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Helicobacter pylori infection and gastroduodenal diseases in Vietnam: a cross-sectional, hospital-based study
- Tung L Nguyen†1, 2Email author,
- Tomohisa Uchida†1, 3,
- Yoshiyuki Tsukamoto1,
- Dung T Trinh4,
- Long Ta5,
- Bang H Mai5,
- Song H Le6,
- Ky D Thai5,
- Dung D Ho7,
- Hai H Hoang8,
- Takeshi Matsuhisa9,
- Tadayoshi Okimoto2,
- Masaaki Kodama2,
- Kazunari Murakami2,
- Toshio Fujioka2,
- Yoshio Yamaoka10 and
- Masatsugu Moriyama1
© Nguyen et al; licensee BioMed Central Ltd. 2010
Received: 1 June 2010
Accepted: 30 September 2010
Published: 30 September 2010
The rate of H. pylori infection in Vietnam is reportedly high, but the spectrum of H. pylori-associated gastroduodenal diseases has not been systematically investigated. Moreover, despite the similarities of ethnicity and diet, the age-standardized incidence rate of gastric cancer in the northern city of Hanoi is higher than that in the southern city of Ho Chi Minh, but the reason for this phenomenon is unknown. The virulence of Vietnamese H. pylori has also not been investigated in detail.
Individuals undergoing esophagogastroduodenoscopy were randomly recruited. H. pylori infection status was determined based on the combined results of culture, histology, immunohistochemistry, rapid urine test and serum ELISA. Peptic ulcer (PU) and gastroesophageal reflux disease was diagnosed by endoscopy, and chronic gastritis was determined histologically. H. pylori virulence factors were investigated by PCR and sequencing.
Among the examined patients, 65.6% were infected with H. pylori. The prevalence of infection was significantly higher in those over 40 years of age than in those aged ≤40. Chronic gastritis was present in all H. pylori-infected individuals, 83.1% of whom had active gastritis, and 85.3% and 14.7% had atrophy and intestinal metaplasia, respectively. PU was present in 21% of infected patients, whereas its incidence was very low in non-infected individuals. The prevalence of PU was significantly higher in Hanoi than in Ho Chi Minh. The prevalence of vacA m1, which has been identified as an independent risk factor for PU in Vietnam, was significantly higher among H. pylori isolates from Hanoi than among those from Ho Chi Minh.
H. pylori infection is common in Vietnam and is strongly associated with PU, active gastritis, atrophy and intestinal metaplasia. vacA m1 is associated with an increased risk for PU and might contribute to the difference in the prevalence of PU and gastric cancer between Hanoi and Ho Chi Minh.
Helicobacter pylori (H. pylori) is a spiral, Gram-negative bacterium that chronically infects more than half of the world's population, and is currently recognized to play a causative role in the pathogenesis of gastritis, gastroduodenal ulcer, gastric adenocarcinoma and mucosa-associated lymphoid tissue (MALT) lymphoma [1, 2]. Infection with H. pylori almost always results in chronic gastritis, but more severe diseases such as peptic ulcer and gastric cancer develop in only a small proportion of infected patients, suggesting that the clinical outcomes are probably determined by the interaction of bacterial virulence, host genetic susceptibility and environmental factors [2, 3]. To date, several H. pylori virulence factors associated with severe clinical outcomes have been reported, including cagA, cagE, vacA, babA, oipA, iceA and homB [4–11].
In Vietnam, the rate of H. pylori infection is reportedly high , but the spectrum of H. pylori-associated gastroduodenal diseases has not been investigated systematically. Moreover, despite the similarities of ethnicity and diet, the age-standardized incidence rate (ASR) of gastric cancer in the northern city of Hanoi is about 1.5 times higher than that in the southern city of Ho Chi Minh (27.0 vs. 18.7 cases per 100.000 males and 13.2 vs. 8.1 cases per 100.000 females, respectively) , but the reason for this intriguing phenomenon is unknown. Additionally, the ASR of gastric cancer in Vietnam is approximately 3 times lower than that in Japan and Korea , despite the fact that the prevalence of H. pylori infection in Vietnam is reportedly higher . This phenomenon, regarded as an "Asian enigma", is thought to be partly attributable to geographic variations in bacterial virulence [14, 15]. Nevertheless, the virulence of Vietnamese H. pylori strains has not been extensively investigated. Therefore, we carried out the present cross-sectional study to clarify these unresolved issues.
