Fig. 5

Alpha (a, d) and beta (b, c, e, f) diversity metrics of cecal (a–c) and fecal (d–f) microbiota of mice undergoing cholecystectomy (XGB) followed by either a sham (SHM) or ovariectomy (OVX) operation. Means in a, c are represented by a diamond. In a–c, wk 12, n = 4–5 per group; wk 18, n = 5 per group; wk 24, n = 7–8 per group. In d–f, n = 7–13 per group. b, e unweighted Unifrac distance principal coordinate analysis. c, f weighted Unifrac distance principal coordinate analysis. In b, c, e, f, each dot represents a single sample. Larger dots are specific to wk 24. Main effects of surgery are denoted by *(p < 0.05). Trends are denoted by +(p < 0.10)