Fig. 1
From: Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1 polarization
Free fatty acid-mediated macrophage M1 polarization in vitro. Mouse abdominal macrophage were isolated by high-sugar DMEM and then inoculated into a sterile Petri dish (2.5 × 105/ml). Palmitic acid (PA, 500 μM) was used for fatty acid stimulation. A Inflammation factors were detected by ELISA in macrophage culture medium, which was stimulated by PA, and B quantitative cellular expression was measured. Data are shown as the mean ± SEM, n = 3. *P < 0.05, ANOVA. Protein levels of ASC, IL-1β, IL-18 and NLRP3 were performed by western blot in macrophage stimulated by PA (C). Flow cytometry to assess polarization in macrophage stimulated by PBS (D, E) or PA (F, G). Anti-F4/80 (D, F), anti-CD11c, and anti-CD206 (E, G). CD11c indicates M1 macrophage, CD206 indicates M2 macrophage. Marker genes of macrophage polarization were detected by qRT-PCR (H) and western blotting (I). Data are shown as the mean ± SEM, n = 3. *P < 0.05, ANOVA