Fig. 2From: Transplantation, gene therapy and intestinal pathology in MNGIE patients and miceSmall intestinal histopathology in MNGIE mice. a, b Hematoxylin-Eosin (H&E) stains of the small intestine of young (2-month-old) control mice (a) and age-matched Tympā/-Upp1ā/ā mutants (b) show normally layered organization of the intestinal wall in both groups. c, d H&E stains of the small intestine show normal thickness of the tunica muscularis propria in old (12-month-old) control mice (c), whereas in age-matched Tympā/-Upp1ā/ā mutants (d) the muscle wall is atrophic. e Bone marrow cell chimerism and vector copy number in recipients of 0.5āĆā106 Lin- cells transduced by LV-PGK-TP (MOI10) (nā=Ā 3 mice). f Quantification of Thd and d-Urd in urine of untreated controls and age-matched recipients 6 and 11Ā months after transplantation (nā=Ā 3 mice). g H&E stain of the small intestine shows that atrophy of the tunica muscularis propria is prevented in old (12-month-old) Tympā/-Upp1ā/ā mice 10Ā months after treatment. h Quantification confirms atrophy of the muscle wall in 12-month-old Tympā/-Upp1ā/ā mice compared to wild-type age-matched controls. Treatment is associated with normal thickness of the tunica muscularis propria. i Quantification of the number of myenteric ganglion cell groups per tissue section shows progressive loss of ganglion cells in Tympā/-Upp1ā/ā mice, without effect of the treatment. Nā=Ā 2ā4 mice/group; in all graphs lines represent the median; *Pā<ā0.05, **Pā<ā0.01, ***Pā<ā0.001. Original magnification (a-d and g): 200xBack to article page