Fig. 4

Dietary intervention improves quantitative histopathological hallmarks of NASH and fibrosis in GAN DIO-NASH mice. Representative photomicrographs of HE, PSR, galectin-3, α-SMA and Col1a1 stainings following dietary intervention (chow reversal, Chow rev) for 8, 16 and 24 weeks in GAN DIO-NASH mice (scale bar, 100 µm). Mice were fed the GAN diet for 34 weeks before switching to chow feeding (chow-reversal) once daily for 8, 16 or 24 weeks, or continued on the GAN diet for 8, 16 or 24 weeks (n = 14-16 per group). Chow-fed C57BL/6J mice served controls (n = 10). All mice received vehicle (SC, QD, 5 ml/kg) during the intervention phase of the study