Fig. 2
From: Deficiency of eNOS exacerbates early-stage NAFLD pathogenesis by changing the fat distribution
Analysis of liver steatosis and inflammation. a Oil-red O staining (red color) shows lipid deposits in liver samples. At 12 weeks, macrovesicular steatosis was visible in the eNOS-/-/HFD mice and microvesicular steatosis was visible in the eNOS+/+/HFD mice. Scale bar, 200 μm. b The liver TG content was significantly higher in the eNOS-/-/HFD mice than in the eNOS+/+/HFD mice. The liver TG content was significantly higher in the eNOS+/+/HFD mice than in the eNOS+/+/BD mice, which were regarded as the control group. c Liver samples stained using H&E. An evaluation of the number of inflammatory foci per field and the amount of ballooning revealed that the eNOS-/-/HFD mice exhibited severer liver inflammation than the eNOS+/+/HFD mice at 12 weeks. Scale bar, 200 μm. d and e Liver samples stained using myeloperoxidase (d) and Sirius red (e) showed no significant positive findings. (Data were expressed as the mean ± SEM. *P < 0.05, represents a significant difference; n.s. represents no significant difference.)