Figure 6From: Mucosal gene therapy using a pseudotyped lentivirus vector encoding murine interleukin-10 (mIL-10) suppresses the development and relapse of experimental murine colitis Analysis of LV biodistribution by real-time quantitative PCR (RT-qPCR). Genomic DNA was isolated from mouse colonic tissue and vector copy number was quantified using TaqMan qPCR assay to detect the HIV-1 packaging sequences. Genomic DNA from the PC3 cell line, a human prostate cancer cell line, transfected with LV-GFP was used as a positive control.Back to article page