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Table 2 qPCR analysis of the expression levels of 27 markers in tumour tissue compared to non-tumour, using β-actin as endogenous control

From: mRNA profiling of a well-differentiated G1 pancreatic NET correlates with immunohistochemistry profile: a case report

Marker

Expression level

CgA

+ + +*

synaptophysin

=

NSE

+

CD56

+ +*

Ki-67

− − −*

PCNA

=

DAXX

=

CD24

−*

CD44

−*

CD31

− −*

CD45

+*

Vimentin

+

β-catenin

+ +*

MENA

− −*

CD49

−*

Laminin

=

ECAD

− −*

PCAD

− − −*

EpCAM

− −*

CDX2

− −*

PDX1

+ +*

CD14

=

CK6

− −*

CK7

− − −*

CK13

−*

CK20

+ +*

P53

+ +*

  1. + and − indicates whether marker mRNA transcript levels are higher or lower in the tumour tissue compared to the matched no-tumour tissue. *Represents significant changes (p < 0.05)
  2. −: 1–twofold change lower compared to normal; − −: 2–tenfold change lower compared to normal; − − −: > tenfold change lower compared to normal; +: 1–twofold change higher relative to normal; + +: 2–tenfold change higher relative to normal; + + +: > tenfold change relative to normal; =: similar in tumour and non-tumour