Fig. 2
From: Intestinal Claudin-7 deficiency impacts the intestinal microbiota in mice with colitis
Effect of Cldn7 gene knockout on mucus layer and translocation of intestinal bacteria. A AB-PAS staining shows different kind of goblet cells containing: mixed (black arrow), neutral (purple arrow) and acid (red arrow) mucins. B, C Representative immunohistochemical images of Muc2 in control tissues and DSS-treated colonic tissues from CreW and CreERT2 mice, the red arrows represent positive cells. Right: the mean optical density (MOD) of Muc2 positive cells (**P < 0.01, ***P < 0.001, n = 3). D, E Bacteria were stained with EUB338 by fluorescent in situ hybridisation (FISH) in each group, the red arrows represent bacteria. Right: quantification the number of bacteria per mm2 in each group (*P < 0.05, n = 3)