Characteristics of the study population
No. of participants (%)
Number (%) in region
Ho Chi Minh
Sex, no. (%)
Age group, no. (%)
Mean age (range)
Before endoscopy, participants were interviewed by trained medical staff to ascertain their medical history and lifestyle factors. During endoscopy, 5 biopsy specimens (two from the antrum, two from the corpus and one from the upper part of the lesser curvature) were taken and subsequently used for H. pylori culture and histopathologic examination. Peptic ulcer disease (PU) including gastric ulcer (GU), duodenal ulcer (DU) and gastroduodenal ulcer (GDU), and gastroesophageal reflux disease (GERD) were diagnosed by endoscopic observation, while chronic gastritis was determined histologically. After endoscopy, urine and blood samples from all participants were collected on the same day and tested using the rapid urine test and a serum ELISA kit, respectively.
Determination of H. pylori infection status
To maximize the diagnostic accuracy, 5 different methods were combined for the diagnosis of H. pylori infection, including culture, histology, immunohistochemistry, rapid urine test and serum ELISA.
For H. pylori culture, two biopsy specimens from the antrum and body were homogenized in saline and inoculated onto Mueller Hinton II Agar medium (Becton Dickinson, NJ, USA) supplemented with 7% horse blood without antibiotics. The plates were incubated for up to 10 days at 37°C under microaerophilic conditions (10% O2, 5% CO2 and 85% N2). H. pylori was identified on the basis of colony morphology, gram staining and positive reactions for oxidase, catalase, and urease. Isolated strains were stored at -80°C in Brucella Broth (Difco, NJ, USA) containing 10% dimethylsulfoxide and 10% horse serum.
HE and Giemsa staining, and immunohistochemistry with anti-H. pylori polyclonal antibody (Dako, Denmark) were performed using serial sections of three specimens from the antrum, corpus and upper part of the lesser curvature, as described previously . Results were judged by an experienced pathologist (T.U.) who was unaware of other information about the subjects.
Urine and blood samples were collected and tested using the urine RAPIRUN test (Otsuka Pharmaceutical Co., Ltd., Tokyo, Japan) and E Plate kit (Eiken Chemical Co., Ltd., Tokyo, Japan), respectively, in accordance with the manufacturers' instructions.
H. pylori-positive status was determined by positive culture or, in the case of negative culture, by at least two positive results among the following tests: histology, immunohistochemistry, rapid urine test and serum ELISA. Patients were diagnosed as H. pylori-negative when all five tests gave negative results. If only one test other than culture was positive, patients were considered to have undetermined H. pylori infection status and were excluded from further analysis.
Diagnosis and scoring of chronic gastritis
Biopsy specimens were stained with HE and examined by an experienced pathologist (T.U.). For each biopsy specimen, the grades of neutrophil infiltration, mononuclear cell infiltration, atrophy and intestinal metaplasia were scored on the basis of the updated Sydney System (0, none; 1, mild; 2, moderate; and 3, severe).
Active gastritis was classified into four groups as described previously: (i) non-active gastritis, (ii) antrum-predominant gastritis (AP), (iii) corpus-predominant gastritis (CP) and (iv) pangastritis (PAN) [17, 18].
Investigation of H. pylori virulence factors
H. pylori was subcultured from the stock. Multiple colonies on agar plates were harvested, and genomic DNA was extracted using the standard phenol-chloroform method.
