Fig. 3

Apatinib inhibit migration and invasion of QBC939 and TFK-1 cells. a QBC939 and TFK-1 were treated with apatinib (0, 10, 100, 1000, 10000 nM, respectively) for 48 h. the relative cell viability was detected by MTT assay. Data shown are means ± SD (n = 3). *P < 0.05, **P < 0.01 in QBC939 and TFK-1 cells versus control group (0 nM apatinib). b Wound healing on QBC939 cells and TFK-1 cells treatment with or without 100 nM apatinibfor 24 h. The migration index (the ratio of migration distance to total distance) was used to measure the movement ability. c The cells were treated with apatinib (100 nM) for 24 h. The invasion cells were stained. d The cells were treated with apatinib (100 nM) for 24 h. The protein expression of Slug, snail and MMP9 in QBC939 cells and TFK-1 cells were measured by western blot. GAPDH was included as a loading control. *P < 0.05, **P < 0.01 vs control group (0 nM apatinib)