collagenous colitis to Crohn's disease. Eur J Gastroenterol Hepatol. 2005, 17: 573-575. 10.1097/00042737-200505000-00015.View ArticlePubMedGoogle Scholar
- Janczewska I, Stal P, Sandstedt B: [Transformation of microscopic colitis to inflammatory bowel disease]. Lakartidningen. 2007, 104: 1597-1598.PubMedGoogle Scholar
- Aqel B, Bishop M, Krishna M, Cangemi J: Collagenous colitis evolving into ulcerative colitis: a case report and review of the literature. Dig Dis Sci. 2003, 48: 2323-2327.View ArticlePubMedGoogle Scholar
- Olesen M, Eriksson S, Bohr J, Jarnerot G, Tysk C: Lymphocytic colitis: a retrospective clinical study of 199 Swedish patients. Gut. 2004, 53: 536-541. 10.1136/gut.2003.023440.View ArticlePubMedPubMed CentralGoogle Scholar
- Compston JE, Judd D, Crawley EO, Evans WD, Evans C, Church HA, Reid EM, Rhodes J: Osteoporosis in patients with inflammatory bowel disease. Gut. 1987, 28: 410-415. 10.1136/gut.28.4.410.View ArticlePubMedPubMed CentralGoogle Scholar
- Szathmari M, Pronai L, Tulassay Z: Altered bone metabolism in inflammatory bowel disease. Am J Gastroenterol. 1998, 93: 848-849.View ArticlePubMedGoogle Scholar
- Ardizzone S, Bollani S, Bettica P, Bevilacqua M, Molteni P, Bianchi Porro G: Altered bone metabolism in inflammatory bowel disease: there is a difference between Crohn's disease and ulcerative colitis. J Intern Med. 2000, 247: 63-70. 10.1046/j.1365-2796.2000.00582.x.View ArticlePubMedGoogle Scholar
- van Staa TP, Cooper C, Brusse LS, Leufkens H, Javaid MK, Arden NK: Inflammatory bowel disease and the risk of fracture. Gastroenterology. 2003, 125: 1591-1597. 10.1053/j.gastro.2003.09.027.View ArticlePubMedGoogle Scholar
- Moschen AR, Kaser A, Enrich B, Ludwiczek O, Gabriel M, Obrist P, Wolf AM, Tilg H: The RANKL/OPG system is activated in inflammatory bowel disease and relates to the state of bone loss. Gut. 2005, 54: 479-487. 10.1136/gut.2004.044370.View ArticlePubMedPubMed CentralGoogle Scholar
- Miheller P, Muzes G, Zagoni T, Toth M, Racz K, Tulassay Z: Infliximab therapy improves the bone metabolism in fistulizing Crohn's disease. Dig Dis. 2006, 24: 201-206. 10.1159/000091299.View ArticlePubMedGoogle Scholar
- Dilger K, Alberer M, Busch A, Enninger A, Behrens R, Koletzko S, Stern M, Beckmann C, Gleiter CH: Pharmacokinetics and pharmacodynamic action of budesonide in children with Crohn's disease. Aliment Pharmacol Ther. 2006, 23: 387-396. 10.1111/j.1365-2036.2006.02771.x.View ArticlePubMedGoogle Scholar
- Best WR, Becktel JM, Singleton JW, Kern F: Development of a Crohn's disease activity index. National Cooperative Crohn's Disease Study. Gastroenterology. 1976, 70: 439-444.PubMedGoogle Scholar
- Gasche C, Scholmerich J, Brynskov J, D'Haens G, Hanauer SB, Irvine EJ, Jewell DP, Rachmilewitz D, Sachar DB, Sandborn WJ, et al: A simple classification of Crohn's disease: report of the Working Party for the World Congresses of Gastroenterology, Vienna 1998. Inflamm Bowel Dis. 2000, 6: 8-15.View ArticlePubMedGoogle Scholar
- World Health Organization. Assessment of Fracture Risk and Application to Screening for Postmenopausal Osteoporosis. 1994, Geneva, Switzerland: World Health OrganizationGoogle Scholar
- Lian JB, Gundberg CM: Osteocalcin. Biochemical considerations and clinical applications. Clin Orthop Relat Res. 1988, 226: 267-291.PubMedGoogle Scholar
- Okabe R, Nakatsuka K, Inaba M, Miki T, Naka H, Masaki H, Moriguchi A, Nishizawa Y: Clinical evaluation of the Elecsys beta-CrossLaps serum assay, a new assay for degradation products of type I collagen C-tlopeptides. Clin Chem. 2001, 47: 1410-1414.PubMedGoogle Scholar
- Bernstein CN, Leslie WD, Leboff MS: AGA technical review on osteoporosis in gastrointestinal diseases. Gastroenterology. 2003, 124: 795-841. 10.1053/gast.2003.50106.View ArticlePubMedGoogle Scholar
- Bernstein CN KS: Guidelines for osteoporosis and inflammatory bowel disease. Aguide to diagnosis and management for the gastroenterologist (monograph). The American College of Gastroenterology. 2003Google Scholar
- Miheller P, Muzes G, Racz K, Blazovits A, Lakatos P, Herszenyi L, Tulassay Z: Changes of OPG and RANKL concentrations in Crohn's disease after infliximab therapy. Inflamm Bowel Dis. 2007, 13: 1379-1384. 10.1002/ibd.20234.View ArticlePubMedGoogle Scholar
- Miheller P, Toth M, Molnar E, Zagoni T, Racz K, Tulassay Z: [Serum bone marker measurements in bone metabolism disorders associated with inflammatory bowel diseases]. Orv Hetil. 2001, 142: 1557-1560.PubMedGoogle Scholar
- Windahl SH, Vidal O, Andersson G, Gustafsson JA, Ohlsson C: Increased cortical bone mineral content but unchanged trabecular bone mineral density in female ERbeta(-/-) mice. J Clin Invest. 1999, 104: 895-901. 10.1172/JCI6730.View ArticlePubMedPubMed CentralGoogle Scholar
- Vico L, Vanacker JM: Sex hormones and their receptors in bone homeostasis: insights from genetically modified mouse models. Osteoporos Int. 2009, 1: 365-72.Google Scholar
- Klaus J, Reinshagen M, Adler G, Boehm B, von Tirpitz C: Bones and Crohn's: estradiol deficiency in men with Crohn's disease is not associated with reduced bone mineral density. BMC Gastroenterol. 2008, 8: 48-10.1186/1471-230X-8-48.View ArticlePubMedPubMed CentralGoogle Scholar
- Robinson RJ, Iqbal SJ, Al-Azzawi F, Abrams K, Mayberry JF: Sex hormone status and bone metabolism in men with Crohn's disease. Aliment Pharmacol Ther. 1998, 12: 21-25. 10.1046/j.1365-2036.1998.00271.x.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-230X/11/58/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.