Fig. 5

JAG2 promotes the proliferation and activation of LX-2 Cells. (A) The correlation of JAG2 expression and fibrosis marker Col1a1 in liver tissues of cirrhotic patients. p = 0.0393 by Spearman’s correlation coefficient test. (B and C) siRNAs interfering JAG2 siJAG2-1 (B) and siJAG2-2 (C) inhibited the proliferation of LX-2 cells treated by TGF-β (5ng/ml). * p < 0.05 and *** p < 0.001 by two-tailed Student’s t-test. (D and E) siJAG2-1 and siJAG2-2 suppressed the mRNA (D) and protein (E) expression of α-SMA and Col1a1 in LX-2 cells treated by TGF-β (5ng/ml). * p < 0.05 and ** p < 0.01 by two-tailed Student’s t-test. (F) Overexpression of JAG2 promoted the proliferation of LX-2 cells treated by TGF-β (5ng/ml). * p < 0.05 and ** p < 0.01 by two-tailed Student’s t-test. (G and H) Overexpressing JAG2 enhanced the mRNA (G) and protein (H) expression of α-SMA and Col1a1 in LX-2 cells treated by TGF-β (5ng/ml). * p < 0.05 and ** p < 0.01 by two-tailed Student’s t-test. The above experiments were performed in triplicate and data are presented as means ± SD.