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Table 2 Characteristics of mice in the 12-week model

From: Deficiency of eNOS exacerbates early-stage NAFLD pathogenesis by changing the fat distribution

 

12-week model

eNOS+/+BD

eNOS-/-BD

eNOS+/+HFD

eNOS-/-HFD

Number of animals

8

8

8

8

Body weight (g)

28.1 ± 1.9

27.2 ± 2.4

42.8 ± 3.1*

41.7 ± 2.4

Liver weight (g)

1.2 ± 0.1

1.2 ± 0.2

2.3 ± 0.6*

2.8 ± 0.5§

Visceral fat weight (g)

0.6 ± 0.1

0.7 ± 0.3

2.0 ± 0.3*

1.2 ± 0.3§

Liver weight/body weight ratio (%)

4.2 ± 0.3

4.3 ± 0.6

5.2 ± 1.0*

6.7 ± 1.0§

Visceral fat weight/body weight ratio (%)

2.1 ± 0.4

2.4 ± 1.0

4.7 ± 1.0*

3.0 ± 0.6§

Serum ALT (IU/L)

9.2 ± 0.8

17.5 ± 9.3

74.8 ± 41.4*

172.8 ± 58.2§

Fasting glucose (mg/dL)

89.6 ± 8.0

85.7 ± 20.6

151.8 ± 36.4*

135.8 ± 32.3

Fasting insulin (ng/mL)

0.72 ± 0.41

0.57 ± 0.72

1.63 ± 0.81*

2.10 ± 0.74

HOMA-IR

2.9 ± 1.8

2.0 ± 2.5

11.0 ± 6.7*

13.3 ± 6.6

Serum cholesterol (mg/dL)

90.5 ± 3.8

85.1 ± 4.9

174.8 ± 22.9*

263.0 ± 28.5§

Serum TG (mg/dL)

24.8 ± 6.8

26.0 ± 5.6

31.3 ± 5.9*

33.8 ± 8.2

Serum NEFA (mEq/L)

1.2 ± 0.0

1.1 ± 0.2

1.8 ± 0.1*

1.7 ± 0.3

Serum leptin (ng/mL)

3.9 ± 2.0

3.5 ± 2.7

30.2 ± 4.4*

31.6 ± 22.9

  1. BD basal diet, HFD high-fat diet, HOMA-IR homeostasis model assessment of insulin resistance, TG triglyceride, NEFA nonesterified fatty acid
  2. Data are expressed as the mean ± SEM
  3. Significant differences exist between *eNOS+/+ and BD vs. eNOS+/+ and HFD; §eNOS+/+ and HFD vs. eNOS-/- and HFD for listed parameters at the P < .05 level