Fig. 2

Immunophenotypes and phagocytosis of monocytes analyzed by flow cytometry. A FSC-A/FSC-H showed the circle effective single cells, FSC-A/SSC-A showed the population of peripheral blood mononuclear cells (PBMC), CD45/SSC showed the expression of CD45, cells in P1 were monocytes; B the proportions of classic, intermediate and nonclassical monocytes in the HC, UC and UD groups were compared, respectively. CD14 and CD16 are used as markers to distinguish between classical, intermediate and non-classical monocyte immunophenotypes. C Beads + cells represented monocytes that engulfed beads in HC, UC, and UD, respectively. Monocyte phagocytosis was compared among the three groups; HC: healthy controls, n = 13; UC: UC patients without symptoms of anxiety/depression, n = 19; UD: UC patients with symptoms of anxiety/depression, n = 23, **P < 0.01, ***P < 0.001, ****P < 0.0001