- Case report
- Open Access
Ultrasound contrast agent Sonazoid for the diagnosis of hepatic epithelioid angiomyolipoma: a case report
BMC Gastroenterology volume 21, Article number: 487 (2021)
An angiomyolipoma usually occurs in the kidneys and rarely in the liver. Hepatic epithelioid angiomyolipoma (HEAML), a rare variant of angiomyolipoma, possesses malignant potential and mimics the imaging features of hepatocellular carcinoma. Sonazoid® (perfluorobutane microbubbles), a new contrast agent that facilitates hepatic parenchyma-specific Kupffer phase imaging on contrast-enhanced ultrasonography (CEUS), is useful for the detection and characterization of focal liver lesions.
A 30-year-old man with HEAML underwent CEUS using Sonazoid®, in which new concepts for ultrasonography-based differentiation between HEAML and hepatocellular carcinoma were applied.
This case report provides clinicians and radiologists with a reference for distinguishing HEAML from hepatocellular carcinoma based on CEUS using Sonazoid®.
Hepatic epithelioid angiomyolipoma (HEAML) is a rare variant of angiomyolipoma. Angiomyolipoma is a stromal tumor composed of smooth muscle cells, varying amounts of adipose tissue, and thick-walled blood vessels . HEAML is mainly composed of epithelioid cells that possess malignant potential . It can show striking cytologic atypia and multinucleation and may have local recurrence and metastasis . Further, it can mimic the imaging features of hepatocellular carcinoma (HCC), such as hypo-enhancement in the portal phase in contrast-enhanced computed tomography (CT) , and therefore, it is difficult to clearly diagnose HEAML before surgery. The diagnosis and differential diagnosis of HEAML are very important owing to the differences in their respective prognoses. However, specific clinical manifestations and reliable laboratory test evidence for HEAML are lacking. Contrast-enhanced ultrasonography (CEUS) performed using a blood-pool contrast agent has demonstrated high sensitivity and specificity for characterizing focal liver lesions . Sonazoid® (Daiichi-Sankyo, Tokyo, Japan; GE Healthcare, Milwaukee, WI, USA), a new contrast agent that permits hepatic parenchyma-specific Kupffer phase imaging on CEUS, leads to the observation of a persistent enhancement during the postvascular phase (Kupffer phase) for > 10 min after injection . It is very useful for the detection and characterization of focal liver lesions and is now available in Japan, South Korea, Norway, and Taiwan .
This case study aimed to analyze the CEUS characteristics of HEAML using the Sonazoid contrast agent, and to devise new concepts to discrimination between HEAML and HCC.
Physical examination of a 30-year-old man revealed hepatic space-occupying lesions, in the absence of chills, fever, nausea, vomiting, jaundice, and other symptoms. He was referred to our hospital for further treatment after a few days.
Informed consent was obtained from the patient prior to the examination. The patient underwent CEUS at our inpatient department with a LOGIC E9 ultrasound scanner (GE Healthcare, Milwaukee, WI, USA). The level of low mechanical index (MI) in CEUS was 0.2. B-mode imaging revealed a hypoechoic zone (4.6 cm × 3.6 cm) in the caudate lobe of the liver, with a clear boundary, uneven internal echo, and hypoechoic zone surrounded by a hyperechoic ring. Color Doppler flow imaging did not find any obvious blood flow signal in the above-mentioned hypoechoic zone. CEUS revealed that the lesion was filled with the contrast agent, its center was moderately enhancing, and the peripheral area was hyperenhancing in the arterial phase. Contrast agent wash-out could be observed as a hypoenhancement at the center of the lesion and the peripheral hyperechoic area remained as a hyperenhancement in the portal venous phase. The central area exhibited further wash-out in the late vascular phase. The lesion showed a defect in the Kupffer phase (Fig. 1). Quantitative CEUS parameters reflecting tissue vascularity can be obtained from time-intensity curve (TIC) analysis. The fitted TIC of a region of interest can be obtained on a pixel-by-pixel basis using SonoLiver (TomTec, Germany, and Bracco, Italy). Quantitative analysis of CEUS showed that the contrast arrival time for the lesion was 8 s, the fall time for the lesion was 33 s, the mean transit time for the lesion was 15 s, and the time to peak for the lesion was 24 s. The peak intensity of the lesion was − 56 dB. The intensity of the lesion during the Kupffer phase was − 60 dB. The time to peak for the surrounding liver tissue was 13 s, the peak intensity of the surrounding liver tissue was − 62 dB, and the intensity of the surrounding liver tissue during the Kupffer phase was − 44 dB. The CEUS findings indicated that the liver lesions were benign but failed to clarify their specific nature. The patient underwent enhanced CT on the same day. The enhanced CT images showed a rounded, abnormal enhancement in the caudate lobe of the liver near the second hepatic hilum (4.4 cm × 4.1 cm), with obvious hyper-enhancement in the arterial phase, and wash-out in the portal and delayed phases (Fig. 2). The inferior vena cava and hepatic vein were compressed, and no obvious filling defect was observed, considering the possibility of HCC. During hospitalization, the patient underwent complete laboratory examinations within the next three days. He did not have a current or past history of hepatitis B virus and hepatitis C virus infection. The levels of alpha-fetoprotein (AFP), carcinoembryonic antigen, and sugar chain antigen were 1.72 ng/mL, 0.89 ng/mL, and 19–94.46 U/mL, respectively. The white blood cell count was 4.30 × 109/L. The levels of alanine aminotransferase, aspartate aminotransferase, and total bilirubin were 19 U/L, 16 U/L, and 9.8 µmol/L, respectively.
