Fig. 5

Elevated plasma and intestinal M-CSF levels in UC patients with symptoms of anxiety/depression. A The plasma M-CSF, GM-CSF and MCP-1 levels detected by Luminex in HC (n = 10), UC (n = 16) and UD (n = 20) patients; B the expression of intestinal M-CSF protein in HC (n = 4), UC (n = 4), UD (n = 4) patients. The GAPDH and M-CSF protein expression was detected by Western-Blots. Full-length blots/gels are presented in Additional file 1: Fig. S1; C the relative expression levels of M-CSF RNA in HC (n = 4), UC (n = 5) and UD (n = 6) patients. HC: healthy controls; UC: UC patients without symptoms of anxiety/depression; UD: UC patients with symptoms of anxiety/depression. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001