Fig. 5

Methylation and transcriptional levels of ZC3H3 in normal, cirrhotic, hepatocellular carcinoma (HCC) livers, and HCC cell lines. A The methylation levels of ZC3H3 in liver samples from control subjects, patients with alcoholic cirrhosis, and patients with HCC due to chronic alcoholism. P-values were calculated using Hotelling’s T-squared test. B The methylation levels of ZC3H3 in liver samples from control subjects, patients with HCV-infected cirrhosis, or HCC. P-values were calculated using Hotelling’s T-squared test. C The methylation levels of ZC3H3 in normal culture hepatocytes and HCC cell lines. P-values were calculated using Hotelling’s T-squared test. D The transcriptional levels of ZC3H3 in normal, chronic hepatitis, cirrhosis, dysplastic nodule, early HCC, and advanced HCC liver samples. P-values were calculated for each disease versus normal liver. Data are expressed as the mean ± standard deviation. Data were analyzed using the GSE60753 and GSE114564 datasets