Fig. 3

High expression of XLOC_004787 upregulated the proteins levels related with migration, proliferation and EMT in GC cells. A, C and G By western blotting analysis, the level of E-cadherin increased and the standard of mmp9, Snail, Vimentin, β-catenin, C-myc, Cyclin D1 and TGF-β decreased after XLOC_004787 knockdown in SGC-7901 cells. B, D and H By western blotting detection, the standard of E-cadherin declined and the level of mmp9, Snail, Vimentin, β-catenin, C-myc, Cyclin D1 and TGF-β raised after XLOC_004787 overexpression in HGC-27 cells. E and I Western blotting found that knock down XLOC_004787 in SGC-7901 cells reduced the expression of P-Gsk3β and P-Smad2/3 relative to Gsk3β and Smad2/3. F and J Western blotting showed that upregulated XLOC_004787 in HGC-27 cells enhanced the expression of P-Gsk3β and P-Smad2/3 relative to Gsk3β and Smad2/3. Data were shown as mean ± SEM. The experiments were repeated at least three times. *P < 0.05, **P < 0.01, ***P < 0.001