Activity and feeding behavior during the first scotophase after LPS injection. Digital video images were scored for activity and mouse induced movement of food pellets during the first scotophase after injection of saline (open bars), 0.1 mg/kg LPS (shaded bars) or 2 mg/kg LPS (black bars) and compared to the total amount of food consumed during the same 12-hour period. Movement data are presented as the percent of the total frames that movement was scored (left axis). Food intake in grams is presented on the right axis. Values are mean ± SEM (n = 5 for saline and 2 mg/kg LPS groups, n = 6 for 0.1 mg/kg group). * P < 0.03 vs. corresponding saline control. ‡ P < 0.05 vs. corresponding 0.1 mg/kg LPS dose.