Figure 6
From: Activation of Liver FGF21 in hepatocarcinogenesis and during hepatic stress
Upregulation of FGF21 expression in human hepatic tissues during HCC development. The expression levels of FGF21 were assessed by IHC (cytosolic maroon or orange stains) with anti-FGF21 antibody in the liver tissue sections from human patients as described in the Methods section. Representative images taken by light microscopy were shown for the following disease conditions: (A) grade 1 HCC tissues and (B) the tumor-adjacent (≥1.5 cm from the edge of tumor foci) hepatic tissues, (C) grade 2 HCC tissues and (D) the tumor-adjacent hepatic tissues, (E) grade 3 HCC tissues and (F) the tumor-adjacent hepatic tissues. This is in contrast to the normal hepatic tissues from healthy individuals (G), and phenotypically normal liver tissues (H) from the HCC patients. Low-magnification (×100) images are on the left, and black-boxed sections are enlarged (×1000) on the right. Green arrowhead: concentrated cytoplasmic FGF21 staining; Yellow arrow: enlarged or irregular nucleus; Blue arrow: fibrotic cirrhotic area.