GP2 glycosylation with mannose residues is required for bacteria binding. (A) SDS-PAGE of GP2 and uromodulin (UMOD) proteins with (+) and without (-) deglycosylation with peptide:N-glycosidase F treatment. (B) Binding of Type 1 fimbriated bacteria (AAEC185/pSH2) to glycosylated and deglycosylated GP2 or UMOD. (C) AAEC185/pSH2 bacteria binding to GP2 after pre-incubation with 1% (v/v) α-D-mannose. Data are expressed as the mean CFU/well of triplicate samples and the error bar represents 1SD. ** p < 0.01 compared with untreated controls.