Fig. 5
From: CDX2 and Reg IV expression and correlation in gastric cancer
CDX2 promoted the migration and invasion of gastric cancer cells. a–d Wound-healing assay to detect the migration of AGS and MKN-45 cells after CDX2 silencing or overexpression. Representative microscopic images are shown. Magnification 100 × . The bar charts show the percentage of wound-healing at indicated times (below panel). e–h Effects of CDX2 silencing or overexpression on the migration and invasion of both cell lines were assessed by Transwell assay. Cells transfected with CDX2 siRNA or pcDNA4/TO-CDX2 migrated across uncoated or Matrigel-coated Transwell inserts. Cells in five fields from each membrane were counted using an optical microscope. Representative images of migrated cells are shown. Magnification 200×. The bar charts show the number of cells that migrated across 8 µm pores to the underside of the membrane. Data are presented as mean ± SD. *p < 0.05; **p < 0.01 compared with the control groups