Pancreatic Reg/PAP gene expression in wild type and CF mice during acute pancreatitis. Pancreatic tissue was obtained at sacrifice and processed to extract total RNA as described in the Materials and Methods. Gene expression levels were determined by real time quantitative RT-PCR. (A) Reg3α; (B) PAP/Reg3β; (C) Reg3γ. Data are expressed as copies of gene specific mRNA per μg total RNA. Control mice were untreated or injected with 0.9% NaCl. Mice for the 1 h time point received a single injection of caerulein and were sacrificed one hour after injection. The other data are from mice that received 7 hourly caerulein injections and the times are relative to the first injection. Values are presented on semi-log plots as means and sem. (*) p < 0.05 compared to control of the same genotype. (+) p < 0.05 comparing CF to wild type at the same time point. n = 3 to 7 mice per group and time point.