The virulence factors of H. pylori, including cagA, cagE, vacA s/m/i, iceA, babA and homB, were examined using PCR as described previously elsewhere [5, 7, 8, 19–22]. The status of oipA, "on" or "off", was determined by examining its signal sequence, as described previously . Sequencing was performed with a Big Dye Terminator v3.1 Cycle Sequencing Kit on a ABI Prism 310 Genetic Analyzer (Applied Biosystems, CA, USA) in accordance with the manufacturer's instructions. All the primers used in this study were purchased from Sigma-Aldrich Japan.
Chi-squared test, Ficher's exact test, one-way ANOVA test, and multivariate analysis were used. Differences at p < 0.05 were regarded as statistically significant. Data analysis was performed using SPSS statistical software v.16.0 (SPSS Inc., Chicago, USA).
Prevalence of H. pylori infection
Among the 270 participants, 100, 131, 133, 143 and 141 gave positive results by culture, histology, immunohistochemistry, rapid urine test and serum ELISA, respectively. Based on the combined results of these tests, 177 patients (65.6%) were judged to be infected with H. pylori, 93 (34.4%) were H. pylori-negative, and none was considered to have undetermined H. pylori infection status (Table 1). There was no significant difference in the prevalence of infection between females and males (66.0% vs. 65.0%), or between Hanoi and Ho Chi Minh (66.4% vs. 64.7%) (Table 1). The prevalence of infection did not differ significantly among the 10-year age groups, but was significantly higher in persons over 40 years of age than in those aged ≤40 years (71.4% vs. 57.8%, p = 0.021) (Table 1).
H. pylori infection and gastroduodenal diseases
Among the 270 participants, 38 (14.1%) were endoscopically diagnosed as having PU, including 21 DUs, 6 GUs and 11 GDUs (DU/GU ratio, 3.5); gastric cancer and MALT lymphoma were not detected (Table 1). The prevalence of PUD was 37/177 (20.9%) in the H. pylori-infected group but only 1/93 (1.1%) in the non-infected group (Table 1). Multivariate analysis showed that the presence of PU was strongly associated with H. pylori infection (adjusted OR, 27.8; 95% CI, 3.6 - 200; p = 0.001), but not with other factors such as sex, age group, alcohol and coffee consumption, and smoking.
Clinical outcomes among H. pylori-infected patients in Hanoi and Hochiminh
Hanoi (n = 89)
Hochiminh (n = 88)
GERD was diagnosed endoscopically in 9 cases (3.3%) and was not inversely associated with H. pylori infection status (p = 0.282).
H. pylori infection and chronic gastritis
Multivariate analysis showed that H. pylori positivity was strongly associated with active inflammation (adjusted OR, 41.7; 95% CI, 18.9 - 90.9; p < 0.001), chronic inflammation (adjusted OR, 19.2; 95% CI, 5.5 - 66.7; p < 0.001), atrophy (adjusted OR, 4.2; 95% CI, 2.3 - 7.7; p < 0.001) and intestinal metaplasia (adjusted OR, 3.2; 95% CI, 1.1 - 9.8; p = 0.036) in the gastric mucosa. An age of >40 years conferred an increased risk for atrophy (adjusted OR, 3.0; 95% CI, 1.6 - 5.5; p < 0.001) and intestinal metaplasia (adjusted 3.1; 95% CI, 1.2 - 7.9; p = 0.02).
H. pylori virulence factors and peptic ulcer
Virulence factors of H. pylori from Vietnam
(n = 100)
(n = 24)
(n = 76)
vacA m †
Quadruple positivity †
Prevalence of H. pylori virulence factors in Hanoi and Ho Chi Minh
Hanoi (n = 53)
Ho Chi Minh (n = 47)
p (Hanoi vs. Ho Chi Minh)
We found that the prevalence of H. pylori infection in Vietnam was about 66%, being somewhat similar to that in several other Asian countries . In this study, H. pylori infection was proved to be strongly associated with active gastritis, atrophy, intestinal metaplasia, and especially PU (adjusted OR, 27.8; p = 0.001). We did not detect any case of gastric cancer or MALToma, possibly due to their low prevalence and the small sample size. H. pylori is probably a very important cause of PU in Vietnam, as PU was observed in approximately 21% of infected patients, while it was virtually absent in non-infected subjects.
