Fig. 3

Dietary intervention improves quantitative histological markers of NASH and fibrosis in GAN DIO-NASH mice. A-E Automated GHOST-based histomorphometrics on histopathological scoring-derived variables, including (A) % hepatocytes with lipid droplets; (B) Number of inflammatory foci; (C) Ballooning index; (D) % area of periportal fibrosis; (E) % area of sinusoidal fibrosis. F-J Conventional quantitative image analysis (% area) of (F) Lipids; (G) Galectin-3.; (H) α-smooth muscle actin (α-SMA); (I) Fibrosis (PSR); (J) Fibrosis (collagen-1a1); ***p < 0.001 (compared to chow vehicle); ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 compared to corresponding GAN DIO-NASH mouse vehicle control group. Dunnett’s test one-factor linear model