Skip to main content
Fig. 3 | BMC Gastroenterology

Fig. 3

From: Expression profiling and intracellular localization studies of the novel Proline-, Histidine-, and Glycine-rich protein 1 suggest an essential role in gastro-intestinal epithelium and a potential clinical application in colorectal cancer diagnostics

Fig. 3

PHGR1 protein characterization. a Western blot of PHGR1 protein in the colorectal cancer cell lines LS174T (lane 1), HT29 (lane 2), and HCT116 (lane 3) and normal mucosa samples (lanes 4–6). GAPDH was included as a loading control. b Western blot of untreated (U) and chemically deglycosylated (DG) protein extracts from the LS174T cell line, stained with a polyclonal antibody against PHGR1. The volumes loaded corresponded to the same volume of original protein extract. c Immunohistochemical staining of PHGR1 in colon and colorectal cancer. 1) PHGR1-stained (brown) normal colon (2.5× microscope objective). 2) PHGR1-stained normal colon mucosa (20× objective). 3) PHGR1-stained normal mucosa (100× objective), upper part of a crypt. The arrows indicate goblet cells. 4) PHGR1-stained colorectal carcinoma (20× objective). Nuclear counterstaining was achieved with hematoxylin (blue). d Relative PHGR1 and alkaline phosphatase (AP) mRNA levels during Caco-2 cell differentiation. mRNA levels were normalized both against the reference mRNA HPRT1 and the day 0 sample. Data are represented as mean values from three biological replicates. The error bars represent standard deviation. e Western blot of Caco-2 cells cultured to confluence and beyond (0–14 days) using antibodies against PHGR1 (upper panel) and GAPDH (lower panel)

Back to article page

BMC Gastroenterology

ISSN: 1471-230X

Contact us