We discovered that the majority of H. pylori isolates from Vietnam possessed cagA, cagE, babA, oipA "on", vacA s1 and vacA i1, while other genes such as vacA m1, homB and iceA1 were less frequent. Among these virulence factors, only vacA m1 was identified as an independent risk factor for PU (adjusted OR, 3.0). Moreover, the co-existence of vacA m1 with cagA, cagE and babA conferred an increased risk for PU, perhaps due to a synergic effect among them. Current evidence indicates that the ASR of gastric cancer in Hanoi is about 1.5 times higher than that in Ho Chi Minh , and this appeared to accord with the differences between the two cities in the prevalences of antrum-predominant gastritis and pangastritis. Moreover, in the present study the prevalence of PU in Hanoi was also proved to be significantly higher. Our results suggest that the higher prevalence of PU in Hanoi might be attributable to the higher prevalence of H. pylori strains carrying vacAm1 and quadruple positivity in Hanoi, in comparison with Ho Chi Minh. Because H. pylori virulence was associated with both PU and gastric cancer, our data, although not direct, are the first to indicate a reason for the difference in the ASR of gastric cancer between these two regions of Vietnam.
Compared with Japan, the ASR of gastric cancer in Vietnam is much lower , and this seems to be reflected by the DU/GU ratio as well as the pattern of chronic gastritis observed in Vietnamese patients. The DU/GU ratio has been regard as a good marker for evaluating the risk of gastric cancer in certain populations because DU patients have low risk of developing gastric cancer whereas the opposite is true for GU patients [18, 23]. The DU/GU ratio in Vietnam is about four times higher than that in Japan, but still much lower than that in India, where the ASR of gastric cancer is very low [13, 23]. Corresponding well to the high DU/GU ratio, the majority of infected Vietnamese have antrum-predominant gastritis (57%) whereas the rate of corpus-predominant gastritis is low (3.4%) in comparison with Japan (14~17%) [17, 18].
Because H. pylori virulence is thought to contribute partly to the geographic variation in the ASR of gastric cancer [14, 15], we compared various virulence factors of Vietnamese strains with those of Japanese ones. From the literature, it is evident that nearly all Japanese H. pylori strains simultaneously carry cagA, cagE, vacAm1/s1/i1, babA and oipA "on" [6, 10, 11, 22, 24], while among Vietnamese strains, this figure is around 45%. Furthermore, the prevalence of homB-positive strains is also higher in Japan than in Vietnam (over 90% vs. 39%) [8, 25]. From these data, it is tempting to speculate that the difference in ASR of gastric cancer is attributable partly to the difference in bacterial virulence between the two countries.
Our study indicates that H. pylori infection is common in Vietnam and is strongly associated with the development of PU, active gastritis, atrophy and intestinal metaplasia. Moreover, H. pylori vacA m1 is associated with an increased risk for PU and might contribute to the difference in the prevalence of PU and gastric cancer between Hanoi and Ho Chi Minh.
This study was supported in part by a Grant-in-Aid for Young Scientists from the Ministry of Education, Science, Sports and Culture of Japan (B, 227903480003)
We thank Mrs. Yoko Kudo and Mr. Tsuyoshi Iwao for their technical assistance.
