DPO multiplex PCR as an alternative to culture and susceptibility testing to detect Helicobacter pylori and its resistance to clarithromycin

Table 2 Comparison of Helicobacter pylori susceptibility to clarithromycin by E-test, real-time FRET-PCR and DPO-PCR on human gastric biopsies

E-test	FRET-PCR	DPO-PCR	Total (n = 60)
R	R*	R^$	25
WT	WT	WT	15
WT	R^μ	R	1
WT	WT	R	1
R	WT	NEG	1
ND	R	R	4
ND	WT	WT	11
ND	WT	NEG	2^§

R: macrolide resistant strain; WT: wild type (macrolide susceptible strain); NEG: negative result; ND: not determined.
* 3 biopsies with a mixture of a wild type and A2142G/A2143G mutants.
^μ 1 biopsy with a mixture of a wild type and A2142G/A2143G mutants.
^$ 1 double population A2142G + A2143G.
^§ positive histology.

ISSN: 1471-230X