Table 1 Primers used to determine the expression of the mRNAs for human (h) and/or mice (m) of interest

FZD1(h),

5’-TTGTGGGCATCACATCGGGCTT-3’,

5’-TGCAAAAGTCAGTGGCTGGCGA-3’;

FZD1(m),

5’-TTGTGGGCATCACATCGGGCTT-3’,

5’-TGCAAAAGTCAGTGGCTGGCGA-3’;

FZD2(h),

5’-AGTTCGGTTTTCAGTGGCCCGA-3’,

5’-TCACGCTCGCCCAGAAACTTGT-3’;

FZD2(m),

5’-ACGCGCAGAGAATGGGCAACAA-3’,

5’-AACGGAAGCGCCAAAGATCCCA-3’;

FZD3(h),

5’-TGTTCTCGGGATTTCCGGCCTT-3’,

5’-GGCTCATCACAATCTGGGAACCTAC-3’;

FZD3(m),

5’-TGCAGCTTTAGCAATGGAGCCCT-3’,

5’-ATCTTCAGGCCACGGGACACCAAA-3’;

FZD4(h),

5’-TGCAGGGTGGGATTTGAGCTGTGA-3’,

5’-AAACAGCAGACAGCGCACCACA-3’;

FZD4(m),

5’-TGCAAACTGGGGGTGTCTGCTA-3’,

5’-TGGTCACGTTGTAGCCGAGGTT-3’;

FZD5(h),

5’-AACAGCATCCGTGACCTGCACT-3’,

5’-TGCAGAGGGAGCAGGCAATTCT-3’;

FZD5(m),

5’-ATCCTCCGAGAGTTCTGTCCTTGG-3’,

5’-TCGTCTCCTTCTTCCCTTTGCCT-3’;

FZD7(h),

5’-CGTGCCAACGGCCTGATGTACTTT-3’,

5’-TGAAGTAGCAGCCCGACAGGAAGA-3’;

FZD7(m),

5’-TGCAGTCATGGCGTCGCTTCTA-3’,

5’-TTCGGCCACTGCCTTTGCCTTT-3’;

FZD9(h),

5’-ACCTGGTGCTGGGCAGTAGTTT-3’,

5’-GCCAGAAGTCCATGTTGAGGCGTT-3’;

FZD9(m),

5’-TGTGTGGTCCGCGTTGTGTT-3’,

5’-TGCAGCCTGTGTTTTCCAGACC-3’;

WNT1(h),

5’-TCGCGGGCAACAACCAAAGT-3’,

5’-ACGTTCACAATACCCCACCATCGG-3’;

WNT1(m),

5’-AACCGCAGCACAGAACCAGCAA-3’,

5’-AACCGCAGCACAGAACCAGCAA-3’;

WNT2(h),

5’-AAGATGGGAAGCGCCAAGGACA-3’,

5’-TTACAGCCTTCCTGCCAGCTCT-3’;

WNT2(m),

5’-AGGTCAGCTCTTCATGGTGGTACA-3’,

5’-AGGGTGTTGCAGTTCCAGCGAT-3’;

WNT3A(h),

5’-TGTTGGGCCACAGTATTCCTCCCT-3’,

5’-AAAGGCCGACTCCCTGGTAGCTTT-3’;

WNT3A(m),

5’-AGCCTGCTGTTGAGGCAATGGT-3’,

5’-AGCGCGAACGCAAAGTTCCA-3’;

WNT5A(h),

5’-ATTCTGGCTCCACTTGTTGCTCGG-3’,

5’-CCTAGCGACCACCAAGAATTGGCTT-3’;

WNT5A(m),

5’-ACGCCTGTGCAACAAGACCTCA-3’,

5’-AAGGGCAGGCACACCACTATTTGC-3’;

WNT11(h),

5’-AGGATGTGGCTGCTGACCTCAA-3’,

5’-TGTTGCACTGCCTGTCTTGTGTCC-3’;

WNT11(m),

5’-TGTTGCACTGCCTGTCTTGTGTCC-3’,

5’-TGGAGCGGAATCCTGTGTTCCCAA-3’;

DKK1(h),

5’-AACTCGGTTCTCAATTCCAACGCT-3’,

5’-GGTACGGCTGGTAGTTGTCAATGG-3’;

DKK1(m),

5’-TGCATGAGGCACGCTATGTGCT-3’,

5’-ACGGAGCCTTCTTGTCCTTTGGT-3’;

LRP6(h),

5’-TGGATCAACCCAGAGCTATTGCCTT-3’,

5’-ACCACTGCCTGCCGATTTGTT-3’;

LRP6(m),

5’-TGGATCAACCCAGAGCTATTGCCTT-3’,

5’-ACTGCCTGCCGGTTTGTTCCAT-3’;

APC(h),

5’-ACTCGGAAATGGGGTCCAAGGGTA-3’,

5’-TGACCGCAGTTTTACTCCAGGGAA-3’;

APC(m),

5’-TGCACAGCGAAGAATAGCCAGGA-3’,

5’-TGCGGTGTTGCTTTCTGCCACT-3’;

