Figure 5
Mucosal colonic tissues from control and ALF mice were analyzed for ZO-1 expression. Western blot analyses indicated that ZO-1 expression was altered in mice with ALF. ZO-1 expression was significantly decreased at 9 h in GalN/LPS-treated mice (20.11 ± 7.37%) and GalN/TNF-α-treated mice (35.47 ± 7.34%) compared with the NS control (100.00 ± 0.00%) and the other groups. No differences were observed in actin expression in the ALF model compared to the control. ZO-1 expression was unchanged at 2 h in the ALF group compared with the NS control. Each bar represents the mean ± SE (n = 3 mice per group). Insets: representative Western blots. Lane 1, NS; lane 2, 2 h after GalN/LPS administration; lane 3, 6 h after GalN/LPS administration; lane 4, 9 h after GalN/LPS administration; lane 5, LPS; lane 6, D-GalN; lane 7, anti-TNF-α-treated group; lane 8, anti-TNF-α R1-treated group; lane 9, TNF-α-treated group; lane 10, 9 h after GalN/TNF-α administration. Statistical significance was determined using a one-way ANOVA followed by the Tukey test. *P < 0.05, **P < 0.01 vs. the NS control group.