CK isoenzymes in HCC. Subcellular fractions of HCC liver samples no. 4 (A, B) and 9 (C, D) were analysed by cellulose polyacetate electrophoresis as described under "Methods". H = total homogenate; C = cytosolic fraction; M = mitochondrial fraction; Ct = control containing BB-CK and Mi-CK; Mi-CKd = dimeric Mi-CK; Mi-CKo = octameric Mi-CK; O = place of sample application. The cellulose polyacetate strips were exposed to an overlay gel containing PCr (A, C = CK activity staining), or lacking PCr (B, D = corresponding negative controls). Whereas Mi-CKd and Mi-CKo were well separated in (A), they were less so in (C).