Table 1 The primers used in this study
Target gene description | Primer | Sequence 5′ → 3' | Amplicon size (bp) | Annealing temperature | References |
|---|---|---|---|---|---|
Universal DNA bacterial | 16s-rRNA-F16s-rRNA-R | 5-AGGAGGTGATCCAACCGCA-35-ACCTGGAGGAAGGTGGGGAT-3 | 367 | 55 | [8] |
Salmonella spp. | invA-FinvA-R | 5- TTGTTACGGCTATTTTGACCA-35- CTGACTGCTACCTTGCTGATG-3 | 521 | 60 |  [14] |
Fluoroquinolone | gyrA-FgyrA-R | 5-AAATCTGCCCGTGTCGTTGGT-35-GCCATACCTACGGCGATACC -3 | 344 | 55 |  [7] |
|  | parC-FparC-R | 5-CTGAATGCCAGCGCCAAATT-35-GCGAACGATTTCGGATCGTC-3 | 168 | 55 |  [7] |
|  | qnrS-FqnrS-R | 5-TGGAAACCTACAATCATACATATCG-3 5-TTAGTCAGGATAAACAACAATACCC-3 | 656 | 60 |  [7] |
|  | qnrA-FqnrA-R | 5-GATAAAGTTTTTCAGCAAGAGG-35-ATCCAGATCGGCAAAGGTTA-3 | 593 | 60 |  [7] |
|  | qnrB-FqnrB-R | 5-GTTGGCGAAAAAATTGACAGAA-35-ACTCCGAATTGGTCAGATCG-3 | 264 | 53 |  [7] |
Integrase1 | Int1-FInt1-R | 5-CAGTGGACATAAGCCTGTTC-35-CCCGAGGCATAGACTGTA-3 | 160 | 55 | [7] |
Integrase2 | Int2-FInt2-R | 5-TTGCGAGTATCCATAACCTG-35-TTACCTGCACTGGATTAAGC-3 | 288 | 55 |  [7] |
Integrase3 | Int3-FInt3-R | 5-GCCTCCGGCAGCGACTTTCAG-35-ACGGATCTGCCAAACCTGACT-3 | 979 | 59 | [7] |
Sulfonamide resistance | Sul1-FSul1-R | 5-TCACCGAGGACTCCTTCTTC-35-CAGTCCGCCTCAGCAATATC-3 | 331 | 65 |  [7] |
|  | Sul2-FSul2-R | 5-CCTGTTTCGTCCGACACAGA-35-GAAGCGCAGCCGCAATTCAT-3 | 435 | 58 |  [7] |
Quaternary ammonium compounds | qac-Fqac-R | 5-GCCCTACACAAATTGGGAGA-35-CTGCGGTACCACTGCCACAA-3 | 370 | 55 | [15] |