Fig. 8

Dietary intervention suppresses gene expression markers of hepatocellular senescence in GAN DIO-NASH mice. A Heatmaps illustrating changes in the expression of hepatic senescence-associated candidate genes in GAN DIO-NASH mice fed the GAN diet for 38-78 weeks as compared to chow-fed mice (n = 11-15 per group). B Heatmaps illustrating changes in the expression of hepatic senescence-associated candidate genes in GAN DIO-NASH mice receiving dietary intervention (chow reversal) as compared to the corresponding vehicle GAN DIO-NASH mouse group. Mice were fed the GAN diet for 34 weeks before switching to chow feeding (chow-reversal) for 8, 16 or 24 weeks (n = 14–16 per group). Chow-fed C57BL/6J mice (Chow vehicle) served as normal controls (n = 10). All mice received vehicle (SC, QD, 5 ml/kg) during the intervention phase of the study. Color gradients in heatmaps indicate significantly upregulated (red color) or downregulated (blue color) gene expression (log2-fold change, false discovery rate p < 0.05). Unregulated genes are indicated without color fill (white color)