Fig. 7

Dietary intervention reduces histological markers of hepatocellular senescence in GAN DIO-NASH mice. A Liver histomorphometric quantification of p21, ϒ-H2AX and p21 + ϒ-H2AX positive cells (number of cells per 10,000 DAPI-stained cells). Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 (compared to Chow Vehicle); ^###p < 0.001 compared to corresponding GAN DIO-NASH vehicle control group. Dunnett’s test one-factor linear model. B Representative images of p21, ϒ-H2AX and DAPI co-staining in normal mice (Chow vehicle), vehicle-dosed GAN DIO-NASH mice (DIO-NASH vehicle 24w) and GAN DIO-NASH mice receiving dietary intervention for 24 weeks (DIO-NASH chow reversal 24w). Arrows indicate cells co-expressing p21 and ϒ-H2AX