Table 1 RT-PCR primer sequences
From: Changes of cytokine levels in a mouse model of post-infectious irritable bowel syndrome
Cytokines | Primer sequence |
|---|---|
Il1b (94 bp) | Forward 5′—ATGGGCAACCACTTACCTATTT—3′ |
Reverse 5′—GTTCTAGAGAGTGCTGCCTAATG—3′ | |
Il10 (116 bp) | Forward 5′—ACAGCCGGGAAGACAATAAC—3′ |
Reverse 5′—CAGCTGGTCCTTTGTTTGAAAG—3′ | |
Il17 (123 bp) | Forward 5′—CGCAATGAAGACCCTGATAGAT—3′ |
Reverse 5′—CTCTTGCTGGATGAGAACAGAA—3′ | |
Ifng (128 bp) | Forward 5′—AAATCCTGCAGAGCCAGATTAT—3′ |
Reverse 5′—GCTGTTGCTGAAGAAGGTAGTA—3′ | |
Actb (470 bp) | Forward 5′—AGGCTGTGCTGTCCCTGTATG—3′ |
Reverse 5′—GAGGTCTTTACGGATGTCAACG—3′ |