Fig. 3

miR-541 suppresses the Notch signaling pathway in HSCs. (A) The effects of miR-541 on the luciferase activities of reporters of liver fibrosis-associated signaling pathways including Notch, TGF-β, Hedgehog, Hippo, Wnt, and NF-κB were detected in LX-2 cells. (B and C) Real-time PCR (B) and Western blot analysis (C) were performed to detect the expression of key effectors of Notch signaling, including Hes1, Hes5, Notch1, and JAG2 in LX-2 cells transfected with miR-541 mimic, miR-541 inhibitor, and their corresponding negative control. * P < 0.05 and ** P < 0.01, NS, not significant, two-tailed Student’s t-tests. Experiments were performed in triplicate and data are presented as means ± SD.