Fig. 3

Metaplasia in gastric mucosa of FVB-CA-AhR and wild-type mice. (a and b) Proper gastric glands in 16 wk. males revealed by HE staining. (c) PAS⁺ area in the proper gastric glands in 16 wk. males revealed by PAS staining. (d) Relative PAS⁺ area in the proper gastric glands. (e) AB pH 2.5⁺ area in proper gastric glands in 16 wk. males revealed by AB pH 2.5 staining. (f) Relative AB pH 2.5⁺ area in the proper gastric glands. (g) Correlation between IM cysts and intestinal metaplasia. Graph shows the Spearman’s rank correlations between the number of IM cysts and relative AB pH -2.5⁺ area in the proper gastric glands. *P < 0.05, Spearman’s rank correlation test; ρ Spearman’s rank correlation coefficient; CA-AhR: FVB-CA-AhR. In a, d, and e, the circles denote metaplastic areas in serial sections. In graphs, blue and red asterisks denote significant strain difference with wild-type (WT) mice in male and female at the same age, respectively (Mann-Whitney-U test, *P < 0.05, **P < 0.01). Sharp denote significant sex-related difference in the same strains at the same age (Mann-Whitney-U test, #P < 0.05). Bars denote 200 μm (a, c, and e) and 50 μm (b)