- Peek RM, Blaser MJ: Helicobacter pylori and gastrointestinal tract adenocarcinomas. Nat Rev Cancer. 2002, 2: 28-37. 10.1038/nrc703.View ArticlePubMedGoogle Scholar
- Suerbaum S, Michetti P: Helicobacter pylori infection. N Engl J Med. 2002, 347: 1175-1186. 10.1056/NEJMra020542.View ArticlePubMedGoogle Scholar
- Fox JG, Wang TC: Inflammation, atrophy, and gastric cancer. J Clin Invest. 2007, 117: 60-69. 10.1172/JCI30111.View ArticlePubMedGoogle Scholar
- Atherton JC, Peek RM, Tham KT, Cover TL, Blaser MJ: Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori. Gastroenterology. 1997, 112: 92-99. 10.1016/S0016-5085(97)70223-3.View ArticlePubMedGoogle Scholar
- Basso D, Zambon CF, Letley DP, Stranges A, Marchet A, Rhead JL, Schiavon S, Guariso G, Ceroti M, Nitti D, et al: Clinical relevance of Helicobacter pylori cagA and vacA gene polymorphisms. Gastroenterology. 2008, 135: 91-99. 10.1053/j.gastro.2008.03.041.View ArticlePubMedGoogle Scholar
- Fukuta K, Azuma T, Ito Y, Suto H, Keida Y, Wakabayashi H, Watanabe A, Kuriyama M: Clinical relevance of cagE gene from Helicobacter pylori strains in Japan. Dig Dis Sci. 2002, 47: 667-674. 10.1023/A:1017949026509.View ArticlePubMedGoogle Scholar
- Oleastro M, Cordeiro R, Ferrand J, Nunes B, Lehours P, Carvalho-Oliveira I, Mendes AI, Penque D, Monteiro L, Megraud F, et al: Evaluation of the clinical significance of homB, a novel candidate marker of Helicobacter pylori strains associated with peptic ulcer disease. J Infect Dis. 2008, 198: 1379-1387. 10.1086/592166.View ArticlePubMedGoogle Scholar
- Oleastro M, Cordeiro R, Menard A, Yamaoka Y, Queiroz D, Megraud F, Monteiro L: Allelic diversity and phylogeny of homB, a novel co-virulence marker of Helicobacter pylori. BMC Microbiol. 2009, 9: 248-10.1186/1471-2180-9-248.View ArticlePubMedPubMed CentralGoogle Scholar
- Peek RM, Thompson SA, Donahue JP, Tham KT, Atherton JC, Blaser MJ, Miller GG: Adherence to gastric epithelial cells induces expression of a Helicobacter pylori gene, iceA, that is associated with clinical outcome. Proc Assoc Am Physicians. 1998, 110: 531-544.PubMedGoogle Scholar
- Yamaoka Y, Kikuchi S, el-Zimaity HM, Gutierrez O, Osato MS, Graham DY: Importance of Helicobacter pylori oipA in clinical presentation, gastric inflammation, and mucosal interleukin 8 production. Gastroenterology. 2002, 123: 414-424. 10.1053/gast.2002.34781.View ArticlePubMedGoogle Scholar
- Yamaoka Y, Ojo O, Fujimoto S, Odenbreit S, Haas R, Gutierrez O, El-Zimaity HM, Reddy R, Arnqvist A, Graham DY: Helicobacter pylori outer membrane proteins and gastroduodenal disease. Gut. 2006, 55: 775-781. 10.1136/gut.2005.083014.View ArticlePubMedPubMed CentralGoogle Scholar
- Parkin DM: The global health burden of infection-associated cancers in the year 2002. Int J Cancer. 2006, 118: 3030-3044. 10.1002/ijc.21731.View ArticlePubMedGoogle Scholar
- Ferlay JBF, Pisani P, et al: GLOBOCAN 2002: cancer incidence, mortality and prevalence worldwide, Version 2.0.IARC CancerBase no 5. 2004, Lyon: IARC Press, [http://www-dep.iarc.fr/]Google Scholar
- Nguyen LT, Uchida T, Murakami K, Fujioka T, Moriyama M: Helicobacter pylori virulence and the diversity of gastric cancer in Asia. J Med Microbiol. 2008, 57: 1445-1453. 10.1099/jmm.0.2008/003160-0.View ArticlePubMedGoogle Scholar
