Fig. 4

Expression of the co-receptors LRP5/6 and protein levels of β-catenin, GSK3β and Akt. Expression of LRP5 and LRP6 was analyzed in EPC-1 and EPC-2, CP-A, CP-B, OE33 and OE19 cells by quantitative Real time RT-PCR. Along Barrett’s sequence, an increase of LRP5 (a) and LRP6 (b) levels was detected with highest expression levels in OE19. Protein level expression of pAkt/Akt, pβ-catenin/β-catenin and pGSK3β/GSK3β was analyzed in EPC-1 and EPC-2, CP-A, CP-B, OE33 and OE19 cells by westernblot analysis. A significant higher pβ-catenin expression was found in EPC-2. (c). Elevated levels of pGSK3β were detected in EPC-1, EPC-2, and OE19 (d). EPC-1, EPC-2 and CP-A expressed only marginal levels of pAkt. A loss of pAkt expression was detected in OE33 (e). Normalization was done with β-Actin. Values are shown as mean ± S.E.M. (One-way-ANOVA with Bonferroni correction, * - p < 0.05, **- p < 0.01 compared to EPC-1)