Fig. 7

Linkage between results from the public dataset and results from our transferability study. a Venn diagram shows the 1781 genes that were identified to include significant AS events in the transferability study that were overlapped with genes of results from GSE66207. b IGV Sashimi plot showing IRF1 and STAT3 also had significant tandem 3’UTR events, as well as significantly altered expression, in patients with CD compared to the controls. c Quantitative real-time PCR (RT-qPCR) analysis showing that the exon1 mRNA expression of the STAT3 and IRF1 were both significantly upregulated in the CD group. ***Student’s t-test P value < 0.001; bar = SEM. d Scheme shows how primers were designed to target the lengthened 3’UTR of the STAT3 and IRF1 genes in the CD patients. e RT- qPCR analysis results show that the expression of the lengthened 3’UTR region of STAT3 and IRF1 were significantly upregulated in the CD samples, matching the results of b. ***Student’s t-test P value < 0.001; bar = SEM. f Normalized results of the lengthened 3’UTR/exon 1 expression of the STAT3 and IRF1 gene. ***Student’s t-test P value < 0.001; bar = SEM