We decided to perform laparoscopic hepatic caudate lobe resection for this patient after consultation with the hepatobiliary surgeon. Laparoscopic ultrasound exploration conducted during surgery revealed a soft, gray-brown mass measuring 3 cm × 3 cm located on the Spiegel’s lobe near the inferior vena cava. The Spiegel’s lobe was partially excised during surgery. The results of the histopathological examination showed that the lesion was a liver angiomyolipoma, and some areas were composed mainly of epithelioid and spindle-shaped cells (Fig. 2). The findings of immunohistochemical analysis were as follows: HMB45 ( +), smooth muscle actin ( +), cathepsin K ( −), S-100 ( −), SOX10 ( −), hepatocyte ( −), glypican-3 ( −), arginase ( −), AFP (-), GS ( +), cytokeratin (CK)19 ( −), EMA ( −), CK7 ( −), CK20 ( −), CD34 ( −), CD117 (c-kit 9.7) (-), CD117 (positive control) ( +), DOG1 ( −), DES ( −), caldesmon ( −), succinate dehydrogenase B ( +), CC21 ( −), CD23 ( −), CD35 ( −), and Ki-67 (LI approximately 3%). The results of fluorescence in situ hybridization were as follows: Epstein–Barr virus encoded RNA (EBER) chromogenic in-situ hybridization (CISH) ( −) and EBER CISH (positive control) ( −). The patient recovered well and was discharged after the surgery, and no tumor recurrence was observed at the 7-month follow-up.
Discussion and conclusions
Hepatic angiomyolipoma is a rare hepatic stromal tumor, and HEAML is among its rare variants. Only a few more than 409 cases of EAML have been reported to date . EAML is mainly composed of epithelioid cells and has a malignant tendency . The differential diagnosis of HEAML and HCC is critical because of the differences in their treatment strategies and prognoses. The diagnosis of HEAML is often challenging because imaging alone cannot differentiate between HCC and HEAML in several instances.
HEAML mainly presents with hypoechoic nodules on gray-scale ultrasonography similar to HCC; this is consistent with our previous research . The hyperechoic zone surrounding the HEAML lesion on gray-scale ultrasonography in this case may be a manifestation of the fibrosis of the liver parenchyma caused by long-term compression by the tumor. This has never been reported previously.
Hyperenhancement of the peripheral area in the arterial phase demonstrated by CEUS may be due to fibrosis of the liver parenchyma caused by long-term compression by the tumor. The deposition of extracellular matrix of liver fibrosis and the formation of hepatic sinusoidal capillaries lead to a state of high blood flow circulation. CEUS of HEAML showed rapid wash-out that is consistent with the performance of enhanced CT. Tumor cells have reduced interstitium, a rich vascular network, relatively thin tube walls, and increased blood flow, resulting in faster diffusion of the contrast agent, while the portal venous phase and late vascular phase are relatively lower than the liver parenchymal signal. The case study also used quantitative analysis software to assess the utility of Sonazoid in imaging HEAML and found that its performance was consistent with that of SonoVue in our previous study on HEAML . The majority of the clinical evidence for the diagnosis of focal liver lesions with CEUS was obtained with SonoVue, while little is known about Sonazoid. It may be necessary to use this reagent for further studies on HEAML owing to the scarcity of published studies that have accurately correlated the enhancement mode and diagnostic accuracy of Sonazoid. Sonazoid has an additional Kupffer phase on hepatic CEUS. HEAML and HCC both exhibit hypo-enhancement in the Kupffer phase. In our case study, the enhancement intensity of HEAML in the Kupffer phase was − 62 dB. However, we studied 60 HCC lesions and found that the average enhancement intensity in the Kupffer phase was − 54 dB. The difference in the enhancement intensity of the HEAML tumor and surrounding liver tissue in the Kupffer phase was 17 dB, and that between HCC and the surrounding liver tissue was 6 dB. The difference between HEAML and HCC in the Kupffer phase and hyperenhancement of the HEAML peripheral area in the arterial phase may be their distinguishing factor. This requires more research of HEAML cases for verification.