TCF1(h),

5’-ATGTGACCCAGAGCCCCTTCAT-3’,

5’-TCTGAGGTGAAGACCTGCTTGGTG-3’;

TCF1(m),

5’-AGCCGTGGTGGAGTCACTTCTT-3’,

5’-TGGGTGAATTGCTGAGCCACCT-3’;

LEF1(h),

5’-CAGATTCTTGGCAGAAGGTGGCAT-3’,

5’-AGCTGTCATTCTTGGACCTGTACCT-3’;

LEF1(m),

5’-CTGCCTACATCTGAAACATGGTGGT-3’,

5’-TGCTGTCAGTGTTCCTTGGGGT-3’;

GSK3B(h),

5’-ATTTCACCTCAGGAGTGCGGGTCT-3’,

5’-TGCAGGTGTGTCTCGCCCATTT-3’;

GSK3B(m),

5’-ACCAAATGGGCGAGACACACCT-3’,

5’-TTAGTATCTGAGGCTGCTGTGGCGT-3’;

CTNNB1(h),

5’-TCGAAATCTTGCCCTTTGTCCCGC-3’,

5’-ACCCCCTCCACAAATTGCTGCT-3’;

CTNNB1(m),

5’-TCCTTGCTCGGGACGTTCACAA-3’,

5’-TGCGTATGTTGCCACGCCTTCA-3’;

DVL1(h),

5’-ATCTCGCCACCCTGAACCTCAACA-3’,

5’-TGCTTTTGCTCCCTTCACTCTGCTG-3’;

DVL1(m),

5’-TGCTCTTGCTCCCTTCACTCTGCT-3’,

5’-TGTCTTTGGCGACCTGTGCAGT-3’;

NFATC1(h),

5’-TCAGCGGAGGAAGAACACTATGGC-3’,

5’-ACAGGCCCAAGCACGAGGTTAT-3’;

NFATC1(m),

5’-TCGTGGAGAAGCAGAGCACAGACA-3’,

5’-CCACCAGGGAATTTGGCTTGCACA-3’;

IL-10(h),

5’-TCCTTGCTGGAGGACTTTAAGGGTT-3’,

5’-AAGGCATTCTTCACCTGCTCCACG-3’;

IL-10(m),

5’-TGCACTACCAAAGCCACAAGGCA-3’,

5’-CGACTGGGAAGTGGGTGCAGTTAT-3’;

TGF-β(h),

5’-ATGATCGTGCGCTCCTGCAA-3’,

5’-TGACACAGAGATCCGCAGTCCT-3’;

TGF-β(m),

5’-TCGACATGGAGCTGGTGAAACGGA-3’,

5’-TAGATGGCGTTGTTGCGGTCCA-3’;

IFN-γ(h),

5’-ACAAGGCTTTATCTCAGGGGCCA-3’,

5’-GCACTGGCTCAGATTGCAGGCATA-3';

IFN-γ(m),

5’-ACGCTACACACTGCATCTTGGCT-3’,

5’-CACCATCCTTTTGCCAGTTCCTCC-3’;

TNF(h),

5’-AGCCCATGTTGTAGCAAACCCTC-3’,

5’-ATGGTGTGGGTGAGGAGCACAT-3’;

TNF(m),

5’-AGCACAGAAAGCATGATCCGCGA-3’,

5’-TGGTGGTTTGTGAGTGTGAGGGT-3’;

IL-2(m),

5’-TTGTCAACAGCGCACCCACTTC-3’,

5’-TTCAATTCTGTGGCCTGCTTGGGC-3’;

IL-15(m),

5’-TCTGCGCCCAAAAGACTTGCAG-3’,

5’-TCAAGGTGGATTCTCTCTGAGCTGT-3’;

IL-2RB(m),

5’-ATGTCACAACCTGCCACGTCCA-3’,

5’-AGGCGAAGGTTGTCAAAGGGATGG-3’;

IL-15RA(m),

5’-GGCCTGGTACATCAAATCAAGGCAG-3’,

5’-ACATTGGGCTTTCTCCTGTGTCCA-3’;

IL-12(m),

5’-TCCCGAAACCTGCTGAAGACCA-3’,

5’-CCAGGCAACTCTCGTTCTTGTGT-3’;

IL-4(m),

5’-ACGTCCTCACAGCAACGAAGAACA-3’,

5’-TCCAGGCATCGAAAAGCCCGAA-3’;

IL-22(m),

5’-AGCCATACATCGTCAACCGCAC-3’,

5’-TGAGTTTGGTCAGGAAAGGCACC-3’;

C-MYC(m),

5’-AGCAGCGACTCTGAAGAAGAGCA-3’,

5’-AGCACTTGCGGTTGTTGCTGAT-3’;

PKCα(m),

5’-TGGGAAGGTGATGCTTGCTGAC-3’,

5’-AGCTGTGTCAGAAATGGCGGCT-3;

β-actin(h),

5’-TGGCATCCACGAAACTACCTTCAAC-3’,

5’-TCTTGATCTTCATTGTGCTGGGTGC-3’;

β-actin(m),

5’-AGCTGAGAGGGAAATCGTGCGT-3’,

5’-TCCATACCCAAGAAGGAAGGCTGGA-3’