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Figure 2 | BMC Gastroenterology

Figure 2

From: TNF-α-induced up-regulation of pro-inflammatory cytokines is reduced by phosphatidylcholine in intestinal epithelial cells

Figure 2

This figure represents the effect of both LPC and PC on the association of TNF-α-R1 and TNF-α-R2 to DRMs. COS cells were lysed in 1% Triton X-100 at 4°C 20 h after transient transfection of TNF-α-R1 and TNF-α-R2. After floatation in an OptiPrep step-gradient, TNF-α-R1 and TNF-α-R2 were found in two pools, in DRMs (lane 7–8) and in soluble membranes (lane 1–4). Pre-treatment with 200 μmol LPC or PC resulted in an increased DRM association.

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BMC Gastroenterology

ISSN: 1471-230X

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