Immunohistochemistry can help in differentiating HEAML from HCC. HEAML tumor cells express melanocyte markers (HMB45) and SMA to varying degrees, while epithelial cell markers (Pan-CK, EMA) are usually negative, which can help identify true epithelial tumors [10, 11].
This case report provides clinicians and radiologists with a reference basis for Sonazoid-based CEUS for HEAML. Future prospectively designed studies on the use of Sonazoid for HEAML cases should be done, which will help verify the characteristics of its differential diagnosis.
Availability of data and materials
This case report contains clinical data from the electronic medical record in the Nanjing Drum Tower Hospital. The datasets used during the current study are available from the corresponding author on reasonable request.
Hepatic epithelioid angiomyolipoma
Epstein–Barr virus encoded RNA
Chromogenic in-situ hybridization
Nonomura A, Mizukami Y, Kadoya M. Angiomyolipoma of the liver: a collective review. J Gastroenterol. 1994;29(1):95–105.
Tan Y, Xie XY, Li XJ, Liu DH, Zhou LY, Zhang XE, Lin Y, Wang W, Wu SS, Liu J, et al. Comparison of hepatic epithelioid angiomyolipoma and non-hepatitis B, non-hepatitis C hepatocellular carcinoma on contrast-enhanced ultrasound. Diagn Interv Imaging. 2020;101(11):733–8.
Thway K, Fisher C. PEComa: morphology and genetics of a complex tumor family. Ann Diagn Pathol. 2015;19(5):359–68.
Jeon TY, Kim SH, Lim HK, Lee WJ. Assessment of triple-phase CT findings for the differentiation of fat-deficient hepatic angiomyolipoma from hepatocellular carcinoma in non-cirrhotic liver. Eur J Radiol. 2010;73(3):601–6.
Ye J, Xie X, Liu B, Zhang X, Wang W, Huang X, Lu M, Huang G. Imaging features on contrast-enhanced ultrasound and clinical characteristics of hepatitis B virus-related combined hepatocellular-cholangiocarcinoma: comparison with hepatitis B virus-related hepatocellular carcinoma. Ultrasound Med Biol. 2017;43(11):2530–6.
Huang Z, Wu X, Li S, Li K. Contrast-Enhanced Ultrasound Findings And Differential Diagnosis Of Hepatic Epithelioid Angiomyolipoma Compared With Hepatocellular Carcinoma. Ultrasound Med Biol. 2020;46(6):1403–11.
Moriyasu F, Itoh K. Efficacy of perflubutane microbubble-enhanced ultrasound in the characterization and detection of focal liver lesions: phase 3 multicenter clinical trial. AJR Am J Roentgenol. 2009;193(1):86–95.
Mao J, Teng F, Yuan H, Ni Z, Hong Fu, Liu C, Sun K, Zou Y, Dong J, Dong J, et al. 409 patients with hepatic epithelioid angiomyolipoma: a pooled analysis. Chin J Hepatobiliary Surg. 2018;24(10):659–63.
Klompenhouwer AJ, Verver D, Janki S, Bramer WM, Doukas M, Dwarkasing RS, de Man RA. IJzermans J: Management of hepatic angiomyolipoma: a systematic review. Liver Int. 2017;37(9):1272–80.
O’Malley ME, Chawla TP, Lavelle LP, Cleary S, Fischer S. Primary perivascular epithelioid cell tumors of the liver: CT/MRI findings and clinical outcomes. Abdom Radiol (NY). 2017;42(6):1705–12.
Nguyen T, Terris B, Barat M. Hepatic epithelioid angiomyolipoma mimicking hepatocellular carcinoma. Diagn Interv Imaging. 2020;101(7–8):501–3.
There is no funding in this article.
Ethics approval and consent to participate
Authors’ institution does not require ethical approval for publication of a single case report. Written informed consent was obtained from the patient.
Consent for publication
Written informed consent was obtained from the patient for publication of this case report and the accompanying images.
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Huang, Z., Xin, Jy. & Li, Ky. Ultrasound contrast agent Sonazoid for the diagnosis of hepatic epithelioid angiomyolipoma: a case report. BMC Gastroenterol 21, 487 (2021). https://doi.org/10.1186/s12876-021-02064-1
- Hepatic epithelioid angiomyolipoma
- Hepatocellular carcinoma
- Case report