Fig. 1

Dietary intervention improves metabolic markers in GAN DIO-NASH mice. Mice were fed the GAN diet for 34 weeks before switching to chow feeding (chow-reversal) for 8, 16 or 24 weeks (n = 14-16 per group). Chow-fed C57BL/6J mice (Chow vehicle) served as normal controls (n = 10). All mice received vehicle (SC, QD, 5 ml/kg) during the intervention phase of the study. A Study outline. B Body weight profile and terminal body weight. C Terminal liver weight. D Plasma and liver biochemistry. Plasma ALT; plasma AST; plasma triglycerides; plasma total cholesterol; liver triglycerides; liver total cholesterol. E Plasma cytokine levels of TNF-α, MCP-1 and IP-10. Mean ± SEM. **p < 0.01, ***p < 0.001 compared to chow vehicle; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 compared to corresponding GAN DIO-NASH vehicle control group. Dunnett’s test one-factor linear model