Fig. 4

Representative in situ hybridization (ISH) of miR-4454, miR-106-5p, miR-17-5p in tissue sections of pancreatic cancers. ISH assays demonstrate a significant miRNA expression dysregulation among different tumor hystotypes. Normal grey matter specimens showed a negative/faint expression of miR-106-5p and miR-17-5p in CP. On the other hand, IPMN and AVC showed a moderate/strong expression of miR-106-5p and miR-4454. Columns denote the different tumor subtype while rows the different miRNAs analyzed. The presence of miRNA is shown by a grainy blue cytoplasmatic stain; slides counterstained in fast red. (Scale bars 200 μm; Original magnifications 10x and 5x)