Fig. 2

SMC1A promoted gastric cancer cell proliferation, invasion and migration. A The expression of SMC1A was examined in SMC1A silenced and overexpressed cells by western blot method. CCK-8 assay (B) and Colony formation assay (C) were used to determine the effect of SMC1A knockdown and overexpression on cell proliferation. Matrigel invasion assay (D) and Wound healing assay (E) analyzed the effect of SMC1A knockdown and overexpression on cell invasion and migration respectively. F Subcutaneous xenografts of AGS cells transfected with SMC1A overexpression plasmids. Images of tumors from nude mice at autopsy are presented (left), the tumor volumes were measured at the indicated time points (middle), and the average weight of the xenografted tumors was measured (right). *P < 0.05, **P < 0.01, ***P < 0.001