Table 1 Primer sequences (5′ to 3′) used for the quantitative polymerase chain reaction
Gene | Forward primer | Reverse primer |
|---|---|---|
ZO-1 | CCATCTTTGGACCGATTGCTG | TAATGCCCGAGCTCCGATG |
Occludin | ACGGTGCCATAGAATGAGATGTTG | CAGCTAGTTGTTCATTTCTGCACCA |
Claudin-1 | CATCGCAGCTACTTGCCAGT | TTTTTTTTTTTTTTTTGCAAAAACGA |
IL-1 | TCAGGAAGGCAGTGTCACTCATTG | ACACACTAGCAGGTCGTCATCATC |
IL-6 | TAGAGTCACAGAAGGAGTGG | GCCAGTTCTTCGTAGAGA |
IL-7 | AACCTCCAAGAAACTACTGCC | CACCGATGCATGGTTGCTAAC |
IL-10 | CTCAGCACTGCTATGCTGCCTGCT | TCTTCACCTGCTCCACTGCCTTGC |
INF-γ | TGTCATCGAATCGCACCT | TCAGCACCGACTCCTTTT |
TNF-α | ACCCCCAACCTATGAAGAAA | TCCACGCAAAACGGAATGAA |
GADPH | CCGAGGGCCCACTAAAGG | GCTGTTGAAGTCACAGGAGACAA |