Fig. 5

Association of M1/M2 macrophages in the colonic LP of the T cell transfer colitis model. (A-F) viSNE plots of the LP cells of sham-treated (A–C) and T cell-transferred (D–F) mice. F4/80⁺ (A and D), CD11c⁺ (B and E) and CD206⁺ (C and F). Encircled area indicates F4/80⁺ macrophages. (G and H) M1 (CD11c⁺) and M2 (CD206⁺) macrophages in the colonic LP of sham-treated (G) and T cell-transferred (H) mice. (I–L) Percentage of M1 (I) and M2 (K) macrophages in the total macrophages, and the numbers of M1 (J) and M2 (L) macrophages in the colonic LP of the colitis model. (M and N) The expressions of TNFα (M) and p40 (N) in M1 (CD11c⁺) macrophages in the colonic LP of T cell-transferred mice. (O and P) The expressions of IL-10 in M2 (CD206⁺) macrophages in the colonic LP of sham-treated (O) and T cell-transferred (P) mice. (Q) Percentage of IL-10⁺CD206⁺ cells in the total macrophages in the colonic LP of the colitis model. Results are expressed as the mean ± SD. **p < 0.01 via Student’s t-test vs. sham-treated group. n = 4 animals per group. (R–U) Expression levels of M1 markers in isolated macrophages of the colonic LP of sham-treated and T cell-transferred mice. Transcript levels of TNFα (R), p40 (S), iNOS (T), and IL-6 (U). Results are expressed as the mean ± SD. *p < 0.05 via Student’s or Aspin–Welch t-test vs. sham-treated group. n = 4 animals per group. (V) Effect of the interaction with M1 macrophages on the production of interferon (IFN)-γ protein in the co-culture supernatant. After 48 h, protein levels were determined using ELISA. Results are expressed as the mean ± SD. **p < 0.01 via Dunnett’s test vs. T cell only culture group. n = 3 animals per group