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Table 2 Characteristics of mice in the 12-week model

From: Deficiency of eNOS exacerbates early-stage NAFLD pathogenesis by changing the fat distribution

  12-week model
eNOS+/+BD eNOS-/-BD eNOS+/+HFD eNOS-/-HFD
Number of animals 8 8 8 8
Body weight (g) 28.1 ± 1.9 27.2 ± 2.4 42.8 ± 3.1* 41.7 ± 2.4
Liver weight (g) 1.2 ± 0.1 1.2 ± 0.2 2.3 ± 0.6* 2.8 ± 0.5§
Visceral fat weight (g) 0.6 ± 0.1 0.7 ± 0.3 2.0 ± 0.3* 1.2 ± 0.3§
Liver weight/body weight ratio (%) 4.2 ± 0.3 4.3 ± 0.6 5.2 ± 1.0* 6.7 ± 1.0§
Visceral fat weight/body weight ratio (%) 2.1 ± 0.4 2.4 ± 1.0 4.7 ± 1.0* 3.0 ± 0.6§
Serum ALT (IU/L) 9.2 ± 0.8 17.5 ± 9.3 74.8 ± 41.4* 172.8 ± 58.2§
Fasting glucose (mg/dL) 89.6 ± 8.0 85.7 ± 20.6 151.8 ± 36.4* 135.8 ± 32.3
Fasting insulin (ng/mL) 0.72 ± 0.41 0.57 ± 0.72 1.63 ± 0.81* 2.10 ± 0.74
HOMA-IR 2.9 ± 1.8 2.0 ± 2.5 11.0 ± 6.7* 13.3 ± 6.6
Serum cholesterol (mg/dL) 90.5 ± 3.8 85.1 ± 4.9 174.8 ± 22.9* 263.0 ± 28.5§
Serum TG (mg/dL) 24.8 ± 6.8 26.0 ± 5.6 31.3 ± 5.9* 33.8 ± 8.2
Serum NEFA (mEq/L) 1.2 ± 0.0 1.1 ± 0.2 1.8 ± 0.1* 1.7 ± 0.3
Serum leptin (ng/mL) 3.9 ± 2.0 3.5 ± 2.7 30.2 ± 4.4* 31.6 ± 22.9
  1. BD basal diet, HFD high-fat diet, HOMA-IR homeostasis model assessment of insulin resistance, TG triglyceride, NEFA nonesterified fatty acid
  2. Data are expressed as the mean ± SEM
  3. Significant differences exist between *eNOS+/+ and BD vs. eNOS+/+ and HFD; §eNOS+/+ and HFD vs. eNOS-/- and HFD for listed parameters at the P < .05 level