Figure 2
From: The pancreatic zymogen granule membrane protein, GP2, binds Escherichia colitype 1 Fimbriae
Specificity of GP2 binding to bacteria with Type 1 fimbria. (A) AAEC185/pSH2 bacteria binding assay with microtiter wells previously coated with 100 ng of GP2 protein followed by preincubation with either rabbit anti-human GP2 antiserum or control preimmune serum. (B) 5 μg of GP2 protein was preincubated with Type 1 fimbriated bacteria (AAEC185/pSH2) before application to the microtiter plates. Data are expressed as the mean CFU/well and the error bars equal 1SD. All assays were performed in triplicate. ** p < 0.01 compared with control preimmune serum (A) or without GP2 preincubation (B).