- Yamaoka Y, Kato M, Asaka M: Geographic differences in gastric cancer incidence can be explained by differences between Helicobacter pylori strains. Intern Med. 2008, 47: 1077-1083. 10.2169/internalmedicine.47.0975.View ArticlePubMedPubMed CentralGoogle Scholar
- Nguyen LT, Uchida T, Kuroda A, Tsukamoto Y, Trinh TD, Ta L, Mai HB, Ho DQ, Hoang HH, Vilaichone RK, et al: Evaluation of the anti-East Asian CagA-specific antibody for CagA phenotyping. Clin Vaccine Immunol. 2009, 16: 1687-1692. 10.1128/CVI.00200-09.View ArticlePubMedPubMed CentralGoogle Scholar
- Imagawa S, Yoshihara M, Ito M, Yoshida S, Wada Y, Tatsugami M, Takamura A, Tanaka S, Haruma K, Chayama K: Evaluation of gastric cancer risk using topography of histological gastritis: a large-scaled cross-sectional study. Dig Dis Sci. 2008, 53: 1818-1823. 10.1007/s10620-007-0077-x.View ArticlePubMedGoogle Scholar
- Uemura N, Okamoto S, Yamamoto S, Matsumura N, Yamaguchi S, Yamakido M, Taniyama K, Sasaki N, Schlemper RJ: Helicobacter pylori infection and the development of gastric cancer. N Engl J Med. 2001, 345: 784-789. 10.1056/NEJMoa001999.View ArticlePubMedGoogle Scholar
- Atherton JC, Cover TL, Twells RJ, Morales MR, Hawkey CJ, Blaser MJ: Simple and accurate PCR-based system for typing vacuolating cytotoxin alleles of Helicobacter pylori. J Clin Microbiol. 1999, 37: 2979-2982.PubMedPubMed CentralGoogle Scholar
- Gerhard M, Lehn N, Neumayer N, Boren T, Rad R, Schepp W, Miehlke S, Classen M, Prinz C: Clinical relevance of the Helicobacter pylori gene for blood-group antigen-binding adhesin. Proc Natl Acad Sci USA. 1999, 96: 12778-12783. 10.1073/pnas.96.22.12778.View ArticlePubMedPubMed CentralGoogle Scholar
- Ikenoue T, Maeda S, Ogura K, Akanuma M, Mitsuno Y, Imai Y, Yoshida H, Shiratori Y, Omata M: Determination of Helicobacter pylori virulence by simple gene analysis of the cag pathogenicity island. Clin Diagn Lab Immunol. 2001, 8: 181-186.PubMedPubMed CentralGoogle Scholar
- Yamaoka Y, Kodama T, Gutierrez O, Kim JG, Kashima K, Graham DY: Relationship between Helicobacter pylori iceA, cagA, and vacA status and clinical outcome: studies in four different countries. J Clin Microbiol. 1999, 37: 2274-2279.PubMedPubMed CentralGoogle Scholar
- Chiba T, Seno H, Marusawa H, Wakatsuki Y, Okazaki K: Host factors are important in determining clinical outcomes of Helicobacter pylori infection. J Gastroenterol. 2006, 41: 1-9. 10.1007/s00535-005-1743-4.View ArticlePubMedGoogle Scholar
- Yamaoka Y, Orito E, Mizokami M, Gutierrez O, Saitou N, Kodama T, Osato MS, Kim JG, Ramirez FC, Mahachai V, et al: Helicobacter pylori in North and South America before Columbus. FEBS Lett. 2002, 517: 180-184. 10.1016/S0014-5793(02)02617-0.View ArticlePubMedGoogle Scholar
- Oleastro M, Cordeiro R, Yamaoka Y, Queiroz D, Megraud F, Monteiro L, Menard A: Disease association with two Helicobacter pylori duplicate outer membrane protein genes, homB and homA. Gut Pathog. 2009, 1: 12-10.1186/1757-4749-1-12.View ArticlePubMedPubMed CentralGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-230X/10/114/prepub
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