Fig. 4

Longitudinal T cell profiling of subjects by flow cytometry and intracellular cytokine staining. a Representative gating scheme showing MAIT cell identification and their cytokine production; b Frequency of total lymphocytes within peripheral blood mononuclear cell isolations and their CD4:CD8 T cell ratios. c, d Comparison of T regulatory and MAIT cell frequencies between UC patients and healthy controls (HC) with longitudinal frequencies and INFγ+, IL-17A+, and IL-10+ proportions displayed by treatment group and clinical response (black, placebo; red, non-responser; green, responser). Each line is an individual subject. Controls are displayed with placebo results (C). Between-group comparisons were conducted using two-sample t tests and p values < 0.05 considered significant