Fig. 4

The effect of NRF-1 on E2F1 mRNA. Venn diagram of overlapped genes by GO (a) and Reactome analysis (d). PPI network constructed from the overlap datasets of GO (b) and Reactome (e). c, f The significant module identified from the PPI network using the MCODE method. HepG2 cells were transfected with siNRF1 (g) or pcDNA3-NRF1 (h). The mRNA levels of NRF1, E2F1, CCND1, CCND3, CCNA1, CCNE1 and CDK2 were detected by real-time PCR using β-actin as a control (n = 3). The data represent means ± SD. *P < 0.05, **P < 0.01 and ****P < 0.0001 compared with the control